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miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4

  • Authors:
    • Wei Pu
    • Yongming Shang
    • Qiang Shao
    • Xinpeng Yuan
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, The Central Hospital of Zibo, Zibo, Shandong 255000, P.R. China, Department of Dermatology, Zibo Traditional Chinese Medicine Hospital, Zibo, Shandong 255300, P.R. China
    Copyright: © Pu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7111-7117
    |
    Published online on: March 5, 2018
       https://doi.org/10.3892/ol.2018.8172
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Abstract

Previous studies have explored the functions of microRNA (miR)-146a in different types of cancer through mediating different targets. However, the roles of miR‑146a in malignant melanoma (MM) cell migration and invasion remain largely elusive. In the present study, the potential molecular function of miR‑146a in MM was investigated. Reverse transcription‑quantitative polymerase chain reaction was utilized to detect miR‑146a expression in MM tissues and cell lines. A Transwell assay was performed to confirm the ability of migration and invasion. A luciferase assay and biological analysis were used to predict and determine the targets of miR‑146a. The expression of miR‑146a was upregulated in melanoma tissues and cell lines. Clinicopathological analysis indicated that the miR‑146a level was negatively correlated with the progression of melanoma. Abnormal expression of miR‑146a promoted cell migration and invasion in MM cells. Additionally, it was also observed that Mothers against decapentaplegic homolog 4 (SMAD4) was a novel target of miR‑146a in MM. SMAD4 was negatively associated with miR‑146a in MM and abnormal expression of SMAD4 attenuated miR‑146a‑mediated promotion of cell migration and invasion. In conclusion, miR‑146a functioned as an oncogene by directly targeting SMAD4 and it may be a novel diagnostic and therapeutic marker of MM.
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1 

Miller AJ and Mihm MC Jr: Melanoma. N Engl J Med. 355:51–65. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Brose MS, Volpe P, Feldman M, Kumar M, Rishi I, Gerrero R, Einhorn E, Herlyn M, Minna J, Nicholson A, et al: BRAF and RAS mutations in human lung cancer and melanoma. Cancer Res. 62:6997–7000. 2002.PubMed/NCBI

3 

Haass NK, Smalley KS, Li L and Herlyn M: Adhesion, migration and communication in melanocytes and melanoma. Pigment Cell Res. 18:150–159. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Baroudjian B, Pagès C and Lebbè C: Melanoma, from diagnosis to treatment. Rev Infirm. 16–18. 2016.(In French). View Article : Google Scholar : PubMed/NCBI

5 

Barth A, Wanek LA and Morton DL: Prognostic factors in 1,521 melanoma patients with distant metastases. J Am Coll Surg. 181:193–201. 1995.PubMed/NCBI

6 

Di Leva G, Garofalo M and Croce CM: MicroRNAs in cancer. Annu Rev Pathol. 9:287–314. 2014. View Article : Google Scholar : PubMed/NCBI

7 

He L and Hannon GJ: MicroRNAs: Small RNAs with a big role in gene regulation. Nat Rev Genet. 5:522–531. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Ebert MS and Sharp PA: MicroRNA sponges: Progress and possibilities. RNA. 16:2043–2050. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Krol J, Loedige I and Filipowicz W: The widespread regulation of microRNA biogenesis, function and decay. Nat Rev Genet. 11:597–610. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Fang W, Fan Y, Fa Z, Xu J, Yu H, Li P and Gu J: microRNA-625 inhibits tumorigenicity by suppressing proliferation, migration and invasion in malignant melanoma. Oncotarget. 8:13253–13263. 2017.PubMed/NCBI

12 

Brueckner B, Stresemann C, Kuner R, Mund C, Musch T, Meister M, Sultmann H and Lyko F: The human let-7a-3 locus contains an epigenetically regulated microRNA gene with oncogenic function. Cancer Res. 67:1419–1423. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Esquela-Kerscher A and Slack FJ: Oncomirs-microRNAs with a role in cancer. Nat Rev Cancer. 6:259–269. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Miyaki M and Kuroki T: Role of Smad4 (DPC4) inactivation in human cancer. Biochem Biophys Res Commun. 306:799–804. 2003. View Article : Google Scholar : PubMed/NCBI

15 

Yang G and Yang X: Smad4-mediated TGF-beta signaling in tumorigenesis. Int J Biol Sci. 6:1–8. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Zeng Y, Zhu J, Shen D, Qin H, Lei Z, Li W, Liu Z and Huang JA: MicroRNA-205 targets SMAD4 in non-small cell lung cancer and promotes lung cancer cell growth in vitro and in vivo. Oncotarget. 8:30817–30829. 2016.

