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Na+/H+ exchanger regulatory factor 1 overexpression suppresses the malignant phenotype of MIAPaCa-2 pancreatic adenocarcinoma cells by downregulating Akt phosphorylation

  • Authors:
    • Xibo Zhang
    • Junjian Liu
    • Zhonglian Li
  • View Affiliations / Copyright

    Affiliations: Department of Hepatopancreatobiliary Surgery, Tianjin Nankai Hospital, Tianjin 300100, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7725-7729
    |
    Published online on: March 16, 2018
       https://doi.org/10.3892/ol.2018.8289
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Abstract

Na+/H+ exchanger regulatory factor 1 (NHERF1) is reported to be associated with the development of numerous types of tumor; however, its effects on the metastasis of pancreatic adenocarcinoma are not fully understood. In the present study, it was revealed that the expression level of NHERF1 in pancreatic adenocarcinoma is decreased compared with normal pancreatic tissue based on the analysis of a protein expression database. The present study was undertaken in order to investigate the potential effects of NHERF1 overexpression on the malignant phenotype of MIAPaCa‑2 pancreatic adenocarcinoma cells. NHERF1 was stably overexpressed in this cell line, and Cell Counting Kit‑8, wound healing and Transwell assays were used to detect the proliferative and migratory abilities of the cells. NHERF1 overexpression suppressed proliferation in the MIAPaCa‑2 cell line compared with empty vector‑transfected (negative control) cells. Additionally, NHERF1 overexpression significantly inhibited the migration of MIAPaCa‑2 cells. The results of a western blot analysis identified that NHERF1 overexpression markedly decreased the expression of phosphorylated‑protein kinase B (p‑Akt), while no significant difference was observed between untransfected and negative control cells. Taken together, these results suggested that NHERF1 may be able to inhibit the proliferation and migration and alter the malignant phenotype of pancreatic adenocarcinoma cells via reduction of p‑Akt levels. These findings indicate a potential novel approach to the treatment of pancreatic adenocarcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Liu J and Li Z: Na+/H+ exchanger regulatory factor 1 overexpression suppresses the malignant phenotype of MIAPaCa-2 pancreatic adenocarcinoma cells by downregulating Akt phosphorylation. Oncol Lett 15: 7725-7729, 2018.
APA
Zhang, X., Liu, J., & Li, Z. (2018). Na+/H+ exchanger regulatory factor 1 overexpression suppresses the malignant phenotype of MIAPaCa-2 pancreatic adenocarcinoma cells by downregulating Akt phosphorylation. Oncology Letters, 15, 7725-7729. https://doi.org/10.3892/ol.2018.8289
MLA
Zhang, X., Liu, J., Li, Z."Na+/H+ exchanger regulatory factor 1 overexpression suppresses the malignant phenotype of MIAPaCa-2 pancreatic adenocarcinoma cells by downregulating Akt phosphorylation". Oncology Letters 15.5 (2018): 7725-7729.
Chicago
Zhang, X., Liu, J., Li, Z."Na+/H+ exchanger regulatory factor 1 overexpression suppresses the malignant phenotype of MIAPaCa-2 pancreatic adenocarcinoma cells by downregulating Akt phosphorylation". Oncology Letters 15, no. 5 (2018): 7725-7729. https://doi.org/10.3892/ol.2018.8289
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Liu J and Li Z: Na+/H+ exchanger regulatory factor 1 overexpression suppresses the malignant phenotype of MIAPaCa-2 pancreatic adenocarcinoma cells by downregulating Akt phosphorylation. Oncol Lett 15: 7725-7729, 2018.
APA
Zhang, X., Liu, J., & Li, Z. (2018). Na+/H+ exchanger regulatory factor 1 overexpression suppresses the malignant phenotype of MIAPaCa-2 pancreatic adenocarcinoma cells by downregulating Akt phosphorylation. Oncology Letters, 15, 7725-7729. https://doi.org/10.3892/ol.2018.8289
MLA
Zhang, X., Liu, J., Li, Z."Na+/H+ exchanger regulatory factor 1 overexpression suppresses the malignant phenotype of MIAPaCa-2 pancreatic adenocarcinoma cells by downregulating Akt phosphorylation". Oncology Letters 15.5 (2018): 7725-7729.
Chicago
Zhang, X., Liu, J., Li, Z."Na+/H+ exchanger regulatory factor 1 overexpression suppresses the malignant phenotype of MIAPaCa-2 pancreatic adenocarcinoma cells by downregulating Akt phosphorylation". Oncology Letters 15, no. 5 (2018): 7725-7729. https://doi.org/10.3892/ol.2018.8289
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