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Article

Dendritic cells loaded with HeLa-derived exosomes simulate an antitumor immune response

  • Authors:
    • Guoping Ren
    • Yanhong Wang
    • Shexia Yuan
    • Baolian Wang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics, Xinxiang Central Hospital, Xinxiang, Henan 453000, P.R. China
  • Pages: 6636-6640
    |
    Published online on: February 27, 2018
       https://doi.org/10.3892/ol.2018.8126
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Abstract

The aim of the present study was to investigate the effect of loading dendritic cells (DCs) with HeLa-derived exosomes on cytotoxic T‑lymphocyte (CTL) responses, and the cytotoxic effects of CTL responses on the HeLa cell line. Ultrafiltration centrifugation combined with sucrose density gradient ultracentrifugation was applied to isolate exosomes (HeLa‑exo) from the supernatant of HeLa cells. Morphological features of HeLa‑exo were identified by transmission electron microscopy (TEM), and the expression of cluster of differentiation (CD)63 was detected by western blotting. Next, monocytes were isolated from peripheral blood and cultured with the removal of adherent cells to induce DC proliferation. DCs were then phenotypically characterized by flow cytometry. Finally, MTT assays were performed to analyze the effects of DCs loaded with HeLa‑exo on T cell proliferation and cytotoxicity assays to evaluate the effect of CTL responses on HeLa cells. TEM revealed that HeLa‑exo exhibit typical cup‑shaped morphology with a diameter range of 30‑100 nm. It was also identified that the CD63 surface antigen is expressed on HeLa‑exo. Furthermore, monocyte‑derived DCs were able to express CD1a, suggesting that DC induction was a success. DCs exhibited hair‑like protrusions and other typical dendritic cell morphology. Furthermore, DCs loaded with HeLa‑exo could enhance CTL proliferation and the cytotoxic activity of CTLs compared with DCs without HeLa‑exo (P<0.05). In conclusion, DCs loaded with HeLa‑exo may promote T cell proliferation and induce CTL responses to inhibit the growth of cervical cancer cells in vitro.
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Copy and paste a formatted citation
Spandidos Publications style
Ren G, Wang Y, Yuan S and Wang B: Dendritic cells loaded with HeLa-derived exosomes simulate an antitumor immune response. Oncol Lett 15: 6636-6640, 2018.
APA
Ren, G., Wang, Y., Yuan, S., & Wang, B. (2018). Dendritic cells loaded with HeLa-derived exosomes simulate an antitumor immune response. Oncology Letters, 15, 6636-6640. https://doi.org/10.3892/ol.2018.8126
MLA
Ren, G., Wang, Y., Yuan, S., Wang, B."Dendritic cells loaded with HeLa-derived exosomes simulate an antitumor immune response". Oncology Letters 15.5 (2018): 6636-6640.
Chicago
Ren, G., Wang, Y., Yuan, S., Wang, B."Dendritic cells loaded with HeLa-derived exosomes simulate an antitumor immune response". Oncology Letters 15, no. 5 (2018): 6636-6640. https://doi.org/10.3892/ol.2018.8126
Copy and paste a formatted citation
x
Spandidos Publications style
Ren G, Wang Y, Yuan S and Wang B: Dendritic cells loaded with HeLa-derived exosomes simulate an antitumor immune response. Oncol Lett 15: 6636-6640, 2018.
APA
Ren, G., Wang, Y., Yuan, S., & Wang, B. (2018). Dendritic cells loaded with HeLa-derived exosomes simulate an antitumor immune response. Oncology Letters, 15, 6636-6640. https://doi.org/10.3892/ol.2018.8126
MLA
Ren, G., Wang, Y., Yuan, S., Wang, B."Dendritic cells loaded with HeLa-derived exosomes simulate an antitumor immune response". Oncology Letters 15.5 (2018): 6636-6640.
Chicago
Ren, G., Wang, Y., Yuan, S., Wang, B."Dendritic cells loaded with HeLa-derived exosomes simulate an antitumor immune response". Oncology Letters 15, no. 5 (2018): 6636-6640. https://doi.org/10.3892/ol.2018.8126
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