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Acute pro‑B‑Cell lymphoblastic leukemia transformed from myelodysplastic syndrome with an ASXL1 missense mutation: A case report with literature review

  • Authors:
    • Zhi‑Ping Guo
    • Yan‑Hong Tan
    • Jian‑Lan Li
    • Zhi‑Fang Xu
    • Xiu‑Hua Chen
    • Lian‑Rong Xu
  • View Affiliations

  • Published online on: April 20, 2018     https://doi.org/10.3892/ol.2018.8546
  • Pages: 9745-9750
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The development of acute lymphoblastic leukemia (ALL) from myelodysplastic syndrome (MDS) is a very rare event. The current report presents a rare case of a 33‑year‑old man who was diagnosed with MDS with multiple‑lineage dysplasia (MDS‑MLD) that transformed into pro‑B‑ALL. A missense mutation (S1231F) of the additional sex combs like 1, transcriptional regulator gene was identified, which may have a substantial role in the progression, however does not act as an unfavorable prognostic marker. The patient died during induction chemotherapy. The present study further conducted an analysis on 30 patients to determine progression to ALL. Patients were predominantly male (76.7%, 23/30) with a median age of 56 years (3‑90 years). The median time to transformation was 5.5 months (2‑50 months). The most common type of MDS with ALL transformation comprised of MDS‑excess blasts (MDS‑EB; 40%, 12/30), MDS with single‑lineage dysplasia (MDS‑SLD; 30%, 9/30) and MDS with ring sideroblasts (MDS‑RS; 16.7%, 5/30). The majority of the patients transformed to B‑cell (66.7%, 16/24) followed by T‑cell (33.3%, 8/24) ALL. From the 25 cases where data was available, the complete remission rate was 75% (15/20) with ALL‑directed chemotherapy and the median remission duration was 15 months (range 4.5 to 51 months). However, the results indicated that ALL following MDS is characterized by a high rate of early death (20%, 5/25).

Introduction

Myelodysplastic syndromes (MDSs) are a heterogeneous clonal disease originating in hematopoietic stem cells and characterized by myeloid dysplasia, impaired differentiation, and peripheral cytopenia (1), with estimated incidence rate of 4.3 per 100,000 (2). MDS is recognized as a preleukaemic event in which neoplastic clones are established. It frequently evolves to acute leukemia in 20–40% of MDS patients (3). MDS originates from multipotent hematopoietic stem cells, which can give rise to both lymphoid and myeloid leukemic cells (4). However, progression of MDS to acute lymphocytic leukemia (ALL) is extremely rare, occurring in less than 1% of cases (3,5,6). To date, there are only 29 reported cases of MDS progressing to ALL. The pathogenesis and prognosis of ALL transformed from MDS is still unclear.

With the advancement of next-generation sequencing, it is possible to identify new somatic mutations that have a potential impact on MDS patients. Mutations in additional sex combs like-1 (ASXL1), an unfavorable prognostic marker, occurs with a frequency of 10–20% in MDS, with 70% being frameshift mutations and 30% being heterozygous point mutations (79). Frameshift or nonsense mutations correlated with shorter survival rates and a higher risk of leukemic transformation (8,10,11). However, the clinical significance of ASXL1 missense mutations is still questionable. Further research to the role of ASXL1 missense mutations in MDS is required.

In this report, we describe a Chinese male patient harboring an ASXL1 missense mutation. He was diagnosed with pro-B-ALL, transformed from MDS with multiple-lineage dysplasia (MDS-MLD). Furthermore, in order to gain full insight into the clinical features and treatment outcome of ALL transformed from MDS, we analyzed previously reported data from 30 patients for such progression.

Case report

A 33-year-old Chinese man was evaluated for thrombocytopenia in July 2013. On examination, he had splenomegaly. Initial blood counts revealed the following; hemoglobin, 123 g/l; leukocyte count, 7.23×109/l; and platelet count, 23×109/l. Bone marrow examination (Fig. 1) revealed augmented cellularity and trilineage dysplasia with no increase in myeloblasts dysplasia. Cytogenic testing revealed a normal karyotype 46, XY. Using specific and comprehensive fluorescent in situ hybridization (FISH) probe panels showed a lack of demonstrable cytogenetic abnormalities. The patient was diagnosed with MDS-MLD using the World Health Organization classification. Using the revised International Prognostic Scoring System (IPSS-R), he was classified into the low risk category. The patient exhibited persistent thrombocytopenia with progressing anemia. He did not receive any treatment.

