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Article

Fentanyl inhibits proliferation and invasion via enhancing miR‑302b expression in esophageal squamous cell carcinoma

  • Authors:
    • Ning Wang
    • Zhenni Zhang
    • Jianrui Lv
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Second Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi'an, Shaanxi 710002, P.R. China
  • Pages: 459-466
    |
    Published online on: May 2, 2018
       https://doi.org/10.3892/ol.2018.8616
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Abstract

Fentanyl is one of the most commonly used intravenous anesthetic agents during cancer resection surgery, but the effect of fentanyl on esophageal squamous cell carcinoma (ESCC) remains unclear. The aim of the present study was to investigate the involvement of microRNA 302b (miR‑302b) in the anti‑proliferation and anti‑invasion effects of fentanyl in ESCC. In the present study, the effects of fentanyl on cell proliferation, apoptosis and invasion were detected using MTT assays, flow cytometry and Transwell assays in ESCC Eca109 and TE1 cell lines. Subsequently, expression of miR‑302b was determined using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). RT‑qPCR and western blot analysis were performed in order to evaluate the expression of ErbB4, a target of miR‑302b. Furthermore, anti‑miR were used to inhibit miR‑302b in fentanyl‑treated ESCC cells in order to evaluate the role of miR‑302b in the effect of fentanyl on malignant behaviors. Fentanyl inhibited the proliferation of Eca109 and TE1 cells in a dose‑ and time‑dependent manner. Following exposure to fentanyl for 48 h, Eca109 and TE1 cells exhibited increased apoptosis and decreased invasion. Furthermore, fentanyl upregulated miR‑302b expression, but downregulated ErbB4 expression. Finally, loss of miR‑302b using the anti‑miR technique reversed the effect of fentanyl on cell proliferation, apoptosis and invasion in the two ESCC cell lines. Taken together, the results of the present study indicated that fentanyl inhibits the proliferation and invasion of ESCC cells through upregulation of miR‑302b.
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Copy and paste a formatted citation
Spandidos Publications style
Wang N, Zhang Z and Lv J: Fentanyl inhibits proliferation and invasion via enhancing miR‑302b expression in esophageal squamous cell carcinoma. Oncol Lett 16: 459-466, 2018.
APA
Wang, N., Zhang, Z., & Lv, J. (2018). Fentanyl inhibits proliferation and invasion via enhancing miR‑302b expression in esophageal squamous cell carcinoma. Oncology Letters, 16, 459-466. https://doi.org/10.3892/ol.2018.8616
MLA
Wang, N., Zhang, Z., Lv, J."Fentanyl inhibits proliferation and invasion via enhancing miR‑302b expression in esophageal squamous cell carcinoma". Oncology Letters 16.1 (2018): 459-466.
Chicago
Wang, N., Zhang, Z., Lv, J."Fentanyl inhibits proliferation and invasion via enhancing miR‑302b expression in esophageal squamous cell carcinoma". Oncology Letters 16, no. 1 (2018): 459-466. https://doi.org/10.3892/ol.2018.8616
Copy and paste a formatted citation
x
Spandidos Publications style
Wang N, Zhang Z and Lv J: Fentanyl inhibits proliferation and invasion via enhancing miR‑302b expression in esophageal squamous cell carcinoma. Oncol Lett 16: 459-466, 2018.
APA
Wang, N., Zhang, Z., & Lv, J. (2018). Fentanyl inhibits proliferation and invasion via enhancing miR‑302b expression in esophageal squamous cell carcinoma. Oncology Letters, 16, 459-466. https://doi.org/10.3892/ol.2018.8616
MLA
Wang, N., Zhang, Z., Lv, J."Fentanyl inhibits proliferation and invasion via enhancing miR‑302b expression in esophageal squamous cell carcinoma". Oncology Letters 16.1 (2018): 459-466.
Chicago
Wang, N., Zhang, Z., Lv, J."Fentanyl inhibits proliferation and invasion via enhancing miR‑302b expression in esophageal squamous cell carcinoma". Oncology Letters 16, no. 1 (2018): 459-466. https://doi.org/10.3892/ol.2018.8616
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