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Article

Integration sites of human papillomavirus 18 in esophageal cancer samples

  • Authors:
    • Shuying Li
    • Zhanjun Liu
    • Jianghong Yan
    • Shangbo Sun
    • Xiaoli Hou
    • Dianqing Liu
    • Ke Zhang
    • Jin‑Tao Li
  • View Affiliations / Copyright

    Affiliations: School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China, College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, P.R. China
  • Pages: 7438-7442
    |
    Published online on: March 7, 2018
       https://doi.org/10.3892/ol.2018.8191
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Abstract

To investigate the association between human papillomavirus (HPV) infection and esophageal cancer, genomic DNA was isolated from 189 samples obtained from patients with esophageal carcinoma, and HPV DNA was identified using the polymerase chain reaction (PCR) with the following specific primers: My09/11 for HPV L1 and HPV18 E6 for HPV18. The HPV18 gene products were sequenced to identify the HPV genotype and the HPV18 integration site was verified using PCR amplification of papillomavirus oncogene transcripts. HPV18 oncogene transcript products were ligated into a pMD‑18T plasmid vector and sequenced to confirm the physical location of HPV18 integration. Of the 189 samples, 168 were positive for HPV, of which 33 were positive for HPV18. The sequencing analysis identified two HPV18 E6‑positive samples containing one mutation and two samples containing two mutations in the viral DNA. In total ~600 bp of the HPV18 oncogene transcript was detected in three esophageal cancer samples. Sequence analysis revealed that, in two patients, the HPV18 infection was integrated into human chromosome 5, whereas in the remaining sample the virus was integrated into human chromosome 2. The high prevalence of HPV18 infection suggested that HPV18 infection is a pathogenic factor in esophageal carcinoma progression. The integration of HPV18 DNA into the host cell genome suggests that persistent HPV infection has a role in esophageal epithelial cell malignant transformation and carcinogenesis.
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1 

Zhao J, He YT, Zheng RS, Zhang SW and Chen WQ: Analysis of esophageal cancer time trends in China, 1989–2008. Asian Pac J Cancer Prev. 13:4613–4617. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Sun X, Chen W, Chen Z, Wen D, Zhao D and He Y: Population-based casecontrol study on risk factors for esophageal cancer in five high-risk areas in China. Asian Pac J Cancer Prev. 11:1631–1636. 2010.PubMed/NCBI

3 

Zhang HZ, Jin GF and Shen HB: Epidemiologic differences in esophageal cancer between Asian and Western populations. Chin J Cancer. 31:281–286. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Esophageal cancer: Epidemiology, pathogenesis and prevention. Nat Clin Pract Gastroenterol Hepatol. 5:517–526. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Syrjänen KJ: Histological changes identical to those of condylomatous lesions found in esophageal squamous cell carcinomas. Arch Geschwulstforsch. 52:283–292. 1982.PubMed/NCBI

6 

Dong HC, Cui XB, Wang LH, Li M, Shen YY, Zhu JB, Li CF, Hu JM, Li SG, Yang L, et al: Type-specific detection of human papillomaviruses in Kazakh esophageal squamous cell carcinoma by genotyping both E6 and L1 genes with MALDI-TOF mass spectrometry. Int J Clin Exp Pathol. 8:13156–13165. 2015.PubMed/NCBI

7 

Türkay DÖ, Vural Ç, Sayan M and Gürbüz Y: Detection of human papillomavirus in esophageal and gastroesophageal junction tumors: A retrospective study by real-time polymerase chain reaction in an instutional experience from Turkey and review of literature. Pathol Res Pract. 212:77–82. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Ludmir EB, Stephens SJ, Palta M, Willett CG and Czito BG: Human papillomavirus tumor infection in esophageal squamous cell carcinoma. J Gastrointest Oncol. 6:287–295. 2015.PubMed/NCBI