After a 47-month history of prolonged bleeding and fatigue, his condition worsened abruptly. Blood counts demonstrated hemoglobin levels at 49 g/l; platelets at 5×109/l; and leukocyte counts at 6.56×109/l. Examination of bone marrow was consistent with a diagnosis of ALL, revealing 63.5% blasts (Fig. 2A) with numerous micromegakaryocytes by CD41 monoclonal antibody immune enzyme staining (Fig. 2B). Cytochemical staining demonstrated that the blasts were 95% negative for peroxidase (Fig. 2C). Cell surface markers analyzed by flow cytometry were positive for CD34, CD19, cCD79a, CD123, and CD38. The blasts also expressed CD33, a myeloid marker. Myeloid cell markers for CD117 and CD13 and T cell markers for cyCD3, CD5, and CD7 were not expressed. The blasts were also negative for CD10, CD15, CD11b, CD64, CD14, CD20, cTDT, cIgM, CD56 and cyMPO. Based on these markers, the patient was diagnosed with Pro-B-ALL. Karyotype analysis of bone marrow leukemic cells revealed normal chromosomes (46, XY). Gene rearrangements were not detected using reverse transcription-PCR.

In addition, known gene mutations associated with MDS were screened using next-generation sequencing techniques. Driver mutant genes include those of RNA splicing (SF3B1, SRSF2 and U2AF1), DNA methylation (TET2, DNMT3A, and IDH1/2), chromatin modification (ASXL1 and EZH2), transcription regulation (RUNX1), DNA repair (TP53), and signal transduction (NRAS and KRAS). The patient harbored the point mutation TCC>TTC in codon 1231 of ASXL1 gene (Fig. 3), leading to a serine to phenylalanine substitution (S1231F). Other genetic aberrations were not detected in this patient. Subsequently, the patient received chemotherapy beginning the day after bone marrow examination. Treatment regimen included prednisone, vincristine, cyclophosphamide and idarubicin. Unfortunately, the patient passed away due to severe pulmonary infection during the course of chemotherapy. Written informed consent for publication of data was obtained from patient's family.

Discussion

We performed an analysis of 30 patients with MDS transforming to ALL (summary in Table I). Clinical characteristics were similar with previous reports in literature (12,13). Patients were predominantly male (76.7%, 23/30) with a median age of 56 years (3–90 years). The median time to transformation was 5.5 months (2–50 months). The common types of MDS with ALL transformation include MDS-excess blasts (MDS-EB) (40%, 12/30), MDS with single-lineage dysplasia (MDS-SLD) (30%, 9/30) and MDS with ring sideroblasts (MDS-RS) (16.7%, 5/30). Majority of the patients transformed to B-cell (66.7%, 16/24) followed by T-cell (33.3%, 8/24) ALL. Using immunophenotyping analysis of our 33 year old male patient, we confirmed the diagnosis of pro-B-ALL due to the bright uniform expression of CD19 and the absence of CD10 surface antigen. The coexpression of CD34 and CD33 myeloid antigens was consistent with the immunophenotypic subtype of pro-B-ALL. Our patient had absence of MLL1/AF4 and BCR/ABL fusion genes, which correlates with a significantly better clinical outcome (14). Because of this, our patient was expected to have a good prognosis. However, after a period of prolonged fatigue and bleeding, his condition rapidly deteriorated and he eventually died of severe pulmonary infection due to excess myelosuppression during ALL-directed chemotherapy. His death may be attributed to the nature of MDS transformation.

Table I.

Previously reported cases of lymphoid transformation of myelodysplastic syndrome.

Table I.

Previously reported cases of lymphoid transformation of myelodysplastic syndrome.

No.Age (y)SexTime to transformation (months)Initial diagnosis of MDSPhenotype of blast at transformationChromosomal abnormalitiesOutcomeSurvival(Refs.)
  168M31RSB-cell+8Early death(6)
  268F5EBB-cell45XX, −3, del(5)(q13q31)[4]/46,XX[16]Death during induction(17)
  343M11Eosinophilic MDSB-cell45,XY, del(5q)(q22q23), −7, add(12)(p13)[5]Death during induction(18)
  458M20RALnot availableDeath during induction(19)
  533M47MLDB-ALLNormalDeath during inductionCurrent case
  667M18EBT-cell45XY, −7Fail(15)
  778M5RST-cellFail(15)
  854M30SLDB-cell 46,XY,t(9;22)(q34;q11), 20q-Refractory(20)
  928F11SLDB-cellNormalRefractory(21)
1065M18SLDB-cell+13, partially tetraploidPR (22)
1153F2MDS with myelofibrosisB cellNormalCRAlive(12)
12  3M EBPre-B-cell CR(23)
13  4MNAEBALL5q-CR19 months(24)
14  5M4EBB cellnot availableCRAlive(13)
1546M2EBPre B cellCRAlive(25)
1650M4SLDB-cellComplexCR(26)
1767M24RSAneuploidyCR12 months(27)
1853M50RSNull cellnot availableCR11 months(28)
1920M5EBT cellnot availableCR24 months(29)
2050F3EBcALLNormalCRAlive(30)
2165M4EBT-cell46XY[7]/47XY, +8[7]/48XY, +8,+13[1]/48XY, +8,+20[1]CR51 months(15)
2272F5RST-cellNormalCR4.5 months(15)
2369M6SLDT-cell46XY[9]/47XY, +8[1]CR15 months(15)
2472M5EBT-cellNormalCR12 months(15)
2562F2SLDB-cellCR25 months(31)
26  9M21SLDB-cellnot availablenot available(32)
2790M5EBcALLnot donenot available(33)
2875M18EBT-cellNormalnot available(34)
2970M22SLDB-cellnot available(35)
30  8F3RCCB cellNormalnot available(36)

