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Pro‑apoptotic effects of Kangfuxin on human stomach cancer cells and its underlying mechanism

  • Authors:
    • Xiuying Ma
    • Jia Sun
    • Weijian Ye
    • Yewei Huang
    • Congcong Sun
    • Youli Tao
    • Tao Wang
    • Weitao Cong
    • Funeng Geng
  • View Affiliations / Copyright

    Affiliations: Sichuan Key Laboratory of Medical American Cockroach, Chengdu, Sichuan 610000, P.R. China, College of Pharmacy, Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 931-939
    |
    Published online on: May 16, 2018
       https://doi.org/10.3892/ol.2018.8713
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Abstract

Kangfuxin (KFX) is an oral liquid derived from Periplaneta americana, with complex components. KFX has been demonstrated to exhibit anticancer activity in a variety of different types of tumor, including gastric cancer; however, its underlying molecular mechanism remains unclear. The present study was designed to investigate the pro‑apoptotic effects of KFX on SGC‑7901 cells, in order to provide a theoretical basis for clinical application. In order to clarify the pro‑apoptotic effects of KFX on SGC‑7901 cells, MTT analysis was conducted. To evaluate the anticancer effect of KFX, peroxisome proliferator‑activated receptor (PPAR)‑γ was analyzed by reverse transcription‑polymerase chain reaction. Western blot analysis was used to determine the effects of KFX on the expression of cleaved caspase‑3, phosphorylated extracellular signal‑regulated kinase (p‑ERK), ERK, tumor protein p53 (p53), B‑cell lymphoma 2 (Bcl‑2), Bcl‑2 associated X, interleukin (IL)‑6 and IL‑1β. In addition, terminal deoxynucleotidyl‑transferase‑mediated dUTP nick‑end labeling (TUNEL) analysis was used to detect apoptosis in SGC‑7901 cells. It was revealed that PPAR‑γ was increased in SGC‑7901 cells following treatment with KFX, shown by an increase in mRNA expression. Furthermore, western blot analysis identified that KFX treatment groups exhibited markedly inhibited levels of Bcl‑2, IL‑6, IL‑1β and p‑ERK, and induced p53 protein expression. Additionally, TUNEL and MTT assays demonstrated that treatment with KFX may induce SGC‑7901 cell apoptosis and inhibit proliferation. In conclusion, to the best of our knowledge, the results of the present study demonstrated for the first time that KFX may induce SGC‑7901 cell apoptosis and inhibit its proliferation, and this may be primarily attributed to its role in mitogen‑activated protein kinase/extracellular‑signal‑regulated kinase kinase/ERK signaling pathway inhibition.
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Copy and paste a formatted citation
Spandidos Publications style
Ma X, Sun J, Ye W, Huang Y, Sun C, Tao Y, Wang T, Cong W and Geng F: Pro‑apoptotic effects of Kangfuxin on human stomach cancer cells and its underlying mechanism. Oncol Lett 16: 931-939, 2018.
APA
Ma, X., Sun, J., Ye, W., Huang, Y., Sun, C., Tao, Y. ... Geng, F. (2018). Pro‑apoptotic effects of Kangfuxin on human stomach cancer cells and its underlying mechanism. Oncology Letters, 16, 931-939. https://doi.org/10.3892/ol.2018.8713
MLA
Ma, X., Sun, J., Ye, W., Huang, Y., Sun, C., Tao, Y., Wang, T., Cong, W., Geng, F."Pro‑apoptotic effects of Kangfuxin on human stomach cancer cells and its underlying mechanism". Oncology Letters 16.1 (2018): 931-939.
Chicago
Ma, X., Sun, J., Ye, W., Huang, Y., Sun, C., Tao, Y., Wang, T., Cong, W., Geng, F."Pro‑apoptotic effects of Kangfuxin on human stomach cancer cells and its underlying mechanism". Oncology Letters 16, no. 1 (2018): 931-939. https://doi.org/10.3892/ol.2018.8713
Copy and paste a formatted citation
x
Spandidos Publications style
Ma X, Sun J, Ye W, Huang Y, Sun C, Tao Y, Wang T, Cong W and Geng F: Pro‑apoptotic effects of Kangfuxin on human stomach cancer cells and its underlying mechanism. Oncol Lett 16: 931-939, 2018.
APA
Ma, X., Sun, J., Ye, W., Huang, Y., Sun, C., Tao, Y. ... Geng, F. (2018). Pro‑apoptotic effects of Kangfuxin on human stomach cancer cells and its underlying mechanism. Oncology Letters, 16, 931-939. https://doi.org/10.3892/ol.2018.8713
MLA
Ma, X., Sun, J., Ye, W., Huang, Y., Sun, C., Tao, Y., Wang, T., Cong, W., Geng, F."Pro‑apoptotic effects of Kangfuxin on human stomach cancer cells and its underlying mechanism". Oncology Letters 16.1 (2018): 931-939.
Chicago
Ma, X., Sun, J., Ye, W., Huang, Y., Sun, C., Tao, Y., Wang, T., Cong, W., Geng, F."Pro‑apoptotic effects of Kangfuxin on human stomach cancer cells and its underlying mechanism". Oncology Letters 16, no. 1 (2018): 931-939. https://doi.org/10.3892/ol.2018.8713
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