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Article

Peroxisome proliferator-activated receptor-γ agonist-mediated inhibition of cell growth is independent of apoptosis in human epidermoid carcinoma A431 cells

  • Authors:
    • Qian Li
    • Yu‑Sheng Peng
    • Ping‑Jiao Chen
    • Meng‑Lei Wang
    • Can Cao
    • Hao Xiong
    • Jing Zhang
    • Ming‑Hua Chen
    • Xue‑Biao Peng
    • Kang Zeng
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
  • Pages: 6578-6584
    |
    Published online on: February 28, 2018
       https://doi.org/10.3892/ol.2018.8136
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Abstract

Evidence suggests that peroxisome proliferator activated receptor‑γ (PPAR‑γ) acts as a tumor suppressor in multiple types of cancer; however, the role of action of PPAR‑γ on human epidermoid carcinoma is unclear. The present study investigated the effects of a PPAR‑γ agonist, rosiglitazone, on human epidermoid carcinoma cell growth using the A431 cell line. The effects of rosiglitazone on cell viability and proliferation were evaluated with MTS and [3H] thymidine incorporation assays. The effects of rosiglitazone on the cell cycle and apoptosis were analyzed by flow cytometry, and western blotting. It was identified that rosiglitazone inhibited A431 cell proliferation in a dose‑dependent manner, increased the proportion of cells in the G1 phase, but did not affect apoptosis. Consistently, there was a significant decrease in the expression of cell proliferation‑associated proteins, including cyclin D1, cyclin‑dependent kinase (Cdk)2 and Cdk4 in A431 cells treated with rosiglitazone. This decrease was rescued by a selective antagonist of PPAR‑γ or specific PPAR‑γ small interfering RNAs. However, the ratio of B‑cell lymphoma 2 (Bcl‑2) to Bcl‑2 associated X protein, which is associated with cell apoptosis, was not affected by these treatments. The data of the present study suggest that the PPAR‑γ agonist rosiglitazone inhibits human epidermoid carcinoma cell growth through regulating the expression of the cell cycle‑associated proteins, and that this effect is independent of apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Li Q, Peng YS, Chen PJ, Wang ML, Cao C, Xiong H, Zhang J, Chen MH, Peng XB, Zeng K, Zeng K, et al: Peroxisome proliferator-activated receptor-γ agonist-mediated inhibition of cell growth is independent of apoptosis in human epidermoid carcinoma A431 cells. Oncol Lett 15: 6578-6584, 2018.
APA
Li, Q., Peng, Y., Chen, P., Wang, M., Cao, C., Xiong, H. ... Zeng, K. (2018). Peroxisome proliferator-activated receptor-γ agonist-mediated inhibition of cell growth is independent of apoptosis in human epidermoid carcinoma A431 cells. Oncology Letters, 15, 6578-6584. https://doi.org/10.3892/ol.2018.8136
MLA
Li, Q., Peng, Y., Chen, P., Wang, M., Cao, C., Xiong, H., Zhang, J., Chen, M., Peng, X., Zeng, K."Peroxisome proliferator-activated receptor-γ agonist-mediated inhibition of cell growth is independent of apoptosis in human epidermoid carcinoma A431 cells". Oncology Letters 15.5 (2018): 6578-6584.
Chicago
Li, Q., Peng, Y., Chen, P., Wang, M., Cao, C., Xiong, H., Zhang, J., Chen, M., Peng, X., Zeng, K."Peroxisome proliferator-activated receptor-γ agonist-mediated inhibition of cell growth is independent of apoptosis in human epidermoid carcinoma A431 cells". Oncology Letters 15, no. 5 (2018): 6578-6584. https://doi.org/10.3892/ol.2018.8136
Copy and paste a formatted citation
x
Spandidos Publications style
Li Q, Peng YS, Chen PJ, Wang ML, Cao C, Xiong H, Zhang J, Chen MH, Peng XB, Zeng K, Zeng K, et al: Peroxisome proliferator-activated receptor-γ agonist-mediated inhibition of cell growth is independent of apoptosis in human epidermoid carcinoma A431 cells. Oncol Lett 15: 6578-6584, 2018.
APA
Li, Q., Peng, Y., Chen, P., Wang, M., Cao, C., Xiong, H. ... Zeng, K. (2018). Peroxisome proliferator-activated receptor-γ agonist-mediated inhibition of cell growth is independent of apoptosis in human epidermoid carcinoma A431 cells. Oncology Letters, 15, 6578-6584. https://doi.org/10.3892/ol.2018.8136
MLA
Li, Q., Peng, Y., Chen, P., Wang, M., Cao, C., Xiong, H., Zhang, J., Chen, M., Peng, X., Zeng, K."Peroxisome proliferator-activated receptor-γ agonist-mediated inhibition of cell growth is independent of apoptosis in human epidermoid carcinoma A431 cells". Oncology Letters 15.5 (2018): 6578-6584.
Chicago
Li, Q., Peng, Y., Chen, P., Wang, M., Cao, C., Xiong, H., Zhang, J., Chen, M., Peng, X., Zeng, K."Peroxisome proliferator-activated receptor-γ agonist-mediated inhibition of cell growth is independent of apoptosis in human epidermoid carcinoma A431 cells". Oncology Letters 15, no. 5 (2018): 6578-6584. https://doi.org/10.3892/ol.2018.8136
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