Although AML evolved from MDS has a poor prognosis, ALL following MDS has not been associated with a poor prognosis as reported in the literature (15). However, due to the limited number of cases (9 cases), it is difficult to make any conclusions regarding prognosis. From the 25 cases of MDS transforming to ALL reported in the literature (Table I), 15 patients achieved complete remission (CR) and 1 achieved partial remission (PR). Two cases were refractory and 2 patients failed to achieve a CR after induction treatment. Four patients died during induction therapy and 1 died before therapy. Our results confirm that the complete remission (CR) rate (75%, 15/20) with ALL-directed chemotherapy and the median remission duration of 15 months (range 4.5 to 51 months) are similar with previous reports (15). However, ALL following MDS is characterized by a higher rate of early mortality (20%, 5/25).

Genetic aberrations appear to play an important role in the pathogenesis of MDS. ASXL1 gene mutations are one of the characteristic mutations observed for MDS. It encodes a highly conserved protein that belongs to the enhancer of trithorax and polycomb (ETP) genes, a gene family with dual functions in both epigenetic activation and repression of gene transcription (16). It contains several nuclear receptor binding motifs and a carboxy-terminal plant homeodomain (PHD) that is predicted to be truncated by frameshift mutations or nonsense mutations (10,11). However, the clinical significance of missense mutations in ASXL1 is unknown. An ASXL1 missense mutation was detected in our patient. This ASXL1 missense mutation did not correspond with other gene mutations identified as adverse prognostic factors. We hypothesize that it may play a substantial role in the progression of our case. The function of ASXL1 is expected to be impaired by this missense mutation.

These novel genetic aberrations in MDS need to be further evaluated regarding their prognostic impact. In our patient, the time to transformation after primary diagnosis was much longer than the median observed. It seems that this missense mutation, unlike frameshift mutations of ASXL1, was not an unfavorable prognostic marker for patients with MDS, which is consistent with previous reports (9). Additional studies needs to be conducted to elucidate the influence of ASXL1 missense mutations on the prognosis of patients with MDS.

In summary, we report an ASXL1 missense mutation potentially leading to MDS-MLD transforming to pro-B ALL. This mutation might provide insight into the pathogenesis and prognosis of MDS. ALL following MDS responds well to ALL-directed therapy, but higher rates of early mortality are observed. The prognosis of ALL following MDS needs to be further validated using more uniformly treated patients within a given protocol. Identification of specific gene mutations will hopefully provide for better treatment strategies for this group of patients.

Acknowledgements

The authors would like to thanks the faculty members who analyzed the cytogenetic data.

Funding

Nor applicable.

Availability of data and materials

All data generated or analyzed during this study are included in this published article.

Authors' contributions

LX and ZG analyzed and interpreted the patient data regarding the hematological disease. ZG was a major contributor in designing the work and writing the manuscript. YT and XC performed the PCR and next-generation sequencing. ZX analyzed the flow cytometric data. JL performed the bone marrow examination. All authors read and approved the final manuscript.

Ethics approval and consent to participate

The study was granted an exemption from requiring ethics approval from the ethics committee of the Second Hospital of Shanxi Medical University.

Consent for publication

Informed consent for publication was obtained from the family of the patient reported in current study.

Competing interests

The authors declare that they have no competing interests.

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Spandidos Publications style
Guo ZP, Tan YH, Li JL, Xu ZF, Chen XH and Xu LR: Acute pro‑B‑Cell lymphoblastic leukemia transformed from myelodysplastic syndrome with an ASXL1 missense mutation: A case report with literature review. Oncol Lett 15: 9745-9750, 2018.
APA
Guo, Z., Tan, Y., Li, J., Xu, Z., Chen, X., & Xu, L. (2018). Acute pro‑B‑Cell lymphoblastic leukemia transformed from myelodysplastic syndrome with an ASXL1 missense mutation: A case report with literature review. Oncology Letters, 15, 9745-9750. https://doi.org/10.3892/ol.2018.8546
MLA
Guo, Z., Tan, Y., Li, J., Xu, Z., Chen, X., Xu, L."Acute pro‑B‑Cell lymphoblastic leukemia transformed from myelodysplastic syndrome with an ASXL1 missense mutation: A case report with literature review". Oncology Letters 15.6 (2018): 9745-9750.
Chicago
Guo, Z., Tan, Y., Li, J., Xu, Z., Chen, X., Xu, L."Acute pro‑B‑Cell lymphoblastic leukemia transformed from myelodysplastic syndrome with an ASXL1 missense mutation: A case report with literature review". Oncology Letters 15, no. 6 (2018): 9745-9750. https://doi.org/10.3892/ol.2018.8546