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Article Open Access

Photodynamic diagnosis of peritoneal metastasis in human pancreatic cancer using 5‑aminolevulinic acid during staging laparoscopy

  • Authors:
    • Kyoichi Harada
    • Yasutoshi Murayama
    • Hidemasa Kubo
    • Hisataka Matsuo
    • Ryo Morimura
    • Hisashi Ikoma
    • Hitoshi Fujiwara
    • Kazuma Okamoto
    • Tohru Tanaka
    • Eigo Otsuji
  • View Affiliations / Copyright

    Affiliations: Division of Digestive Surgery, Department of Surgery, Kyoto Prefectural University of Medicine, Kyoto 602‑8566, Japan, SBI Pharmaceuticals Co., Ltd., Izumi Garden Tower 20F, Tokyo 106‑6020, Japan
    Copyright: © Harada et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 821-828
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    Published online on: May 17, 2018
       https://doi.org/10.3892/ol.2018.8732
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Abstract

Peritoneal metastasis is an important prognostic factor for pancreatic cancer. The present study evaluated the possibility of diagnosing peritoneal metastasis by a photodynamic diagnosis using 5‑aminolevulinic acid (5‑ALA‑PDD). In vitro, protoporphyrin IX (PpIX) accumulation was examined in the AsPC‑1‑GFP cell line following 5‑ALA hydrochloride administration. In vivo, AsPC‑1‑GFP cells were injected into the peritoneal cavities of mice. Three weeks later 5‑ALA hydrochloride was intraperitoneally administered to the mice. The peritoneal nodules were observed under fluorescence excitation. A total of 34 patients were enrolled in the present study who were clinically diagnosed with pancreatic malignancy. 5‑ALA hydrochloride was orally administered to the patients prior to surgery. During the operation the abdominal cavity was observed under white light and fluorescence. In vitro and in vivo, it was confirmed that PpIX‑induced red fluorescence. In 9 patients peritoneal nodules suspected to be peritoneal metastasis were observed under white light. In 4 of the 9 patients nodules were detected on the basis of the fluorescence images. Fluorescent nodules were histopathologically diagnosed as metastatic. In the present study it was confirmed that 5‑ALA‑PDD holds promise for the rapid diagnosis of peritoneal metastasis in patients with pancreatic cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Harada K, Murayama Y, Kubo H, Matsuo H, Morimura R, Ikoma H, Fujiwara H, Okamoto K, Tanaka T, Otsuji E, Otsuji E, et al: Photodynamic diagnosis of peritoneal metastasis in human pancreatic cancer using 5‑aminolevulinic acid during staging laparoscopy. Oncol Lett 16: 821-828, 2018.
APA
Harada, K., Murayama, Y., Kubo, H., Matsuo, H., Morimura, R., Ikoma, H. ... Otsuji, E. (2018). Photodynamic diagnosis of peritoneal metastasis in human pancreatic cancer using 5‑aminolevulinic acid during staging laparoscopy. Oncology Letters, 16, 821-828. https://doi.org/10.3892/ol.2018.8732
MLA
Harada, K., Murayama, Y., Kubo, H., Matsuo, H., Morimura, R., Ikoma, H., Fujiwara, H., Okamoto, K., Tanaka, T., Otsuji, E."Photodynamic diagnosis of peritoneal metastasis in human pancreatic cancer using 5‑aminolevulinic acid during staging laparoscopy". Oncology Letters 16.1 (2018): 821-828.
Chicago
Harada, K., Murayama, Y., Kubo, H., Matsuo, H., Morimura, R., Ikoma, H., Fujiwara, H., Okamoto, K., Tanaka, T., Otsuji, E."Photodynamic diagnosis of peritoneal metastasis in human pancreatic cancer using 5‑aminolevulinic acid during staging laparoscopy". Oncology Letters 16, no. 1 (2018): 821-828. https://doi.org/10.3892/ol.2018.8732
Copy and paste a formatted citation
x
Spandidos Publications style
Harada K, Murayama Y, Kubo H, Matsuo H, Morimura R, Ikoma H, Fujiwara H, Okamoto K, Tanaka T, Otsuji E, Otsuji E, et al: Photodynamic diagnosis of peritoneal metastasis in human pancreatic cancer using 5‑aminolevulinic acid during staging laparoscopy. Oncol Lett 16: 821-828, 2018.
APA
Harada, K., Murayama, Y., Kubo, H., Matsuo, H., Morimura, R., Ikoma, H. ... Otsuji, E. (2018). Photodynamic diagnosis of peritoneal metastasis in human pancreatic cancer using 5‑aminolevulinic acid during staging laparoscopy. Oncology Letters, 16, 821-828. https://doi.org/10.3892/ol.2018.8732
MLA
Harada, K., Murayama, Y., Kubo, H., Matsuo, H., Morimura, R., Ikoma, H., Fujiwara, H., Okamoto, K., Tanaka, T., Otsuji, E."Photodynamic diagnosis of peritoneal metastasis in human pancreatic cancer using 5‑aminolevulinic acid during staging laparoscopy". Oncology Letters 16.1 (2018): 821-828.
Chicago
Harada, K., Murayama, Y., Kubo, H., Matsuo, H., Morimura, R., Ikoma, H., Fujiwara, H., Okamoto, K., Tanaka, T., Otsuji, E."Photodynamic diagnosis of peritoneal metastasis in human pancreatic cancer using 5‑aminolevulinic acid during staging laparoscopy". Oncology Letters 16, no. 1 (2018): 821-828. https://doi.org/10.3892/ol.2018.8732
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