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Article Open Access

LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway

  • Authors:
    • Hongpeng Zhao
    • Bo Liu
    • Jie Li
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, Shandong Qianfoshan Hospital, Jinan, Shandong 250014, P.R. China, Department of Hepatobiliary Surgery, Shandong Qianfoshan Hospital, Jinan, Shandong 250014, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6839-6844
    |
    Published online on: March 9, 2018
       https://doi.org/10.3892/ol.2018.8222
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Abstract

LIM and SH3 protein 1 (Lasp-1), a focal adhesion protein, serves a critical role in the regulation of cell proliferation and migration. The role of Lasp‑1, as well as the intracellular signaling pathways involved in metastasis and progression of colorectal carcinoma, remains unclear. In the present study, the regulatory effect of Lasp‑1 and the underlying molecular mechanism involved in migration and invasion of colorectal carcinoma were investigated. RNA interference and overexpression in SW480 cells were performed to elucidate the role of Lasp‑1. Reverse transcription-quantitative polymerase chain reaction, western blotting and immunofluorescence were conducted to determine the mRNA and protein levels of Lasp‑1 and extracellular‑signal‑regulated kinase 1/2 (ERK1/2) in SW480 cells as well as tumor and adjacent normal tissues obtained from 20 patients with colorectal carcinoma. Furthermore, a cell proliferation assay, flow cytometric analysis, and cell migration and invasion assays were performed to examine the effect of Lasp‑1 on cell growth. The results of the present study demonstrated that Lasp‑1 and ERK1/2 were upregulated in tumor tissue compared with adjacent normal colorectal mucosa. Cell‑based in vitro assays demonstrated that Lasp‑1 knockdown suppressed the expression and activation of ERK1/2, whereas Lasp‑1 overexpression resulted in ERK1/2 upregulation. Additionally, Lasp‑1 knockdown inhibited cell proliferation, migration, and invasion and induced cellular apoptosis and G0/G1 cell‑cycle arrest. The results of the present study indicate that Lasp‑1 serves a critical role in the progression of colorectal carcinoma regulating the activation of the mitogen-activated protein kinase signaling pathway.
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1 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Quintero E, Carrillo M, Leoz ML, Cubiella J, Gargallo C, Lanas A, Bujanda L, Gimeno-García AZ, Hernández-Guerra M, Nicolás-Pérez D, et al: Risk of advanced neoplasia in first-degree relatives with colorectal cancer: A large multicenter cross-sectional study. PLoS Med. 13:e10020082016. View Article : Google Scholar : PubMed/NCBI

4 

Krbal L, Hanušová V, Soukup J, John S, Matoušková P and Ryška A: Contribution of in vitro comparison of colorectal carcinoma cells from primary and metastatic lesions to elucidation of mechanisms of tumor progression and response to anticancer therapy. Tumour Biol. 37:9565–9578. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Tan WJ, Chew MH, Tan IB, Law JH, Zhao R, Acharyya S, Mao YL, Fernandez LG, Loi CT and Tang CL: Palliative surgical intervention in metastatic colorectal carcinoma: A prospective analysis of quality of life. Colorectal Dis. 18:357–363. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Grunewald TG, Kammerer U, Schulze E, Schindler D, Honig A, Zimmer M and Butt E: Silencing of LASP-1 influences zyxin localization, inhibits proliferation and reduces migration in breast cancer cells. Exp Cell Res. 312:974–982. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Vaman VSA, Poppe H, Houben R, Grunewald TG, Goebeler M and Butt E: LASP1, a newly identified melanocytic protein with a possible role in melanin release, but not in melanoma progression. PLoS One. 10:e01292192015. View Article : Google Scholar : PubMed/NCBI

8 

Yang F, Zhou X, Du S, Zhao Y, Ren W, Deng Q, Wang F and Yuan J: LIM and SH3 domain protein 1 (LASP-1) overexpression was associated with aggressive phenotype and poor prognosis in clear cell renal cell cancer. PLoS One. 9:e1005572014. View Article : Google Scholar : PubMed/NCBI

