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Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis

  • Authors:
    • Xuechun Leng
    • Xiangxiang Ding
    • Siwei Wang
    • Tian Fang
    • Wenrong Shen
    • Wenjia Xia
    • Ran You
    • Keping Xu
    • Rong Yin
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China, Department of Radiology, Cancer Institute of Jiangsu, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, Jiangsu 210009, P.R. China, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Department of Radiology, Cancer Institute of Jiangsu, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, Jiangsu 210009, P.R. China, Department of Comparative Medicine, Jingling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, P.R. China
    Copyright: © Leng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 727-732
    |
    Published online on: May 22, 2018
       https://doi.org/10.3892/ol.2018.8784
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Abstract

Long noncoding RNA (lncRNA) has been indicated to have an important role in various types of malignant tumors; however, only a small number of lncRNAs have been entirely elucidated. In the present study, a novel lncRNA, actin filament associated protein 1 antisense RNA 1 (AFAP1‑AS1), was investigated, which is highly expressed in non‑small cell lung cancer (NSCLC). Reverse transcription‑quantitative polymerase chain reaction and in situ hybridization were performed to detect AFAP1‑AS1 expression in frozen tissues and tissue microarrays, respectively. The results revealed that the expression level of AFAP1‑AS1 was significantly increased in tumor tissues, compared with the paired non‑cancerous tissues. It was also determined that the AFAP1‑AS1 expression level was higher in patients with lymph node metastasis than those without lymph node metastasis (P=0.014). Kaplan‑Meier analysis was conducted to evaluate the overall survival of patients with NSCLC and different expression levels of AFAP1‑AS1, and the results indicated that patients with high AFAP1‑AS1 expression had a reduced survival time, compared with those with low AFAP1‑AS1 expression (P=0.011). Cox regression analysis was also performed to analyze the prognostic value of lncRNA AFAP1‑AS1. The obtained data demonstrated that lncRNA AFAP1‑AS1 was an unfavorable prognostic biomarker for NSCLC (HR: 3.12, 95% CI (1.05‑9.25), P=0.040). In conclusion, it was demonstrated that lncRNA AFAP1‑AS1 is overexpressed in NSCLC and an unfavorable biomarker for patients with NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Leng X, Ding X, Wang S, Fang T, Shen W, Xia W, You R, Xu K and Yin R: Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis. Oncol Lett 16: 727-732, 2018.
APA
Leng, X., Ding, X., Wang, S., Fang, T., Shen, W., Xia, W. ... Yin, R. (2018). Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis. Oncology Letters, 16, 727-732. https://doi.org/10.3892/ol.2018.8784
MLA
Leng, X., Ding, X., Wang, S., Fang, T., Shen, W., Xia, W., You, R., Xu, K., Yin, R."Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis". Oncology Letters 16.1 (2018): 727-732.
Chicago
Leng, X., Ding, X., Wang, S., Fang, T., Shen, W., Xia, W., You, R., Xu, K., Yin, R."Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis". Oncology Letters 16, no. 1 (2018): 727-732. https://doi.org/10.3892/ol.2018.8784
Copy and paste a formatted citation
x
Spandidos Publications style
Leng X, Ding X, Wang S, Fang T, Shen W, Xia W, You R, Xu K and Yin R: Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis. Oncol Lett 16: 727-732, 2018.
APA
Leng, X., Ding, X., Wang, S., Fang, T., Shen, W., Xia, W. ... Yin, R. (2018). Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis. Oncology Letters, 16, 727-732. https://doi.org/10.3892/ol.2018.8784
MLA
Leng, X., Ding, X., Wang, S., Fang, T., Shen, W., Xia, W., You, R., Xu, K., Yin, R."Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis". Oncology Letters 16.1 (2018): 727-732.
Chicago
Leng, X., Ding, X., Wang, S., Fang, T., Shen, W., Xia, W., You, R., Xu, K., Yin, R."Long noncoding RNA AFAP1‑AS1 is upregulated in NSCLC and associated with lymph node metastasis and poor prognosis". Oncology Letters 16, no. 1 (2018): 727-732. https://doi.org/10.3892/ol.2018.8784
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