17 

Yan P, Klingbiel D, Saridaki Z, Ceppa P, Curto M, McKee TA, Roth A, Tejpar S, Delorenzi M, Bosman FT and Fiocca R: Reduced expression of SMAD4 is associated with poor survival in colon cancer. Clin Cancer Res. 22:3037–3047. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Wang LH, Kim SH, Lee JH, Choi YL, Kim YC, Park TS, Hong YC, Wu CF and Shin YK: Inactivation of SMAD4 tumor suppressor gene during gastric carcinoma progression. Clin Cancer Res. 13:102–110. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Stuelten CH, Buck MB, Dippon J, Roberts AB, Fritz P and Knabbe C: Smad4-expression is decreased in breast cancer tissues: A retrospective study. BMC Cancer. 6:252006. View Article : Google Scholar : PubMed/NCBI

20 

Ding Z, Wu CJ, Chu GC, Xiao Y, Ho D, Zhang J, Perry SR, Labrot ES, Wu X, Lis R, et al: SMAD4-dependent barrier constrains prostate cancer growth and metastatic progression. Nature. 470:269–273. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Meng X, Zhao Y, Wang J, Gao Z, Geng Q and Liu X: Regulatory roles of miRNA-758 and matrix extracellular phosphoglycoprotein in cervical cancer. Exp Ther Med. 14:2789–2794. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Wei YT, Guo DW, Hou XZ and Jiang DQ: miRNA-223 suppresses FOXO1 and functions as a potential tumor marker in breast cancer. Cell Mol Biol (Noisy-le-grand). 63:113–118. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Zu Y, Yang Y, Zhu J, Bo X, Hou S, Zhang B, Qiu J and Zheng J: miR-146a suppresses hepatocellular carcinoma by downregulating TRAF6. Am J Cancer Res. 6:2502–2513. 2016.PubMed/NCBI

25 

Liu Q, Wang W, Yang X, Zhao D, Li F and Wang H: MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer. Oncol Rep. 36:189–196. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Lu D, Yao Q, Zhan C, Le-Meng Z, Liu H, Cai Y, Tu C, Li X, Zou Y and Zhang S: MicroRNA-146a promote cell migration and invasion in human colorectal cancer via carboxypeptidase M/src-FAK pathway. Oncotarget. 8:22674–22684. 2017.PubMed/NCBI

27 

Hung PS, Liu CJ, Chou CS, Kao SY, Yang CC, Chang KW, Chiu TH and Lin SC: miR-146a enhances the oncogenicity of oral carcinoma by concomitant targeting of the IRAK1, TRAF6 and NUMB genes. PLoS One. 8:e799262013. View Article : Google Scholar : PubMed/NCBI

28 

Zhang X, Cao J, Pei Y, Zhang J and Wang Q: Smad4 inhibits cell migration via suppression of JNK activity in human pancreatic carcinoma PANC-1 cells. Oncol Lett. 11:3465–3470. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Xiao B, Zhu ED, Li N, Lu DS, Li W, Li BS, Zhao YL, Mao XH, Guo G, Yu PW and Zou QM: Increased miR-146a in gastric cancer directly targets SMAD4 and is involved in modulating cell proliferation and apoptosis. Oncol Rep. 27:559–566. 2012.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Pu W, Shang Y, Shao Q and Yuan X: miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4. Oncol Lett 15: 7111-7117, 2018.
APA
Pu, W., Shang, Y., Shao, Q., & Yuan, X. (2018). miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4. Oncology Letters, 15, 7111-7117. https://doi.org/10.3892/ol.2018.8172
MLA
Pu, W., Shang, Y., Shao, Q., Yuan, X."miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4". Oncology Letters 15.5 (2018): 7111-7117.
Chicago
Pu, W., Shang, Y., Shao, Q., Yuan, X."miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4". Oncology Letters 15, no. 5 (2018): 7111-7117. https://doi.org/10.3892/ol.2018.8172
Copy and paste a formatted citation
x
Spandidos Publications style
Pu W, Shang Y, Shao Q and Yuan X: miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4. Oncol Lett 15: 7111-7117, 2018.
APA
Pu, W., Shang, Y., Shao, Q., & Yuan, X. (2018). miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4. Oncology Letters, 15, 7111-7117. https://doi.org/10.3892/ol.2018.8172
MLA
Pu, W., Shang, Y., Shao, Q., Yuan, X."miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4". Oncology Letters 15.5 (2018): 7111-7117.
Chicago
Pu, W., Shang, Y., Shao, Q., Yuan, X."miR-146a promotes cell migration and invasion in melanoma by directly targeting SMAD4". Oncology Letters 15, no. 5 (2018): 7111-7117. https://doi.org/10.3892/ol.2018.8172
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