Tetrandrine suppresses lung cancer growth and induces apoptosis, potentially via the VEGF/HIF-1α/ICAM-1 signaling pathway

  • Authors:
    • Zhuo Chen
    • Liang Zhao
    • Feng Zhao
    • Guanghai Yang
    • Jian Jun Wang
  • View Affiliations

  • Published online on: March 7, 2018     https://doi.org/10.3892/ol.2018.8190
  • Pages: 7433-7437
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study investigated the effect of tetrandrine on lung cancer cell growth and apoptosis, and its possible underlying molecular mechanism. A549 human lung cancer cells were incubated with between 2.5 and 10 µM tetrandrine for 12, 24 and 48 h, following which the effect of tetrandrine on cell viability and apoptosis were assessed using an MTT assay and flow cytometry. ELISA and western blotting were used to analyze VEGF activity, and the expression of poly (ADP‑ribose) polymerase (PARP), phosphorylated protein kinase B (Akt), Bcl‑2‑associated X protein (Bax), hypoxia inducible factor (HIF)‑1α and inter‑cellular adhesion molecule‑1 (ICAM‑1). Tetrandrine effectively suppressed the growth of and induced apoptosis in A549 lung cancer cells. The expression of PARP, Bax, intercellular adhesion molecule‑1 (ICAM‑1) and vascular endothelial growth factor (VEGF) was significantly upregulated, and the phosphorylation of Akt and expression of HIF‑1α was significantly suppressed in A549 lung cancer cells. Therefore, tetrandrine may suppress cell viability and induce apoptosis via the VEGF/HIF‑1α/ICAM‑1 signaling pathway.
View Figures
View References

Related Articles

Journal Cover

May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chen Z, Zhao L, Zhao F, Yang G and Wang JJ: Tetrandrine suppresses lung cancer growth and induces apoptosis, potentially via the VEGF/HIF-1α/ICAM-1 signaling pathway. Oncol Lett 15: 7433-7437, 2018
APA
Chen, Z., Zhao, L., Zhao, F., Yang, G., & Wang, J.J. (2018). Tetrandrine suppresses lung cancer growth and induces apoptosis, potentially via the VEGF/HIF-1α/ICAM-1 signaling pathway. Oncology Letters, 15, 7433-7437. https://doi.org/10.3892/ol.2018.8190
MLA
Chen, Z., Zhao, L., Zhao, F., Yang, G., Wang, J. J."Tetrandrine suppresses lung cancer growth and induces apoptosis, potentially via the VEGF/HIF-1α/ICAM-1 signaling pathway". Oncology Letters 15.5 (2018): 7433-7437.
Chicago
Chen, Z., Zhao, L., Zhao, F., Yang, G., Wang, J. J."Tetrandrine suppresses lung cancer growth and induces apoptosis, potentially via the VEGF/HIF-1α/ICAM-1 signaling pathway". Oncology Letters 15, no. 5 (2018): 7433-7437. https://doi.org/10.3892/ol.2018.8190