Mitogen‑activated protein kinase inhibition enhances the antitumor effects of sporamin in human pancreatic cancer cells

Qian,Cui‑Juan; Qi,Yong‑Xiao; Zhong,Sheng; Zeng,Ju‑Ping; Chen,Xiao‑Ying; Yao,Jun

doi:10.3892/ol.2018.8746

July-2018 Volume 16 Issue 1

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July-2018 Volume 16 Issue 1

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Article

Mitogen‑activated protein kinase inhibition enhances the antitumor effects of sporamin in human pancreatic cancer cells

Authors:
- Cui‑Juan Qian
- Yong‑Xiao Qi
- Sheng Zhong
- Ju‑Ping Zeng
- Xiao‑Ying Chen
- Jun Yao
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Affiliations: Department of Gastroenterology, Taizhou Central Hospital, Taizhou University Hospital, Taizhou, Zhejiang 318000, P.R. China, Institute of Tumor, School of Medicine, Taizhou University, Taizhou, Zhejiang 318000, P.R. China
Pages: 1237-1242
|
Published online on: May 18, 2018

https://doi.org/10.3892/ol.2018.8746
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Abstract

Sporamin, a sweet potato tuber storage protein, is a Kunitz‑type trypsin inhibitor (TI) that has exhibited antitumor activity through poorly defined mechanisms in a number of types of tumor cells. The present study aimed to analyze the combined effects of sporamin and three mitogen‑activated protein kinase (MAPK) inhibitors, PD98059, SP600125 and SB203580, on the pancreatic cancer cell line, PANC‑1. Cell proliferation activity was assessed using a 3H‑thymidine incorporation assay, and cell viability was analyzed using an MTT assay. Apoptosis was assayed by flow cytometry and fluorescence microscopy. Protein expression levels in PANC‑1 cells were determined by western blotting. The results of this analysis demonstrated that sporamin induced a temporary increase in the phosphorylation of MAPKs, including phosphorylated extracellular signal regulated‑kinase 1/2, phosphorylated c‑Jun amino‑terminal protein kinase 1/2 and phosphorylated p38‑MAPK, in a concentration‑dependent manner. However, treatment with MAPK inhibitors promoted the inhibition of cell proliferation and viability, and the induction of apoptosis in sporamin‑treated PANC‑1 cells. In conclusion, the present study demonstrated that MAPK inhibition enhanced the antitumor activity of sporamin in PANC‑1 cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Mitogen‑activated protein kinase inhibition enhances the antitumor effects of sporamin in human pancreatic cancer cells

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