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Knockdown of p57 gene inhibits breast cancer cell proliferation

  • Authors:
    • Tai Ping Zhao
    • Xin Liang Wang
    • Yi Min Han
  • View Affiliations / Copyright

    Affiliations: College of Health Sciences, Guangzhou Medical University, Guangzhou, Guangdong 510450, P.R. China, Guangzhou First People's Hospital, Guangzhou, Guangdong 510180, P.R. China, Department of Oncology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150081, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 55-58
    |
    Published online on: May 2, 2018
       https://doi.org/10.3892/ol.2018.8605
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Abstract

The aim of the study was to investigate possible effects of p57 on the growth of the human MCF‑7 and rat SHZ‑88 breast cancer cell lines. Specific oligonucleotide sequences containing small hairpin structure were inserted into a small interfering RNA (siRNA) expression vector. The human MCF‑7 and rat SHZ‑88 breast cancer cell lines were transfected with recombinant plasmids. The p57 gene expression was blocked in the human MCF‑7 breast and rat SHZ‑88 breast cancer cells, using chemically modified siRNA. The p57 expression level was evaluated using quantitative polymerase chain reaction (qPCR) and western blot analysis. Immunofluorescence was conducted to detect p57 expression in the breast cancer cells. Tetrazolium blue (MTT) method was employed to detect the effect of p57 inhibition on the proliferation of the MCF‑7 and SHZ‑88 cell lines. Cell proliferation in the experimental group was significantly reduced. Immunofluorescence assay results showed p57 siRNA effectively inhibited the p57 level in the MCF‑7 and SHZ‑88 cells. RT‑PCR results showed that 48 h after transfection, the p57 mRNA level in the transfected group was significantly lower compared with the control group. In conclusion, p57 effectively inhibited the proliferation of breast cancer after stable interference.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao TP, Wang XL and Han YM: Knockdown of p57 gene inhibits breast cancer cell proliferation. Oncol Lett 16: 55-58, 2018.
APA
Zhao, T.P., Wang, X.L., & Han, Y.M. (2018). Knockdown of p57 gene inhibits breast cancer cell proliferation. Oncology Letters, 16, 55-58. https://doi.org/10.3892/ol.2018.8605
MLA
Zhao, T. P., Wang, X. L., Han, Y. M."Knockdown of p57 gene inhibits breast cancer cell proliferation". Oncology Letters 16.1 (2018): 55-58.
Chicago
Zhao, T. P., Wang, X. L., Han, Y. M."Knockdown of p57 gene inhibits breast cancer cell proliferation". Oncology Letters 16, no. 1 (2018): 55-58. https://doi.org/10.3892/ol.2018.8605
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao TP, Wang XL and Han YM: Knockdown of p57 gene inhibits breast cancer cell proliferation. Oncol Lett 16: 55-58, 2018.
APA
Zhao, T.P., Wang, X.L., & Han, Y.M. (2018). Knockdown of p57 gene inhibits breast cancer cell proliferation. Oncology Letters, 16, 55-58. https://doi.org/10.3892/ol.2018.8605
MLA
Zhao, T. P., Wang, X. L., Han, Y. M."Knockdown of p57 gene inhibits breast cancer cell proliferation". Oncology Letters 16.1 (2018): 55-58.
Chicago
Zhao, T. P., Wang, X. L., Han, Y. M."Knockdown of p57 gene inhibits breast cancer cell proliferation". Oncology Letters 16, no. 1 (2018): 55-58. https://doi.org/10.3892/ol.2018.8605
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