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Article Open Access

A comprehensive analysis of the predicted targets of miR-642b-3p associated with the long non-coding RNA HOXA11-AS in NSCLC cells

  • Authors:
    • Yu Zhang
    • Jie Luo
    • Xiao Wang
    • Han‑Lin Wang
    • Xiu‑Ling Zhang
    • Ting‑Qing Gan
    • Gang Chen
    • Dian‑Zhong Luo
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Medical Oncology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250012, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6147-6160
    |
    Published online on: February 22, 2018
       https://doi.org/10.3892/ol.2018.8105
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Abstract

Long non-coding RNA HOXA11 antisense RNA (HOXA11-AS) has been previously reported to be involved in the tumorigenesis and progression of ovarian cancer and glioma. However, the function of HOXA11‑AS in lung cancer remains unclear. Following the knockdown of HOXA11‑AS in A549 cells, a microarray analysis was performed in order to detect the differences in microRNA (miRNA/miR) profiles. Subsequently, miR‑642b‑3p was selected for further analysis. Four miRNA target prediction algorithms were used to identify potential target genes of miR‑642b‑3p. Bioinformatics analyses, including Gene Ontology (GO), Kyoto Encyclopaedia of Genes and Genomes, protein‑protein interactions (PPIs) and network analysis, were performed to investigate the potential functions, pathways and networks of the target genes. Furthermore, the differential expression of miR‑642b‑3p and its target genes between normal lung and non‑small cell lung cancer (NSCLC) tissues was verified using The Cancer Genome Atlas (TCGA) database. Six target genes [zinc finger protein 350, heterogeneous nuclear ribonucleoprotein U, high mobility group box 1, phosphodiesterase 4D (PDE4D), synaptotagmin binding cytoplasmic RNA interacting protein and basic helix‑loop‑helix family member B9] of miR‑642b‑3p were predicted using all 4 algorithms. It was revealed that miR‑642b‑3p was overexpressed in adenocarcinoma and squamous cell carcinoma tissues compared with non‑cancerous lung tissues based on the TCGA database. From the 6 target genes, PDE4D was downregulated in lung adenocarcinoma and squamous cell carcinoma tissues, and a weak negative correlation between HOXA11‑AS and PDE4D was identified. The area under the curve of PDE4D was 0.905 [95% confidence interval (CI), 0.879‑0.931] for patients with lung adenocarcinoma and 0.665 (95% CI, 0.606‑0.725) for patients with squamous cell carcinoma. Additionally, GO analysis of the target genes revealed that miR‑642b‑3p was specifically involved in complex cellular pathways. The target gene RAN binding protein 2 possessed the highest degree of interactions in the PPI network (degree=40). It was hypothesized that HOXA11‑AS may have a function in NSCLC by regulating the expression of miR‑642b‑3p and PDE4D, which laid the foundation for the further elucidation of the potential molecular mechanisms of NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Luo J, Wang X, Wang HL, Zhang XL, Gan TQ, Chen G and Luo DZ: A comprehensive analysis of the predicted targets of miR-642b-3p associated with the long non-coding RNA HOXA11-AS in NSCLC cells. Oncol Lett 15: 6147-6160, 2018.
APA
Zhang, Y., Luo, J., Wang, X., Wang, H., Zhang, X., Gan, T. ... Luo, D. (2018). A comprehensive analysis of the predicted targets of miR-642b-3p associated with the long non-coding RNA HOXA11-AS in NSCLC cells. Oncology Letters, 15, 6147-6160. https://doi.org/10.3892/ol.2018.8105
MLA
Zhang, Y., Luo, J., Wang, X., Wang, H., Zhang, X., Gan, T., Chen, G., Luo, D."A comprehensive analysis of the predicted targets of miR-642b-3p associated with the long non-coding RNA HOXA11-AS in NSCLC cells". Oncology Letters 15.5 (2018): 6147-6160.
Chicago
Zhang, Y., Luo, J., Wang, X., Wang, H., Zhang, X., Gan, T., Chen, G., Luo, D."A comprehensive analysis of the predicted targets of miR-642b-3p associated with the long non-coding RNA HOXA11-AS in NSCLC cells". Oncology Letters 15, no. 5 (2018): 6147-6160. https://doi.org/10.3892/ol.2018.8105
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Luo J, Wang X, Wang HL, Zhang XL, Gan TQ, Chen G and Luo DZ: A comprehensive analysis of the predicted targets of miR-642b-3p associated with the long non-coding RNA HOXA11-AS in NSCLC cells. Oncol Lett 15: 6147-6160, 2018.
APA
Zhang, Y., Luo, J., Wang, X., Wang, H., Zhang, X., Gan, T. ... Luo, D. (2018). A comprehensive analysis of the predicted targets of miR-642b-3p associated with the long non-coding RNA HOXA11-AS in NSCLC cells. Oncology Letters, 15, 6147-6160. https://doi.org/10.3892/ol.2018.8105
MLA
Zhang, Y., Luo, J., Wang, X., Wang, H., Zhang, X., Gan, T., Chen, G., Luo, D."A comprehensive analysis of the predicted targets of miR-642b-3p associated with the long non-coding RNA HOXA11-AS in NSCLC cells". Oncology Letters 15.5 (2018): 6147-6160.
Chicago
Zhang, Y., Luo, J., Wang, X., Wang, H., Zhang, X., Gan, T., Chen, G., Luo, D."A comprehensive analysis of the predicted targets of miR-642b-3p associated with the long non-coding RNA HOXA11-AS in NSCLC cells". Oncology Letters 15, no. 5 (2018): 6147-6160. https://doi.org/10.3892/ol.2018.8105
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