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Article

Cantharidin suppresses cell growth and migration, and activates autophagy in human non-small cell lung cancer cells

  • Authors:
    • Yan‑Peng Liu
    • Ling Li
    • Liang Xu
    • E‑Nuo Dai
    • Wei‑Da Chen
  • View Affiliations / Copyright

    Affiliations: Department of Internal Medicine, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China, Department of Surgery, Affiliated Hospital of Shandong Academy of Medical Sciences, Jinan, Shandong 250031, P.R. China, Department of Geriatric Medicine, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250014, P.R. China
  • Pages: 6527-6532
    |
    Published online on: March 1, 2018
       https://doi.org/10.3892/ol.2018.8141
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Abstract

Cantharidin (CTD), a component of Mylabris (blister beetle), is a traditional Chinese medicine that exerts an anticancer effect in multiple types of cancer cells. The aim of the present study was to investigate whether CTD exhibited anti‑metastatic and inhibitory cell proliferation effects against human non‑small cell lung cancer (NSCLC) A549 cells, and the possible underlying mechanism by which this occurs. The results of the present study demonstrated that CTD arrested proliferation, suppressed invasion and migration and induced apoptosis in A549 cells in vitro. Alterations of apoptosis‑associated protein levels, including B‑cell lymphoma‑2 (Bcl‑2), Bcl‑associated X (Bax) and active caspase‑3, were detected. Furthermore, the present study demonstrated that CTD activated autophagy through downregulation of p62 expression and upregulation of microtubule‑associated proteins 1A/1B light chain 3B and Beclin‑1 expression. Additionally, western blot analysis identified that CTD inhibited the phosphatidylinositol 3‑kinase (PI3K)/RAC serine/threonine protein kinase (Akt)/mechanistic target of rapamycin (mTOR) signaling pathway in NSCLC, demonstrating that the levels of phosphorylated (p‑)Akt, p‑mTOR, phosphorylated ribosomal p70S6 protein kinase (p‑p70‑S6K) and cyclin D1 were significantly decreased following treatment with CTD. In conclusion, the results of the present study indicated that CTD impeded cell growth and migration by inhibiting PI3K/Akt/mTOR signaling in NSCLC, and promoted autophagy and apoptosis. CTD exhibited anticancer activity against NSCLC in vitro, revealing it as a potential candidate for the treatment of NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Liu YP, Li L, Xu L, Dai EN and Chen WD: Cantharidin suppresses cell growth and migration, and activates autophagy in human non-small cell lung cancer cells. Oncol Lett 15: 6527-6532, 2018.
APA
Liu, Y., Li, L., Xu, L., Dai, E., & Chen, W. (2018). Cantharidin suppresses cell growth and migration, and activates autophagy in human non-small cell lung cancer cells. Oncology Letters, 15, 6527-6532. https://doi.org/10.3892/ol.2018.8141
MLA
Liu, Y., Li, L., Xu, L., Dai, E., Chen, W."Cantharidin suppresses cell growth and migration, and activates autophagy in human non-small cell lung cancer cells". Oncology Letters 15.5 (2018): 6527-6532.
Chicago
Liu, Y., Li, L., Xu, L., Dai, E., Chen, W."Cantharidin suppresses cell growth and migration, and activates autophagy in human non-small cell lung cancer cells". Oncology Letters 15, no. 5 (2018): 6527-6532. https://doi.org/10.3892/ol.2018.8141
Copy and paste a formatted citation
x
Spandidos Publications style
Liu YP, Li L, Xu L, Dai EN and Chen WD: Cantharidin suppresses cell growth and migration, and activates autophagy in human non-small cell lung cancer cells. Oncol Lett 15: 6527-6532, 2018.
APA
Liu, Y., Li, L., Xu, L., Dai, E., & Chen, W. (2018). Cantharidin suppresses cell growth and migration, and activates autophagy in human non-small cell lung cancer cells. Oncology Letters, 15, 6527-6532. https://doi.org/10.3892/ol.2018.8141
MLA
Liu, Y., Li, L., Xu, L., Dai, E., Chen, W."Cantharidin suppresses cell growth and migration, and activates autophagy in human non-small cell lung cancer cells". Oncology Letters 15.5 (2018): 6527-6532.
Chicago
Liu, Y., Li, L., Xu, L., Dai, E., Chen, W."Cantharidin suppresses cell growth and migration, and activates autophagy in human non-small cell lung cancer cells". Oncology Letters 15, no. 5 (2018): 6527-6532. https://doi.org/10.3892/ol.2018.8141
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