Open Access

miR-224 enhances invasion and metastasis by targeting HOXD10 in non-small cell lung cancer cells

  • Authors:
    • Shuang Li
    • Jingang Zhang
    • Yunwei Zhao
    • Fengling Wang
    • Ying Chen
    • Xiubin Fei
  • View Affiliations

  • Published online on: March 12, 2018     https://doi.org/10.3892/ol.2018.8245
  • Pages: 7069-7075
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Increasing number of studies have indicated aberrant microRNA (miRNA) expression could affect normal biological progress in non-small cell lung cancer (NSCLC) cells. This study was performed to evaluate the biologic functions of microRNA-224 (miR-224) in NSCLC. Real-time PCR was performed to evaluate the expression of miR-224 and Homeobox D10 (HOXD10) in NSCLC cell lines and tissues. Transwell assays were performed to investigate the function of miR-224 on NSCLC cell migration and invasion. Moreover, western blotting and luciferase assays were used to investigate HOXD10 as miR-224 downstream targets. miR-224 is increased in NSCLC metastatic tissues and cell lines. Increased miR-224 expression promoted NSCLC cell migration and invasion, while low miR-224 expression suppressed NSCLC cell migration and invasion. Furthermore, HOXD10 was targeted directly by miR-224 in NSCLC cells. Moreover, we found that HOXD10 was a functional target and influenced tumour-inductive functions of miR-224 on progression of NSCLC. These findings suggest that miR-224 may be used in the treatment of NSCLC. Targeting this novel strategy, miR-224/HOXD10 axis may be helpful as promising biomarker and therapeutic method to control NSCLC cell metastasis.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Li S, Zhang J, Zhao Y, Wang F, Chen Y and Fei X: miR-224 enhances invasion and metastasis by targeting HOXD10 in non-small cell lung cancer cells. Oncol Lett 15: 7069-7075, 2018
APA
Li, S., Zhang, J., Zhao, Y., Wang, F., Chen, Y., & Fei, X. (2018). miR-224 enhances invasion and metastasis by targeting HOXD10 in non-small cell lung cancer cells. Oncology Letters, 15, 7069-7075. https://doi.org/10.3892/ol.2018.8245
MLA
Li, S., Zhang, J., Zhao, Y., Wang, F., Chen, Y., Fei, X."miR-224 enhances invasion and metastasis by targeting HOXD10 in non-small cell lung cancer cells". Oncology Letters 15.5 (2018): 7069-7075.
Chicago
Li, S., Zhang, J., Zhao, Y., Wang, F., Chen, Y., Fei, X."miR-224 enhances invasion and metastasis by targeting HOXD10 in non-small cell lung cancer cells". Oncology Letters 15, no. 5 (2018): 7069-7075. https://doi.org/10.3892/ol.2018.8245