9 

Georgantis G, Syrakos T, Agorastos T, Miliaras S, Gagalis A, Tsoulfas G, Spanos K and Marakis G: Detection of human papillomavirus DNA in esophageal carcinoma in Greece. World J Gastroenterol. 21:2352–2357. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Liu HY, Zhou SL, Ku JW, Zhang DY, Li B, Han XN, Fan ZM, Cui JL, Lin HL, Guo ET, et al: Prevalence of human papillomavirus infection in esophageal and cervical cancers in the high incidence area for the two diseases from 2007 to 2009 in Linzhou of Henan Province, Northern China. Arch Virol. 159:1393–1401. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Zhang K, Li JT, Li SY, Zhu LH, Zhou L and Zeng Y: Integration of human papillomavirus 18 DNA in esophageal carcinoma 109 cells. World J Gastroenterol. 17:4242–4246. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Zhang R, Shen C, Zhao L, Wang J, McCrae M, Chen X and Lu F: Dysregulation of host cellular genes targeted by human papillomavirus (HPV) integration contributes to HPV-related cervical carcinogenesis. Int J Cancer. 138:1163–1174. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Xu F, Cao M, Shi Q, Chen H, Wang Y and Li X: Integration of the full-length HPV16 genome in cervical cancer and Caski and Siha cell lines and the possible ways of HPV integration. Virus Genes. 50:210–220. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Klaes R, Woerner SM, Ridder R, Wentzensen N, Duerst M, Schneider A, Lotz B, Melsheimer P and von Knebel Doeberitz M: Detection of high-risk cervical intraepithelial neoplasia and cervical cancer by amplification of transcripts derived from integrated papillomavirus oncogenes. Cancer Res. 59:6132–6136. 1999.PubMed/NCBI

15 

Reeh M, Nentwich MF, von Loga K, Schade J, Uzunoglu FG, Koenig AM, Bockhorn M, Rosch T, Izbicki JR and Bogoevski D: An attempt at validation of the Seventh edition of the classification by the International UnionAgainst Cancer for esophageal carcinoma. Ann Thorac Surg. 93:890–896. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Lee DC, Cheung CY, Law AH, Mok CK, Peiris M and Lau AS: p38 mitogen- activated protein kinase-dependent hyperinduction of tumor necrosis factor alpha expression in response to avian influenza virus H5N1. J Virol. 79:10147–10154. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Karlsen F, Kalantari M, Jenkins A, Pettersen E, Kristensen G, Holm R, Johansson B and Hagmar B: Use of multiple PCR sets for optimal detection of human papillomavirus. J Clin Microbiol. 34:2095–2100. 1996.PubMed/NCBI

18 

Syrjänen KJ: HPV infections and oesophageal cancer. J Clin Pathol. 55:721–728. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Kadaja M, Sumerina A, Verst T, Ojarand M, Ustav E and Ustav M: Genomic instability of the host cell induced by the human papillomavirus replication machinery. EMBO J. 26:2180–2191. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Johansson C, Somberg M, Li X, Winquist Backström E, Fay J, Ryan F, Pim D, Banks L and Schwartz S: HPV-16 E2 contributes to induction of HPV-16 late gene expression by inhibiting early polyadenylation. EMBO J. 31:3212–3227. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Venuti A and Marcante ML: Presence of human papillomavirus type 18 DNA in vulvar carcinomas and its integration into the cell genome. J Gen Virol. 70:1587–1592. 1989. View Article : Google Scholar : PubMed/NCBI

22 

Klimov E, Vinokourova S, Moisjak E, Rakhmanaliev E, Kobseva V, Laimins L, Kisseljov F and Sulimova G: Human papilloma viruses and cervical tumours: Mapping of integration sites and analysis of adjacent cell ularsequences. BMC Cancer. 2:242002. View Article : Google Scholar : PubMed/NCBI

23 

Akagi K, Li J, Broutian TR, Padilla-Nash H, Xiao W, Jiang B, Rocco JW, Teknos TN, Kumar B, Wangsa D, et al: Genome-wide analysis of HPV integration in human cancers reveals recurrent, focal genomic instability. Genome Res. 24:185–199. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Xu B, Chotewutmontri S, Wolf S, Klos U, Schmitz M, Dürst M and Schwarz E: Multiplex identification of human papillomavirus 16 DNA integration sites in cervical carcinomas. PLoS One. 8:e666932013. View Article : Google Scholar : PubMed/NCBI

25 

Lu X, Lin Q, Lin M, Duan P, Ye L, Chen J, Chen X, Zhang L and Xue X: Multiple-integrations of HPV16 genome and altered transcription of viral oncogenes and cellular genes are associated with the development of cervical cancer. PLoS One. 9:e975882014. View Article : Google Scholar : PubMed/NCBI

