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Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression

  • Authors:
    • Jing Chen
    • Jun Liang
    • Shihai Liu
    • Shanai Song
    • Wenxuan Guo
    • Fangzhen Shen
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China, Department of Oncology, International Cancer Hospital of Beijing University, Beijing 100000, P.R. China, Central Laboratory, Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6857-6864
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    Published online on: March 7, 2018
       https://doi.org/10.3892/ol.2018.8193
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Abstract

The RAC serine/threonine-protein kinase (AKT) family of serine/threonine protein kinases, particularly the AKT1 isoform, has been identified abnormally expressed in hepatocellular carcinoma (HCC) cells, and is highly associated with cell behavior, including proliferation, survival, metabolism, and tumorigenesis. However, the specific mechanism by which AKT1 elicits these effects requires further study. The purpose of the present study was to reveal the effects of AKT1 on the survival and proliferation of HCC cells, and to investigate the mechanisms involved. Western blotting and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) to evaluate the expression levels of AKT1 in HCC SMMC‑7721 cell line. Molecular mechanisms and the influences of different regulation the expression of AKT1 on HCC cell growth, proliferation were determined by western blotting, MTT and colony formation assays, cell cycle and apoptosis were investigated by flow cytometry. The activation of AKT1 suppressed the expression of phosphatase and tensin homolog and increased the activation of Notch1. The inhibition of AKT1 effectively suppressed the expression of Notch1. Furthermore, the data of the present study indicated that B‑cell lymphoma 2 and cyclin D1 is involved in the regulation of AKT1 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Chen J, Liang J, Liu S, Song S, Guo W and Shen F: Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression. Oncol Lett 15: 6857-6864, 2018.
APA
Chen, J., Liang, J., Liu, S., Song, S., Guo, W., & Shen, F. (2018). Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression. Oncology Letters, 15, 6857-6864. https://doi.org/10.3892/ol.2018.8193
MLA
Chen, J., Liang, J., Liu, S., Song, S., Guo, W., Shen, F."Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression". Oncology Letters 15.5 (2018): 6857-6864.
Chicago
Chen, J., Liang, J., Liu, S., Song, S., Guo, W., Shen, F."Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression". Oncology Letters 15, no. 5 (2018): 6857-6864. https://doi.org/10.3892/ol.2018.8193
Copy and paste a formatted citation
x
Spandidos Publications style
Chen J, Liang J, Liu S, Song S, Guo W and Shen F: Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression. Oncol Lett 15: 6857-6864, 2018.
APA
Chen, J., Liang, J., Liu, S., Song, S., Guo, W., & Shen, F. (2018). Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression. Oncology Letters, 15, 6857-6864. https://doi.org/10.3892/ol.2018.8193
MLA
Chen, J., Liang, J., Liu, S., Song, S., Guo, W., Shen, F."Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression". Oncology Letters 15.5 (2018): 6857-6864.
Chicago
Chen, J., Liang, J., Liu, S., Song, S., Guo, W., Shen, F."Differential regulation of AKT1 contributes to survival and proliferation in hepatocellular carcinoma cells by mediating Notch1 expression". Oncology Letters 15, no. 5 (2018): 6857-6864. https://doi.org/10.3892/ol.2018.8193
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