Open Access

Knockdown of p57 gene inhibits breast cancer cell proliferation

  • Authors:
    • Tai Ping Zhao
    • Xin Liang Wang
    • Yi Min Han
  • View Affiliations

  • Published online on: May 2, 2018     https://doi.org/10.3892/ol.2018.8605
  • Pages: 55-58
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the study was to investigate possible effects of p57 on the growth of the human MCF‑7 and rat SHZ‑88 breast cancer cell lines. Specific oligonucleotide sequences containing small hairpin structure were inserted into a small interfering RNA (siRNA) expression vector. The human MCF‑7 and rat SHZ‑88 breast cancer cell lines were transfected with recombinant plasmids. The p57 gene expression was blocked in the human MCF‑7 breast and rat SHZ‑88 breast cancer cells, using chemically modified siRNA. The p57 expression level was evaluated using quantitative polymerase chain reaction (qPCR) and western blot analysis. Immunofluorescence was conducted to detect p57 expression in the breast cancer cells. Tetrazolium blue (MTT) method was employed to detect the effect of p57 inhibition on the proliferation of the MCF‑7 and SHZ‑88 cell lines. Cell proliferation in the experimental group was significantly reduced. Immunofluorescence assay results showed p57 siRNA effectively inhibited the p57 level in the MCF‑7 and SHZ‑88 cells. RT‑PCR results showed that 48 h after transfection, the p57 mRNA level in the transfected group was significantly lower compared with the control group. In conclusion, p57 effectively inhibited the proliferation of breast cancer after stable interference.
View Figures
View References

Related Articles

Journal Cover

July-2018
Volume 16 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhao TP, Wang XL and Han YM: Knockdown of p57 gene inhibits breast cancer cell proliferation. Oncol Lett 16: 55-58, 2018.
APA
Zhao, T.P., Wang, X.L., & Han, Y.M. (2018). Knockdown of p57 gene inhibits breast cancer cell proliferation. Oncology Letters, 16, 55-58. https://doi.org/10.3892/ol.2018.8605
MLA
Zhao, T. P., Wang, X. L., Han, Y. M."Knockdown of p57 gene inhibits breast cancer cell proliferation". Oncology Letters 16.1 (2018): 55-58.
Chicago
Zhao, T. P., Wang, X. L., Han, Y. M."Knockdown of p57 gene inhibits breast cancer cell proliferation". Oncology Letters 16, no. 1 (2018): 55-58. https://doi.org/10.3892/ol.2018.8605