26 

Steger G and Corbach S: Dose-dependent regulation of the early promoter of human papillomavirus type 18 by the viral E2 protein. J Virol. 71:50–58. 1997.PubMed/NCBI

27 

Nishimura A, Ono T, Ishimoto A, Dowhanick JJ, Frizzell MA, Howley PM and Sakai H: Mechanisms of human papillomavirus E2-mediated repression of viral oncogene expression and cervical cancer cell growth inhibition. J Virol. 74:3752–3760. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Wells SI, Aronow BJ, Wise TM, Williams SS, Couget JA and Howley PM: Transcriptome signature of irreversible senescence in human papillomavirus-positive cervical cancer cells. Proc Natl Acad Sci USA. 100:7093–7098. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Bergner S, Halec G, Schmitt M, Aubin F, Alonso A and Auvinen E: Individual and complementary effects of human papillomavirus oncogenes on epithelial cell proliferation and differentiation. Cells Tissues Organs. 201:97–108. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Ekalaksananan T, Jungpol W, Prasitthimay C, Wongjampa W, Kongyingyoes B and Pientong C: Polymorphisms and functional analysis of the intact human papillomavirus16 e2 gene. Asian Pac J Cancer Prev. 15:10255–10262. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Scheffner M, Romanczuk H, Münger K, Huibregtse JM, Mietz JA and Howley PM: Functions of human papillomavirus proteins. Curr Top Microbiol Immunol. 186:83–99. 1994.PubMed/NCBI

32 

Alp Avcı G: Genomic organization and proteins of human papillomavirus. Mikrobiyol Bul. 46:507–515. 2012.(In Turkish). PubMed/NCBI

33 

Kessis TD, Connolly DC, Hedrick L and Cho KR: Expression of HPV16 E6 or E7 increases integration of foreign DNA. Oncogene. 13:427–431. 1996.PubMed/NCBI

34 

Hillemanns P and Wang X: Integration of HPV-16 and HPV-18 DNA in vulvar intraepithelial neoplasia. Gynecol Oncol. 100:276–282. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Li S, Liu Z, Yan J, Sun S, Hou X, Liu D, Zhang K and Li JT: Integration sites of human papillomavirus 18 in esophageal cancer samples. Oncol Lett 15: 7438-7442, 2018.
APA
Li, S., Liu, Z., Yan, J., Sun, S., Hou, X., Liu, D. ... Li, J. (2018). Integration sites of human papillomavirus 18 in esophageal cancer samples. Oncology Letters, 15, 7438-7442. https://doi.org/10.3892/ol.2018.8191
MLA
Li, S., Liu, Z., Yan, J., Sun, S., Hou, X., Liu, D., Zhang, K., Li, J."Integration sites of human papillomavirus 18 in esophageal cancer samples". Oncology Letters 15.5 (2018): 7438-7442.
Chicago
Li, S., Liu, Z., Yan, J., Sun, S., Hou, X., Liu, D., Zhang, K., Li, J."Integration sites of human papillomavirus 18 in esophageal cancer samples". Oncology Letters 15, no. 5 (2018): 7438-7442. https://doi.org/10.3892/ol.2018.8191
Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Liu Z, Yan J, Sun S, Hou X, Liu D, Zhang K and Li JT: Integration sites of human papillomavirus 18 in esophageal cancer samples. Oncol Lett 15: 7438-7442, 2018.
APA
Li, S., Liu, Z., Yan, J., Sun, S., Hou, X., Liu, D. ... Li, J. (2018). Integration sites of human papillomavirus 18 in esophageal cancer samples. Oncology Letters, 15, 7438-7442. https://doi.org/10.3892/ol.2018.8191
MLA
Li, S., Liu, Z., Yan, J., Sun, S., Hou, X., Liu, D., Zhang, K., Li, J."Integration sites of human papillomavirus 18 in esophageal cancer samples". Oncology Letters 15.5 (2018): 7438-7442.
Chicago
Li, S., Liu, Z., Yan, J., Sun, S., Hou, X., Liu, D., Zhang, K., Li, J."Integration sites of human papillomavirus 18 in esophageal cancer samples". Oncology Letters 15, no. 5 (2018): 7438-7442. https://doi.org/10.3892/ol.2018.8191
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