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Monoclonal antibody 3H11 chimeric antigen receptors enhance T cell effector function and exhibit efficacy against gastric cancer

  • Authors:
    • Haibo Han
    • Shanshan Wang
    • Ying Hu
    • Zhaowei Li
    • Wei Yang
    • Yunwei Lv
    • Limin Wang
    • Lianhai Zhang
    • Jiafu Ji
  • View Affiliations / Copyright

    Affiliations: Department of Biobank, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital and Institute, Beijing 100142, P.R. China, Department of Clinical Laboratory, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital and Institute, Beijing 100142, P.R. China, Department of Gastrointestinal Surgery, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital and Institute, Beijing 100142, P.R. China
    Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6887-6894
    |
    Published online on: March 13, 2018
       https://doi.org/10.3892/ol.2018.8255
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Abstract

Although chimeric antigen receptor T cell (CAR‑T) therapies for certain types of solid tumors have been used in clinical trials, novel CARs that are able to target gastric cancer (GC) are still required. In our previous study, monoclonal antibody 3H11 (mAb 3H11), generated from immunization with five human GC cell lines, was demonstrated to have a 93.5% positive reaction with a clear membrane location and more than 5% cancer cell staining in GC tissues in our previous study. In the present study, 3H11‑CARs were designed for modified T cell therapy. To begin with, it was confirmed that the single‑chain variable fragment (scFV) of the mAb 3H11, known as scFV‑3H11, exhibited similar activity with the natural antibody. In addition, scFV‑3H11 CAR‑T cells are able to kill tumor cells accompanied with increased interleukin‑2 and interferon‑γ secretion  in vitro, and reduced the tumor burden in GC cell lines and patient‑derived GC cells  in vivo. In conclusion, scFV‑3H11 CARs may have the potential to treat mAb 3H11‑positive GC.
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Copy and paste a formatted citation
Spandidos Publications style
Han H, Wang S, Hu Y, Li Z, Yang W, Lv Y, Wang L, Zhang L and Ji J: Monoclonal antibody 3H11 chimeric antigen receptors enhance T cell effector function and exhibit efficacy against gastric cancer. Oncol Lett 15: 6887-6894, 2018.
APA
Han, H., Wang, S., Hu, Y., Li, Z., Yang, W., Lv, Y. ... Ji, J. (2018). Monoclonal antibody 3H11 chimeric antigen receptors enhance T cell effector function and exhibit efficacy against gastric cancer. Oncology Letters, 15, 6887-6894. https://doi.org/10.3892/ol.2018.8255
MLA
Han, H., Wang, S., Hu, Y., Li, Z., Yang, W., Lv, Y., Wang, L., Zhang, L., Ji, J."Monoclonal antibody 3H11 chimeric antigen receptors enhance T cell effector function and exhibit efficacy against gastric cancer". Oncology Letters 15.5 (2018): 6887-6894.
Chicago
Han, H., Wang, S., Hu, Y., Li, Z., Yang, W., Lv, Y., Wang, L., Zhang, L., Ji, J."Monoclonal antibody 3H11 chimeric antigen receptors enhance T cell effector function and exhibit efficacy against gastric cancer". Oncology Letters 15, no. 5 (2018): 6887-6894. https://doi.org/10.3892/ol.2018.8255
Copy and paste a formatted citation
x
Spandidos Publications style
Han H, Wang S, Hu Y, Li Z, Yang W, Lv Y, Wang L, Zhang L and Ji J: Monoclonal antibody 3H11 chimeric antigen receptors enhance T cell effector function and exhibit efficacy against gastric cancer. Oncol Lett 15: 6887-6894, 2018.
APA
Han, H., Wang, S., Hu, Y., Li, Z., Yang, W., Lv, Y. ... Ji, J. (2018). Monoclonal antibody 3H11 chimeric antigen receptors enhance T cell effector function and exhibit efficacy against gastric cancer. Oncology Letters, 15, 6887-6894. https://doi.org/10.3892/ol.2018.8255
MLA
Han, H., Wang, S., Hu, Y., Li, Z., Yang, W., Lv, Y., Wang, L., Zhang, L., Ji, J."Monoclonal antibody 3H11 chimeric antigen receptors enhance T cell effector function and exhibit efficacy against gastric cancer". Oncology Letters 15.5 (2018): 6887-6894.
Chicago
Han, H., Wang, S., Hu, Y., Li, Z., Yang, W., Lv, Y., Wang, L., Zhang, L., Ji, J."Monoclonal antibody 3H11 chimeric antigen receptors enhance T cell effector function and exhibit efficacy against gastric cancer". Oncology Letters 15, no. 5 (2018): 6887-6894. https://doi.org/10.3892/ol.2018.8255
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