9 

Zheng J, Wang F, Lu S and Wang X: LASP-1, regulated by miR-203, promotes tumor proliferation and aggressiveness in human non-small cell lung cancer. Exp Mol Pathol. 100:116–124. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Hailer A, Grunewald TG, Orth M, Reiss C, Kneitz B, Spahn M and Butt E: Loss of tumor suppressor mir-203 mediates overexpression of LIM and SH3 protein 1 (LASP1) in high-risk prostate cancer thereby increasing cell proliferation and migration. Oncotarget. 5:4144–4153. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Li Z, Chen Y, Wang X, Zhang H, Zhang Y, Gao Y, Weng M, Wang L, Liang H, Li M, et al: LASP-1 induces proliferation, metastasis and cell cycle arrest at the G2/M phase in gallbladder cancer by down-regulating S100P via the PI3K/AKT pathway. Cancer Lett. 372:239–250. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Payton S: Bladder cancer: LASP-1-a promising urine marker for detection of bladder cancer. Nat Rev Urol. 9:2402012. View Article : Google Scholar : PubMed/NCBI

13 

Ardelt P, Grünemay N, Strehl A, Jilg C, Miernik A, Kneitz B and Butt E: LASP-1, a novel urinary marker for detection of bladder cancer. Urol Oncol. 31:1591–1598. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Orth MF, Cazes A, Butt E and Grunewald TG: An update on the LIM and SH3 domain protein 1 (LASP1): A versatile structural, signaling, and biomarker protein. Oncotarget. 6:26–42. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Salvi A, Bongarzone I, Ferrari L, Abeni E, Arici B, De Bortoli M, Scuri S, Bonini D, Grossi I, Benetti A, et al: Molecular characterization of LASP-1 expression reveals vimentin as its new partner in human hepatocellular carcinoma cells. Int J Oncol. 46:1901–1912. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Wang H, Li W, Jin X, Cui S and Zhao L: LIM and SH3 protein 1, a promoter of cell proliferation and migration, is a novel independent prognostic indicator in hepatocellular carcinoma. Eur J Cancer. 49:974–983. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Zhao L, Wang H, Liu C, Liu Y, Wang X, Wang S, Sun X, Li J, Deng Y, Jiang Y and Ding Y: Promotion of colorectal cancer growth and metastasis by the LIM and SH3 domain protein 1. Gut. 59:1226–1235. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Zhao L, Wang H, Sun X and Ding Y: Comparative proteomic analysis identifies proteins associated with the development and progression of colorectal carcinoma. FEBS J. 277:4195–4204. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Zhang H, Li Z, Chu B, Zhang F, Zhang Y, Ke F, Chen Y, Xu Y, Liu S, Zhao S, et al: Upregulated LASP-1 correlates with a malignant phenotype and its potential therapeutic role in human cholangiocarcinoma. Tumour Biol. 37:8305–8315. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Wang H, Shi J, Luo Y, Liao Q, Niu Y, Zhang F, Shao Z, Ding Y and Zhao L: LIM and SH3 protein 1 induces TGFβ-mediated epithelial-mesenchymal transition in human colorectal cancer by regulating S100A4 expression. Clin Cancer Res. 20:5835–5847. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Lin YH, Park ZY, Lin D, Brahmbhatt AA, Rio MC, Yates JR III and Klemke RL: Regulation of cell migration and survival by focal adhesion targeting of Lasp-1. J Cell Biol. 165:421–432. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Wang H, An H, Wang B, Liao Q, Li W, Jin X, Cui S, Zhang Y, Ding Y and Zhao L: miR-133a represses tumour growth and metastasis in colorectal cancer by targeting LIM and SH3 protein 1 and inhibiting the MAPK pathway. Eur J Cancer. 49:3924–3935. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Xu L, Zhang Y, Wang H, Zhang G, Ding Y and Zhao L: Tumor suppressor miR-1 restrains epithelial-mesenchymal transition and metastasis of colorectal carcinoma via the MAPK and PI3K/AKT pathway. J Transl Med. 12:2442014. View Article : Google Scholar : PubMed/NCBI

