Open Access

Evaluating the prognostic value and functional roles of transcription factor AP4 in colorectal cancer

  • Authors:
    • Jie Yang
    • Jin‑Ping Ma
    • Siyu Xiao
    • Xin‑Hua Zhang
    • Jian‑Bo Xu
    • Chuang‑Qi Chen
    • Shi‑Rong Cai
    • Yu‑Long He
  • View Affiliations

  • Published online on: March 16, 2018     https://doi.org/10.3892/ol.2018.8290
  • Pages: 7545-7554
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The basic helix-loop-helix transcription factor AP4 (TFAP4) gene serves an important function in the genesis and progression of tumors. However, few studies to date have defined the role of this gene in colorectal cancer (CRC). The aim of the present study was to assess the expression of TFAP4 in CRC and its impact on the prognosis of patients with CRC. In the present study, the expression of TFAP4 was detected in 30 matched pairs of fresh CRC tissues, 187 cases of clinical paraffin‑embedded CRC tissues and CRC cell lines using the reverse transcriptase‑quantitative polymerase chain reaction, immunohistochemistry or western blot analysis. Survival analysis was based on TFAP4 expression. The effects of TFAP4 on CRC cell function were investigated by ectopic expression or knockdown of TFAP4 in vitro. TFAP4 expression was revealed to be increased in human CRC tissues and cell lines. The overall survival (OS) time of patients with high TFAP4 expression was significantly decreased compared with patients with low TFAP4 expression (P<0.001). In addition, TFAP4 was revealed to be an independent prognostic factor for the OS time of patients with CRC (hazard ratio, 2.607; 95% confidence interval, 1.469‑4.627; P=0.001). Ectopic TFAP4 expression promoted CRC cell proliferation, migration and invasion in vitro, and the silencing of TFAP4 expression resulted in the inhibition of these events. These results demonstrated that TFAP4, which was overexpressed in CRC tissues and cell lines, increased the malignant potential of CRC cells and may serve as an indicator for poor prognosis in patients with CRC.
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May-2018
Volume 15 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Yang J, Ma JP, Xiao S, Zhang XH, Xu JB, Chen CQ, Cai SR and He YL: Evaluating the prognostic value and functional roles of transcription factor AP4 in colorectal cancer. Oncol Lett 15: 7545-7554, 2018.
APA
Yang, J., Ma, J., Xiao, S., Zhang, X., Xu, J., Chen, C. ... He, Y. (2018). Evaluating the prognostic value and functional roles of transcription factor AP4 in colorectal cancer. Oncology Letters, 15, 7545-7554. https://doi.org/10.3892/ol.2018.8290
MLA
Yang, J., Ma, J., Xiao, S., Zhang, X., Xu, J., Chen, C., Cai, S., He, Y."Evaluating the prognostic value and functional roles of transcription factor AP4 in colorectal cancer". Oncology Letters 15.5 (2018): 7545-7554.
Chicago
Yang, J., Ma, J., Xiao, S., Zhang, X., Xu, J., Chen, C., Cai, S., He, Y."Evaluating the prognostic value and functional roles of transcription factor AP4 in colorectal cancer". Oncology Letters 15, no. 5 (2018): 7545-7554. https://doi.org/10.3892/ol.2018.8290