Open Access

Knockdown of FUT3 disrupts the proliferation, migration, tumorigenesis and TGF‑β induced EMT in pancreatic cancer cells

  • Authors:
    • Ling Zhan
    • Lianyu Chen
    • Zhen Chen
  • View Affiliations

  • Published online on: May 18, 2018     https://doi.org/10.3892/ol.2018.8738
  • Pages: 924-930
  • Copyright: © Zhan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Fucosyltransferases (FUTs) are critical for glycoproteins and glycolipid chains and serve an important role in the adhesive interaction between selectins and their ligands, which contribute to tumor cell spread and metastasis. While multiple cancer cell lines heavily express FUT3, the present study investigated the expression level of FUT3 in different human pancreatic cancer cell lines. Forced expression and knockdown of FUT3 in different pancreatic cancer cell line demonstrated that FUT3 is important in cell proliferation. Using wound healing and transwell assays, it was observed that the migratory ability was decreased in FUT3 downregulated Capan‑1 cell line, compared with the normal Capan‑1 cell line. Furthermore, it was demonstrated that the knockdown of FUT3 impaired the adhesion of Capan‑1 with E‑selectin and inhibited transforming growth factor (TGF)‑β‑induced epithelial‑mesenchymal transition. These data suggest that the knockdown of FUT3 inhibits the tumorigenesis in vivo and FUT3 may be a promising target aiming at reducing the metastatic virulence of pancreatic cancer cells.
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July-2018
Volume 16 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Zhan L, Chen L and Chen Z: Knockdown of FUT3 disrupts the proliferation, migration, tumorigenesis and TGF‑β induced EMT in pancreatic cancer cells. Oncol Lett 16: 924-930, 2018
APA
Zhan, L., Chen, L., & Chen, Z. (2018). Knockdown of FUT3 disrupts the proliferation, migration, tumorigenesis and TGF‑β induced EMT in pancreatic cancer cells. Oncology Letters, 16, 924-930. https://doi.org/10.3892/ol.2018.8738
MLA
Zhan, L., Chen, L., Chen, Z."Knockdown of FUT3 disrupts the proliferation, migration, tumorigenesis and TGF‑β induced EMT in pancreatic cancer cells". Oncology Letters 16.1 (2018): 924-930.
Chicago
Zhan, L., Chen, L., Chen, Z."Knockdown of FUT3 disrupts the proliferation, migration, tumorigenesis and TGF‑β induced EMT in pancreatic cancer cells". Oncology Letters 16, no. 1 (2018): 924-930. https://doi.org/10.3892/ol.2018.8738