Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
May-2018 Volume 15 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2018 Volume 15 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Sodium citrate inhibits the proliferation of human gastric adenocarcinoma epithelia cells

  • Authors:
    • Yuan Xia
    • Xulong Zhang
    • Agula Bo
    • Juan Sun
    • Minhui Li
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, College of Life Sciences, Inner Mongolia University, Inner Mongolia Autonomous Region 010021, P.R. China, Department of Immunology, College of Basic Medicine, Capital Medical University, Beijing 100069, P.R. China, School of Public Health, Inner Mongolia Medical University, Inner Mongolia Autonomous Region 010110, P.R. China, Department of National Medicine, Inner Mongolia Institute of Chinese Medicine, Hohhot, Inner Mongolia Autonomous Region 010059, P.R. China
  • Pages: 6622-6628
    |
    Published online on: February 23, 2018
       https://doi.org/10.3892/ol.2018.8111
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The objective of the present study was to investigate the cytotoxic effects of sodium citrate on human gastric adenocarcinoma epithelia AGS cells. Numerous cytotoxicity‑associated sodium citrate‑induced effects were assessed, including cell viability and proliferation, cytokine expression and caspase activity. In vitro studies demonstrated that incubation with sodium citrate (>3.125 mM) inhibited AGS cell viability and proliferation in a dose‑dependent manner. Incubation with sodium citrate for 24 h revealed that the levels of interleukin‑1β (IL‑1β), IL‑8 and tumor necrosis factor increased with an increasing of dose of sodium citrate, whereas the IL‑6 levels exhibited only a slight alteration. In addition, increases in caspase‑3 and ‑9 activities were associated with increased duration of treatment and dosage of sodium citrate. Collectively, the results of the present study demonstrated that treatment with sodium citrate at higher concentrations or for longer durations exerts a cytotoxic effect on AGS cells via the induction of the intrinsic apoptosis pathway and the alteration in the levels of certain cytokines.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Leumann E, Hoppe B, Neuhaus T and Blau N: Efficacy of oral citrate administration in primary hyperoxaluria. Nephrol Dial Transpl. 10 Suppl 8:S14–S16. 1995. View Article : Google Scholar

2 

Phillips R, Hanchanale VS, Myatt A, Somani B, Nabi G and Biyani CS: Citrate salts for preventing and treating calcium containing kidney stones in adults. Cochrane Database Syst Rev: CD010057. 2015. View Article : Google Scholar

3 

Pastorino J, Hansen CL and McMahon DJ: Effect of sodium citrate on structure-function relationships of Cheddar cheese. J Dairy Sci. 86:3113–3121. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Westergaard N, Waagepetersen HS, Belhage B and Schousboe A: Citrate, a ubiquitous key metabolite with regulatory function in the CNS. Neurochem Res. 42:1583–1588. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Wang JY, Zhu SG and Xu CF: Essential Biochemistry. 1st. 20. Higher Education Press; Beijing: pp. 340–342. 2010

6 

Warburg O, Wind F and Negelein E: The metabolism of tumors in the body. J Gen Physiol. 8:519–530. 1927. View Article : Google Scholar : PubMed/NCBI

7 

Warburg O: On the origin of cancer cells. Science. 123:309–314. 1956. View Article : Google Scholar : PubMed/NCBI

8 

Moreno-Sánchez R, Rodríguez-Enríquez S, Marín-Hernández A and Saavedra E: Energy metabolism in tumor cells. FEBS J. 274:1393–1418. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Vander Heiden MG, Cantley LC and Thompson CB: Understanding the Warburg effect: The metabolic requirements of cell proliferation. Science. 324:1029–1033. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Zhang X, Varin E, Allouche S, Lu Y, Poulain L and Icard P: Effect of citrate on malignant pleural mesothelioma cells: A synergistic effect with cisplatin. Anticancer Res. 29:1249–1254. 2009.PubMed/NCBI

11 

Izyumov DS, Avetisyan AV, Pletjushkina OY, Sakharov DV, Wirtz KW, Chernyak BV and Skulachev VP: ‘Wages of fear’: Transient threefold decrease in intracellular ATP level imposes apoptosis. Biochim Biophys Acta. 1658:141–147. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Vander Heiden MG, Chandel NS, Schumacker PT and Thompson CB: Bcl-xL prevents cell death following growth factor withdrawal by facilitating mitochondrial ATP/ADP exchange. Mol Cell. 3:159–167. 1999. View Article : Google Scholar : PubMed/NCBI

13 

Yousefi S, Owens JW and Cesario TC: Citrate shows specific, dose-dependent lympholytic activity in neoplastic cell lines. Leuk Lymphoma. 45:1657–1665. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Wang YS and Wang ZY: Sodium citrate induces apoptosis in biocontrol yeast Cryptococcus laurentii. J Appl Microbiol. 113:135–142. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Sun J, Misumi A, Shimaoka K, Aoki F and Esaki F: Stomach cancer-related mortality. Eur J Cancer Prev. 10:61–67. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Hamashima C: Current issues and future perspectives of gastric cancer screening. World J Gastroenterol. 20:13767–13774. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Wang XQ, Terry PD and Yan H: Review of salt consumption and stomach cancer risk: Epidemiological and biological evidence. World J Gastroenterol. 15:2204–2213. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Reeves GK, Pirie K, Green J, Bull D and Beral V; Million Women Study Collaborators, : Comparison of the effects of genetic and environmental risk factors on in situ and invasive ductal breast cancer. Int J Cancer. 131:930–937. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Sun J, Bi L, Chi Y, Aoki K and Misumi J: Effect of sodium acetate on cell proliferation and induction of proinflammatory cytokines: A preliminary evaluation. Food Chem Toxicol. 43:1773–1780. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Sun J, Aoki K, Wang W, Guo A and Misumi J: Sodium nitrite-induced cytotoxicity in cultured human gastric epithelial cells. Toxicol in Vitro. 20:1133–1138. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Zhang X, Bo A, Chi B, Xia Y, Su X and Sun J: Magnesium sulfate induced toxicity in vitro in AGS gastric adenocarcinoma cells and in vivo in mouse gastric mucosa. Asian Pac J Cancer Prev. 16:71–76. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Lu Y, Zhang X, Zhang H, Lan J, Huang G, Varin E, Lincet H, Poulain L and Icard P: Citrate induces apoptotic cell death: A promising way to treat gastric carcinoma? Anticancer Res. 31:797–805. 2011.PubMed/NCBI

