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Article Open Access

Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway

  • Authors:
    • Ran Liu
    • Ye‑Ning Yang
    • Lan Yi
    • Jing Qing
    • Qing‑Ye Li
    • Wen‑Song Wang
    • Juan Wang
    • Yu‑Xian Tang
    • Hui Tan
  • View Affiliations / Copyright

    Affiliations: Cancer Research Institute, Key Laboratory of Tumor Cellular and Molecular Pathology, College of Hunan Province, University of South China, Hunan 42100, P.R. China, Department of Pathology, The First People's Hospital of Youxian, Youxian, Hunan 412300, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6377-6385
    |
    Published online on: March 1, 2018
       https://doi.org/10.3892/ol.2018.8139
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Abstract

The present study examined the effect of diallyl disulfide (DADS) on the invasion and migration ability of HL‑60 cells with a high expression of parkinsonism associated deglycase (DJ‑1) in the nucleus (HHDN), and its molecular mechanism. A western blot assay was used to measure the effects of DADS and an Src inhibitor on the expression of DJ‑1 and the Src signal pathway in HHDN. The effects of DADS and Src inhibitors on the invasion and migration ability of HHDN was detected using Transwell migration and invasion chamber experiments. The experiments were divided into three groups: A control group (HL‑60 cells), an empty vector group and a high expression group (HHDN cells). Western blot assays revealed that the expression of DJ‑1 in HHDN was inhibited in a time‑dependent manner following treatment with DADS for 24, 48 and 72 h. Following DADS treatment, the expression of phosphorylated Src (p‑Src) and phosphorylated Fak (p‑Fak) were significantly decreased in all groups compared with the untreated groups, however the expression level of Src, Fak and integrin did not change significantly. Western blot analysis results revealed that following treatment with DADS and Src inhibitor, the expression levels of p‑Src and p‑Fak significantly decreased in all three groups compared with untreated groups, whereas the expression levels of Src, Fak and integrin did not change significantly. The expression of DJ‑1 in HHND was inhibited in time‑dependent manner following treatment with DADS and Src inhibitor for 24, 48 and 72 h. Transwell migration and invasion assay results revealed that DADS and Src inhibitors may suppress migration and invasion in leukemic cells, and a combination of the two treatments may result in more efficient suppression. DADS may downregulate DJ‑1‑mediated invasion and migration in leukemic cells through suppressing the Src‑Fak‑Integrin signaling pathway, and the Src inhibitor may enhance the antitumor effect of DADS.
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Copy and paste a formatted citation
Spandidos Publications style
Liu R, Yang YN, Yi L, Qing J, Li QY, Wang WS, Wang J, Tang YX and Tan H: Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway. Oncol Lett 15: 6377-6385, 2018.
APA
Liu, R., Yang, Y., Yi, L., Qing, J., Li, Q., Wang, W. ... Tan, H. (2018). Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway. Oncology Letters, 15, 6377-6385. https://doi.org/10.3892/ol.2018.8139
MLA
Liu, R., Yang, Y., Yi, L., Qing, J., Li, Q., Wang, W., Wang, J., Tang, Y., Tan, H."Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway". Oncology Letters 15.5 (2018): 6377-6385.
Chicago
Liu, R., Yang, Y., Yi, L., Qing, J., Li, Q., Wang, W., Wang, J., Tang, Y., Tan, H."Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway". Oncology Letters 15, no. 5 (2018): 6377-6385. https://doi.org/10.3892/ol.2018.8139
Copy and paste a formatted citation
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Spandidos Publications style
Liu R, Yang YN, Yi L, Qing J, Li QY, Wang WS, Wang J, Tang YX and Tan H: Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway. Oncol Lett 15: 6377-6385, 2018.
APA
Liu, R., Yang, Y., Yi, L., Qing, J., Li, Q., Wang, W. ... Tan, H. (2018). Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway. Oncology Letters, 15, 6377-6385. https://doi.org/10.3892/ol.2018.8139
MLA
Liu, R., Yang, Y., Yi, L., Qing, J., Li, Q., Wang, W., Wang, J., Tang, Y., Tan, H."Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway". Oncology Letters 15.5 (2018): 6377-6385.
Chicago
Liu, R., Yang, Y., Yi, L., Qing, J., Li, Q., Wang, W., Wang, J., Tang, Y., Tan, H."Diallyl disulfide effect on the invasion and migration ability of HL-60 cells with a high expression of DJ-1 in the nucleus through the suppression of the Src signaling pathway". Oncology Letters 15, no. 5 (2018): 6377-6385. https://doi.org/10.3892/ol.2018.8139
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