Solanine induced apoptosis and increased chemosensitivity to Adriamycin in T-cell acute lymphoblastic leukemia cells

  • Authors:
    • Ying‑Jie Yi
    • Xiu‑Hong Jia
    • Jian‑Yong Wang
    • Jie‑Ru Chen
    • Hong Wang
    • You‑Jie Li
  • View Affiliations

  • Published online on: March 12, 2018     https://doi.org/10.3892/ol.2018.8229
  • Pages: 7383-7388
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Abstract

Solanine is an alkaloid and is the main extract of the traditional Chinese herb, Solanum nigrum Linn. It has been reported that Solanine has anti-inflammatory and antitumor properties. The present study aimed to investigate the antitumor effect of Solanine in Jurkat cells and demonstrate the molecular mechanism of antitumor activity of Solanine. A Cell Counting Kit‑8 assay demonstrated that Solanine inhibited the proliferation of Jurkat cells in a dose‑and time‑dependent manner. Cell apoptosis was measured by flow cytometry. Flow cytometry revealed that Solanine induced apoptosis in a dose‑dependent manner in Jurkat cells. Reverse transcription‑quantitative polymerase chain reaction demonstrated that Solanine modulated the mRNA levels of B‑cell lymphoma‑2 (Bcl‑2) and Bcl‑2‑associated X protein (Bax). Additionally, Bcl‑2 and Bax expression was measured using western blot analysis. Western blot analysis revealed a significant increase in the expression of Bax and decrease in the expression of Bcl‑2. Solanine increased the chemosensitivity of Jurkat cells to Adriamycin. In summary, the present results indicated that the antitumor activity of Solanine was associated with inhibition of cell proliferation, induction of apoptosis and increasing cytotoxicity of Adriamycin. Therefore, Solanine may have potential as a novel agent for the treatment of acute lymphocytic leukemia.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yi YJ, Jia XH, Wang JY, Chen JR, Wang H and Li YJ: Solanine induced apoptosis and increased chemosensitivity to Adriamycin in T-cell acute lymphoblastic leukemia cells. Oncol Lett 15: 7383-7388, 2018
APA
Yi, Y., Jia, X., Wang, J., Chen, J., Wang, H., & Li, Y. (2018). Solanine induced apoptosis and increased chemosensitivity to Adriamycin in T-cell acute lymphoblastic leukemia cells. Oncology Letters, 15, 7383-7388. https://doi.org/10.3892/ol.2018.8229
MLA
Yi, Y., Jia, X., Wang, J., Chen, J., Wang, H., Li, Y."Solanine induced apoptosis and increased chemosensitivity to Adriamycin in T-cell acute lymphoblastic leukemia cells". Oncology Letters 15.5 (2018): 7383-7388.
Chicago
Yi, Y., Jia, X., Wang, J., Chen, J., Wang, H., Li, Y."Solanine induced apoptosis and increased chemosensitivity to Adriamycin in T-cell acute lymphoblastic leukemia cells". Oncology Letters 15, no. 5 (2018): 7383-7388. https://doi.org/10.3892/ol.2018.8229