Efficacy of extracts of Celastrus orbiculatus in suppressing migration and invasion by inhibiting the EZH2/ROCK1 signaling pathway in human nasopharyngeal carcinoma

Wang,Xuanyi; Huang,Yuxiang; Chen,Yong; Ma,Yong; Yang,Fei; Qian,Yayun; Dai,Xiaojun; Tao,Lede; Wang,Haibo; Guo,Renhua; Liu,Yanqing

doi:10.3892/ol.2018.8149

Efficacy of extracts of Celastrus orbiculatus in suppressing migration and invasion by inhibiting the EZH2/ROCK1 signaling pathway in human nasopharyngeal carcinoma

Authors:
- Xuanyi Wang
- Yuxiang Huang
- Yong Chen
- Yong Ma
- Fei Yang
- Yayun Qian
- Xiaojun Dai
- Lede Tao
- Haibo Wang
- Renhua Guo
- Yanqing Liu
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Affiliations: Department of Rheumatology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225000, P.R. China, Department of Oncology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225000, P.R. China, Department of Function Examination, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225000, P.R. China, School of Medicine, Institute of Traditional Chinese Medicine and Western Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China, Department of Oncology, TCM Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu 225000, P.R. China, Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
Published online on: March 1, 2018 https://doi.org/10.3892/ol.2018.8149
Pages: 6695-6700

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Abstract

Celastrus orbiculatus extract (COE) has been used in folk medicine in China for the treatment of a number of diseases. In the laboratory, COE exhibits a variety of anticancer functions, including inhibition of metastasis. However, the underlying molecular anti‑metastatic mechanism in nasopharyngeal carcinoma (NPC) cells remains unclear. The aim of the present study was to determine whether the anti‑metastatic effect of COE was involved in inhibiting migration and invasion of human NPC cells. In vitro, cell viability and apoptosis of 5‑8F cells were analyzed using an MTS assay and flow cytometry, respectively. Invasion and migration of 5‑8F cells were analyzed using a Transwell assay. Protein and mRNA expression levels of 5‑8F cells were analyzed by western blot analysis and the reverse transcription‑quantitative polymerase chain reaction, respectively. COE significantly decreased cell viability in 5‑8F cells and inhibited enhancer of zeste homolog 2 (EZH2) and Rho‑associated coiled coil‑containing protein kinase 1 (ROCK1) expression at the mRNA and protein levels. Furthermore, COE decreased the migration and invasion of 5‑8F cells in a dose‑dependent manner. The results of the present study suggested that COE prevents migration and invasion by suppressing the EZH2/ROCK1 signaling pathway in NPC cells. On the basis of the results of the present study, COE may be a novel anticancer agent for the treatment of metastasis in NPC.

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