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Article

LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer

  • Authors:
    • Jang Hee Hong
    • Eun‑Heui Jin
    • Soyeon Kim
    • Kyu‑Sang Song
    • Jae Kyu Sung
  • View Affiliations / Copyright

    Affiliations: Clinical Trials Center, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon 35015, Republic of Korea, Department of Pathology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon 35015, Republic of Korea, Department of Internal Medicine, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon 35015, Republic of Korea
  • Pages: 6666-6670
    |
    Published online on: February 27, 2018
       https://doi.org/10.3892/ol.2018.8121
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Abstract

DNA methylation is an important epigenetic modification that alters gene expression; DNA hypomethylation contributes to tumorigenesis through multiple processes. In the present study, the methylation of long interspersed element-1 (LINE-1) in 95 gastric cancer (GC) tissues and matched adjacent normal tissues was investigated by pyrosequencing. LINE‑1 methylation was compared with the expression of ubiquitin‑like with PHD and ring‑finger protein 1 (UHRF1), an essential regulator of DNA methylation, using reverse transcription‑quantitative polymerase chain reaction. Significant hypomethylation of LINE‑1 and overexpression of UHRF1 were observed in GC tissues compared with the matched controls (P<0.001 and P=0.001, respectively). LINE‑1 hypomethylation was inversely correlated with UHRF1 overexpression in GC tissues (r=‑0.026, P=0.028). In addition, LINE‑1 hypomethylation in GC was significantly associated with Lauren's histological classification, tumor differentiation and background intestinal metaplasia (P=0.014, P=0.042 and P=0.034, respectively). These results suggest that LINE‑1 hypomethylation may be a potential biomarker for GC and it is indirectly regulated by UHRF1 overexpression.
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Copy and paste a formatted citation
Spandidos Publications style
Hong JH, Jin EH, Kim S, Song KS and Sung JK: LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer. Oncol Lett 15: 6666-6670, 2018.
APA
Hong, J.H., Jin, E., Kim, S., Song, K., & Sung, J.K. (2018). LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer. Oncology Letters, 15, 6666-6670. https://doi.org/10.3892/ol.2018.8121
MLA
Hong, J. H., Jin, E., Kim, S., Song, K., Sung, J. K."LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer". Oncology Letters 15.5 (2018): 6666-6670.
Chicago
Hong, J. H., Jin, E., Kim, S., Song, K., Sung, J. K."LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer". Oncology Letters 15, no. 5 (2018): 6666-6670. https://doi.org/10.3892/ol.2018.8121
Copy and paste a formatted citation
x
Spandidos Publications style
Hong JH, Jin EH, Kim S, Song KS and Sung JK: LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer. Oncol Lett 15: 6666-6670, 2018.
APA
Hong, J.H., Jin, E., Kim, S., Song, K., & Sung, J.K. (2018). LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer. Oncology Letters, 15, 6666-6670. https://doi.org/10.3892/ol.2018.8121
MLA
Hong, J. H., Jin, E., Kim, S., Song, K., Sung, J. K."LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer". Oncology Letters 15.5 (2018): 6666-6670.
Chicago
Hong, J. H., Jin, E., Kim, S., Song, K., Sung, J. K."LINE-1 hypomethylation is inversely correlated with UHRF1 overexpression in gastric cancer". Oncology Letters 15, no. 5 (2018): 6666-6670. https://doi.org/10.3892/ol.2018.8121
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