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Article

Downregulation of Glutathione S‑transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line

  • Authors:
    • Huan Liu
    • Zhouping Yang
    • Linquan Zang
    • Guixiang Wang
    • Sigui Zhou
    • Guifang Jin
    • Zhicheng Yang
    • Xuediao Pan
  • View Affiliations / Copyright

    Affiliations: College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China, Department of Pharmacy, Guangdong 999 Brain Hospital, Guangzhou, Guangdong 510510, P.R. China
  • Pages: 467-474
    |
    Published online on: May 2, 2018
       https://doi.org/10.3892/ol.2018.8608
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Abstract

Glutathione S‑transferase A1 (GSTA1) is a phase II detoxification enzyme and serves a crucial role in anti‑cancer drug resistance. In our previous study, GSTA1 was identified to be highly expressed in various subtypes of non‑small‑cell lung cancer cell lines compared with human embryonic lung fibroblast cell line MRC‑5. The aim of the present study was to investigate the effect of GSTA1 expression on the proliferation and apoptosis of A549 cells. GSTA1 expression was knocked down or with overexpressed using lentivirus particles. Western blot analysis and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) were used to assess the protein, and mRNA levels of GSTA1 in A549 cells, respectively. The effect of GSTA1 manipulation on cell proliferation and apoptosis were investigated in vitro using MTT assays, Hoechst 33258 staining and flow cytometry, and in vivo using A549 cell line xenografts in nude mice. The results of the western blot analysis and RT‑qPCR revealed that stable cell models of GSTA1 knockdown, and overexpression were established. The data of the MTT assay indicated that the downregulation of GSTA1 significantly inhibited cell proliferation compared with si‑control‑transfected cells. These si‑GSTA1 A549 cells exhibited typical morphological changes of apoptosis, including chromatin condensation and shrunken nuclei compared with the si‑control counterparts. An AnnexinV‑fluorescein isothiocyanate assay verified that the downregulation of GSTA1 significantly induced cell apoptosis in vitro. In addition, overexpression of GSTA1 significantly promoted tumor growth in vivo. Accordingly, downregulation of GSTA1 suppressed tumor growth. In conclusion, GSTA1 plays an important role in regulation of cell proliferation and cell apoptosis in A549 cell line.
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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Yang Z, Zang L, Wang G, Zhou S, Jin G, Yang Z and Pan X: Downregulation of Glutathione S‑transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line. Oncol Lett 16: 467-474, 2018.
APA
Liu, H., Yang, Z., Zang, L., Wang, G., Zhou, S., Jin, G. ... Pan, X. (2018). Downregulation of Glutathione S‑transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line. Oncology Letters, 16, 467-474. https://doi.org/10.3892/ol.2018.8608
MLA
Liu, H., Yang, Z., Zang, L., Wang, G., Zhou, S., Jin, G., Yang, Z., Pan, X."Downregulation of Glutathione S‑transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line". Oncology Letters 16.1 (2018): 467-474.
Chicago
Liu, H., Yang, Z., Zang, L., Wang, G., Zhou, S., Jin, G., Yang, Z., Pan, X."Downregulation of Glutathione S‑transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line". Oncology Letters 16, no. 1 (2018): 467-474. https://doi.org/10.3892/ol.2018.8608
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Yang Z, Zang L, Wang G, Zhou S, Jin G, Yang Z and Pan X: Downregulation of Glutathione S‑transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line. Oncol Lett 16: 467-474, 2018.
APA
Liu, H., Yang, Z., Zang, L., Wang, G., Zhou, S., Jin, G. ... Pan, X. (2018). Downregulation of Glutathione S‑transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line. Oncology Letters, 16, 467-474. https://doi.org/10.3892/ol.2018.8608
MLA
Liu, H., Yang, Z., Zang, L., Wang, G., Zhou, S., Jin, G., Yang, Z., Pan, X."Downregulation of Glutathione S‑transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line". Oncology Letters 16.1 (2018): 467-474.
Chicago
Liu, H., Yang, Z., Zang, L., Wang, G., Zhou, S., Jin, G., Yang, Z., Pan, X."Downregulation of Glutathione S‑transferase A1 suppressed tumor growth and induced cell apoptosis in A549 cell line". Oncology Letters 16, no. 1 (2018): 467-474. https://doi.org/10.3892/ol.2018.8608
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