Talin2 regulates breast cancer cell migration and invasion by apoptosis

Liang,Yingfan; Chen,Hongwei; Ji,Ling; Du,Jinfu; Xie,Xiaofan; Li,Xiang; Lou,Yongliang

doi:10.3892/ol.2018.8641

Talin2 regulates breast cancer cell migration and invasion by apoptosis

Authors:
- Yingfan Liang
- Hongwei Chen
- Ling Ji
- Jinfu Du
- Xiaofan Xie
- Xiang Li
- Yongliang Lou
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Affiliations: Zhejiang Provincial Key Laboratory of Medical Genetics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, Suzhou University of Science and Technology, Suzhou, Jiangsu 215009, P.R. China, Department of Colorectal Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, Renji College, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China
Published online on: May 4, 2018 https://doi.org/10.3892/ol.2018.8641
Pages: 285-293
Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Talin is a key component molecule of the extracellular matrix‑integrin‑cytoskeleton. It serves an important role in the activation of integrin, which, in turn, is known to mediate physiological and pathological processes, including cell adhesion, growth, tumorigenesis, and metastasis. In vertebrates, there are two Talin genes, Talin1 and Talin2. Talin1 is known to regulate focal adhesion dynamics, cell migration and cell invasion; however, the precise role of Talin2 in cancer remains unclear. In the present study, the functional role of Talin2 was examined in the MDA‑MB‑231 breast cancer cell line. Talin2 knockdown significantly inhibited growth, migratory capacity and invasiveness of MDA‑MB‑231 cells, and promoted apoptosis. The expression levels of Talin2 in breast cancer cells and in the peritumoral normal breast tissues were also determined by immunohistochemistry. Talin2 was identified to be overexpressed in breast cancer tissues compared with that in the peritumoral breast tissues. In addition, the knockdown of Talin2 by specific RNA interference markedly inhibited cell growth, and caused the downregulation of the apoptotic markers, cleaved Caspase‑3 and phosphorylation of poly ADP‑ribose polymerase. These findings demonstrate that Talin2 expression is upregulated in human breast cancer and that downregulation of Talin2 may serve as a useful therapeutic target in patients with breast cancer.

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