24 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Tomasetto C, Moog-Lutz C, Régnier CH, Schreiber V, Basset P and Rio MC: Lasp-1 (MLN 50) defines a new LIM protein subfamily characterized by the association of LIM and SH3 domains. FEBS Lett. 373:245–249. 1995. View Article : Google Scholar : PubMed/NCBI

26 

Kay BK: SH3 domains come of age. FEBS Lett. 586:2606–2608. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Hammarström A, Berndt KD, Sillard R, Adermann K and Otting G: Solution structure of a naturally-occurring zinc-peptide complex demonstrates that the N-terminal zinc-binding module of the Lasp-1 LIM domain is an independent folding unit. Biochemistry. 35:12723–12732. 1996. View Article : Google Scholar : PubMed/NCBI

28 

Pappas CT, Bliss KT, Zieseniss A and Gregorio CC: The Nebulin family: An actin support group. Trends Cell Biol. 21:29–37. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Mihlan S, Reiß C, Thalheimer P, Herterich S, Gaetzner S, Kremerskothen J, Pavenstädt HJ, Lewandrowski U, Sickmann A and Butt E: Nuclear import of LASP-1 is regulated by phosphorylation and dynamic protein-protein interactions. Oncogene. 32:2107–2113. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Raman D, Sai J, Neel NF, Chew CS and Richmond A: LIM and SH3 protein-1 modulates CXCR2-mediated cell migration. PLoS One. 5:e100502010. View Article : Google Scholar : PubMed/NCBI

31 

Celeghini C, Voltan R, Rimondi E, Gattei V and Zauli G: Perifosine selectively induces cell cycle block and modulates retinoblastoma and E2F1 protein levels in p53 mutated leukemic cell lines. Invest New Drugs. 29:392–395. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Park SJ, Yang SW and Kim BC: Transforming growth factor-β1 induces cell cycle arrest by activating atypical cyclin-dependent kinase 5 through up-regulation of Smad3-dependent p35 expression in human MCF10A mammary epithelial cells. Biochem Biophys Res Commun. 472:502–507. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Chaudhary P, Sharma R, Sahu M, Vishwanatha JK, Awasthi S and Awasthi YC: 4-Hydroxynonenal induces G2/M phase cell cycle arrest by activation of the ataxia telangiectasia mutated and Rad3-related protein (ATR)/checkpoint kinase 1 (Chk1) signaling pathway. J Biol Chem. 288:20532–20546. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhao H, Liu B and Li J: LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway. Oncol Lett 15: 6839-6844, 2018.
APA
Zhao, H., Liu, B., & Li, J. (2018). LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway. Oncology Letters, 15, 6839-6844. https://doi.org/10.3892/ol.2018.8222
MLA
Zhao, H., Liu, B., Li, J."LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway". Oncology Letters 15.5 (2018): 6839-6844.
Chicago
Zhao, H., Liu, B., Li, J."LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway". Oncology Letters 15, no. 5 (2018): 6839-6844. https://doi.org/10.3892/ol.2018.8222
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao H, Liu B and Li J: LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway. Oncol Lett 15: 6839-6844, 2018.
APA
Zhao, H., Liu, B., & Li, J. (2018). LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway. Oncology Letters, 15, 6839-6844. https://doi.org/10.3892/ol.2018.8222
MLA
Zhao, H., Liu, B., Li, J."LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway". Oncology Letters 15.5 (2018): 6839-6844.
Chicago
Zhao, H., Liu, B., Li, J."LIM and SH3 protein 1 knockdown suppresses proliferation and metastasis of colorectal carcinoma cells via inhibition of the mitogen-activated protein kinase signaling pathway". Oncology Letters 15, no. 5 (2018): 6839-6844. https://doi.org/10.3892/ol.2018.8222
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