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MiR-1256 suppresses proliferation and migration of non-small cell lung cancer via regulating TCTN1

  • Authors:
    • Wei Liu
    • Xiuwei Wan
    • Zongyun Mu
    • Fei Li
    • Lei Wang
    • Jing Zhao
    • Xiaori Huang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, People's Hospital of Rizhao, Rizhao, Shandong 276826, P.R. China
  • Pages: 1708-1714
    |
    Published online on: May 24, 2018
       https://doi.org/10.3892/ol.2018.8794
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Abstract

Mounting evidence has shown that miRNA expression is abnormal in various human cancers. Here, we mainly explored the biological function and the potential mechanisms of miR-1256 in non-small cell lung cancer (NSCLC). The miR-1256 mRNA expression was detected by quantitative real-time PCR and tectonic family member 1 (TCTN1) mRNA expression was detected by immunoblotting. The TCTN1 was identified to be the direct and specific target gene of miR-1256 by luciferase reporter assay. Cell proliferation was examined by methyl thiazolyl tetrazolium assay and migration was detected by transwell assay. MiR-1256 expression was downregulated in NSCLC tissues, whereas the expression of TCTN1 was upregulated, compared with normal tissues. We also found that overexpression of miR-1256 in these NSCLC cell lines inhibited cell proliferation and migration. Furthermore, TCTN1 was identified as a direct target of miR-1256 by luciferase reporter assays. Collectively, these data stated that the inhibitory effect of miR-1256 in NSCLC was realized by upregulating TCTN1, suggesting that miR-1256/TCTN1 axis may play a critical role as NSCLC therapeutic target.
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Copy and paste a formatted citation
Spandidos Publications style
Liu W, Wan X, Mu Z, Li F, Wang L, Zhao J and Huang X: MiR-1256 suppresses proliferation and migration of non-small cell lung cancer via regulating TCTN1. Oncol Lett 16: 1708-1714, 2018.
APA
Liu, W., Wan, X., Mu, Z., Li, F., Wang, L., Zhao, J., & Huang, X. (2018). MiR-1256 suppresses proliferation and migration of non-small cell lung cancer via regulating TCTN1. Oncology Letters, 16, 1708-1714. https://doi.org/10.3892/ol.2018.8794
MLA
Liu, W., Wan, X., Mu, Z., Li, F., Wang, L., Zhao, J., Huang, X."MiR-1256 suppresses proliferation and migration of non-small cell lung cancer via regulating TCTN1". Oncology Letters 16.2 (2018): 1708-1714.
Chicago
Liu, W., Wan, X., Mu, Z., Li, F., Wang, L., Zhao, J., Huang, X."MiR-1256 suppresses proliferation and migration of non-small cell lung cancer via regulating TCTN1". Oncology Letters 16, no. 2 (2018): 1708-1714. https://doi.org/10.3892/ol.2018.8794
Copy and paste a formatted citation
x
Spandidos Publications style
Liu W, Wan X, Mu Z, Li F, Wang L, Zhao J and Huang X: MiR-1256 suppresses proliferation and migration of non-small cell lung cancer via regulating TCTN1. Oncol Lett 16: 1708-1714, 2018.
APA
Liu, W., Wan, X., Mu, Z., Li, F., Wang, L., Zhao, J., & Huang, X. (2018). MiR-1256 suppresses proliferation and migration of non-small cell lung cancer via regulating TCTN1. Oncology Letters, 16, 1708-1714. https://doi.org/10.3892/ol.2018.8794
MLA
Liu, W., Wan, X., Mu, Z., Li, F., Wang, L., Zhao, J., Huang, X."MiR-1256 suppresses proliferation and migration of non-small cell lung cancer via regulating TCTN1". Oncology Letters 16.2 (2018): 1708-1714.
Chicago
Liu, W., Wan, X., Mu, Z., Li, F., Wang, L., Zhao, J., Huang, X."MiR-1256 suppresses proliferation and migration of non-small cell lung cancer via regulating TCTN1". Oncology Letters 16, no. 2 (2018): 1708-1714. https://doi.org/10.3892/ol.2018.8794
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