23 

Guo X, Zhang X, Wang T, Xian S and Lu Y: 3-Bromopyruvate and sodium citrate induce apoptosis in human gastric cancer cell line MGC-803 by inhibiting glycolysis and promoting mitochondria-regulated apoptosis pathway. Biochem Biophys Res Commun. 475:37–43. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Barranco SC, Townsend CM Jr, Casartelli JC, Macik BG, Burger NL, Boerwinkle WR and Gourley WK: Establishment and characterization of an in vitro model system for human adenocarcinoma of the stomach. Cancer Res. 43:1703–1709. 1983.PubMed/NCBI

25 

Xintaropoulou C, Ward C, Wise A, Marston H, Turnbull A and Langdon SP: A comparative analysis of inhibitors of the glycolysis pathway in breast and ovarian cancer cell line models. Oncotarget. 6:25677–25695. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Randle PJ, Denton RM and England PJ: Citrate as a metabolic regulator in muscle and adipose tissue. Biochem Soc Symp. 27:87–103. 1968.PubMed/NCBI

27 

Icard P, Poulain L and Lincet H: Understanding the central role of citrate in the metabolism of cancer cells. Biochim Biophys Acta. 1825:111–116. 2012.PubMed/NCBI

28 

Elinos-Báez CM, Maldonado V and Meléndezzajgla J: Caspases: Apoptosis inducing molecules. Gac Med Mex. 139:493–499. 2003.(In Spanish). PubMed/NCBI

29 

Riedl SJ and Shi Y: Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Biol. 5:897–907. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Kruspig B, Nilchian A, Orrenius S, Zhivotovsky B and Gogvadze V: Citrate kills tumor cells through activation of apical caspases. Cell Mol Life Sci. 69:4229–4237. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Kim GY, Park SY, Jo A, Kim M, Leem SH, Jun WJ, Shim SI, Lee SC and Chung JW: Gecko proteins induce the apoptosis of bladder cancer 5637 cell by inhibiting Akt and activating intrinsic caspase cascade. BMB Rep. 48:531–536. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Creagh EM: Caspase crosstalk: Integration of apoptotic and innate immune signalling pathways. Trends Immunol. 35:631–640. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Persson C, Canedo P, Machado JC, El-Omar EM and Forman D: Polymorphisms in inflammatory response genes and their association with gastric cancer: A HuGE systematic review and meta-analyses. Am J Epidemiol. 173:259–270. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xia Y, Zhang X, Bo A, Sun J and Li M: Sodium citrate inhibits the proliferation of human gastric adenocarcinoma epithelia cells. Oncol Lett 15: 6622-6628, 2018.
APA
Xia, Y., Zhang, X., Bo, A., Sun, J., & Li, M. (2018). Sodium citrate inhibits the proliferation of human gastric adenocarcinoma epithelia cells. Oncology Letters, 15, 6622-6628. https://doi.org/10.3892/ol.2018.8111
MLA
Xia, Y., Zhang, X., Bo, A., Sun, J., Li, M."Sodium citrate inhibits the proliferation of human gastric adenocarcinoma epithelia cells". Oncology Letters 15.5 (2018): 6622-6628.
Chicago
Xia, Y., Zhang, X., Bo, A., Sun, J., Li, M."Sodium citrate inhibits the proliferation of human gastric adenocarcinoma epithelia cells". Oncology Letters 15, no. 5 (2018): 6622-6628. https://doi.org/10.3892/ol.2018.8111
Copy and paste a formatted citation
x
Spandidos Publications style
Xia Y, Zhang X, Bo A, Sun J and Li M: Sodium citrate inhibits the proliferation of human gastric adenocarcinoma epithelia cells. Oncol Lett 15: 6622-6628, 2018.
APA
Xia, Y., Zhang, X., Bo, A., Sun, J., & Li, M. (2018). Sodium citrate inhibits the proliferation of human gastric adenocarcinoma epithelia cells. Oncology Letters, 15, 6622-6628. https://doi.org/10.3892/ol.2018.8111
MLA
Xia, Y., Zhang, X., Bo, A., Sun, J., Li, M."Sodium citrate inhibits the proliferation of human gastric adenocarcinoma epithelia cells". Oncology Letters 15.5 (2018): 6622-6628.
Chicago
Xia, Y., Zhang, X., Bo, A., Sun, J., Li, M."Sodium citrate inhibits the proliferation of human gastric adenocarcinoma epithelia cells". Oncology Letters 15, no. 5 (2018): 6622-6628. https://doi.org/10.3892/ol.2018.8111
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team