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Article

Effects of sulindac sulfide on proliferation and apoptosis of human breast cancer cell

  • Authors:
    • He‑Huan Sui
    • Yun‑Jiang Zhou
    • Hu Wang
    • Li Li
    • Min Cao
    • Jia‑Jun Huang
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Chongqing Medical University, Chongqing 400016, P.R. China
  • Pages: 7981-7986
    |
    Published online on: March 23, 2018
       https://doi.org/10.3892/ol.2018.8331
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Abstract

The present study aimed to observe the effects of sulindac sulfide on the proliferation and apoptosis of human breast cancer cells MCF‑7, and to explore the potential underlying molecular mechanism. The inhibitory ratio was detected using a cell counting kit‑8 assay. The changes in cell cycle distribution were assessed using flow cytometry (FCM). Furthermore, the changes in cell apoptosis rates were detected by Hoechst 33258 staining and FCM coupled with Annexin V‑FITC/propidium iodide (PI) staining. In addition, the protein expression was detected using western blotting. Sulindac sulfide was able to inhibit the proliferation of breast cancer in a dose‑ and time‑dependent manner. In addition, sulindac sulfide altered the cell cycle of breast cancer cells. The results of Hoechst 33258 staining and FCM coupled with Annexin V‑FITC/PI staining demonstrated that sulindac sulfide could significantly induce the apoptosis of MCF‑7 cells in a dose‑dependent, and time‑dependent manner. The western blot analysis demonstrated the protein expression of Bcl‑2 was downregulated, and Bax and cleaved caspase‑3 were upregulated. The results of the present study suggest that sulindac sulfide can inhibit the proliferation and induce the apoptosis of MCF‑7 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Sui HH, Zhou YJ, Wang H, Li L, Cao M and Huang JJ: Effects of sulindac sulfide on proliferation and apoptosis of human breast cancer cell. Oncol Lett 15: 7981-7986, 2018.
APA
Sui, H., Zhou, Y., Wang, H., Li, L., Cao, M., & Huang, J. (2018). Effects of sulindac sulfide on proliferation and apoptosis of human breast cancer cell. Oncology Letters, 15, 7981-7986. https://doi.org/10.3892/ol.2018.8331
MLA
Sui, H., Zhou, Y., Wang, H., Li, L., Cao, M., Huang, J."Effects of sulindac sulfide on proliferation and apoptosis of human breast cancer cell". Oncology Letters 15.5 (2018): 7981-7986.
Chicago
Sui, H., Zhou, Y., Wang, H., Li, L., Cao, M., Huang, J."Effects of sulindac sulfide on proliferation and apoptosis of human breast cancer cell". Oncology Letters 15, no. 5 (2018): 7981-7986. https://doi.org/10.3892/ol.2018.8331
Copy and paste a formatted citation
x
Spandidos Publications style
Sui HH, Zhou YJ, Wang H, Li L, Cao M and Huang JJ: Effects of sulindac sulfide on proliferation and apoptosis of human breast cancer cell. Oncol Lett 15: 7981-7986, 2018.
APA
Sui, H., Zhou, Y., Wang, H., Li, L., Cao, M., & Huang, J. (2018). Effects of sulindac sulfide on proliferation and apoptosis of human breast cancer cell. Oncology Letters, 15, 7981-7986. https://doi.org/10.3892/ol.2018.8331
MLA
Sui, H., Zhou, Y., Wang, H., Li, L., Cao, M., Huang, J."Effects of sulindac sulfide on proliferation and apoptosis of human breast cancer cell". Oncology Letters 15.5 (2018): 7981-7986.
Chicago
Sui, H., Zhou, Y., Wang, H., Li, L., Cao, M., Huang, J."Effects of sulindac sulfide on proliferation and apoptosis of human breast cancer cell". Oncology Letters 15, no. 5 (2018): 7981-7986. https://doi.org/10.3892/ol.2018.8331
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