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Article

Downregulation of long non‑coding RNA GAS5 promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma

Retraction in: /10.3892/ol.2024.14372
  • Authors:
    • Ke Ke
    • Zhanwen Sun
    • Zhengjun Wang
  • View Affiliations / Copyright

    Affiliations: Department of Head and Neck (Esophagus) Medical Oncology, Huangshi Central Hospital of Edong Healthcare Group, Affiliated Hospital of Hubei Polytechnic University, Huangshi, Hubei 435000, P.R. China, Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention, Huangshi Central Hospital of Edong Healthcare Group, Affiliated Hospital of Hubei Polytechnic University, Huangshi, Hubei 435000, P.R. China
  • Pages: 1801-1808
    |
    Published online on: May 24, 2018
       https://doi.org/10.3892/ol.2018.8797
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Abstract

The present study aimed to investigate the potential role of long non‑coding RNA growth arrest‑specific transcript 5 (lncRNA GAS5) in the progression of esophageal squamous cell carcinoma (ESCC) and to reveal its possible regulatory mechanism. The expression of lncRNA GAS5 in ESCC tissues and cell lines was analyzed using reverse transcription‑quantitative polymerase chain reaction and western blot analysis. The overexpression vector pc‑GAS5 and control vector pc‑negative control (NC), containing no GAS5 sequence, were transfected into ESCC cells. The effects of lncRNA GAS5 overexpression on cell proliferation, cell cycle distribution, cell migration and invasion were then analyzed. Besides, the expression levels of ATM‑CHK2 pathway‑associated proteins and epithelial‑mesenchymal transition (EMT)‑associated proteins were measured. Expression of lncRNA GAS5 was downregulated in the ESCC tissues compared with adjacent normal tissues, and was also downregulated in ESCC Kyse450 cells compared with the human esophageal epithelial HET‑1A cell line. Additionally, lncRNA GAS5 was successfully overexpressed in ESCC cells following transfection with pc‑GAS5. Overexpression of lncRNA GAS5 significantly inhibited cell proliferation, induced cell cycle arrest at G2/M phase and suppressed cellular migration and invasion. When cells were transfected with pc‑GAS5, the levels of phosphorylated (p)‑ATM serine/threonine protein kinase, p‑checkpoint kinase 2 (CHK2), p‑cell division cycle 25C, p‑cyclin‑dependent kinase 1, N‑cadherin, vimentin and Snail were significantly increased, whereas that of E‑cadherin were markedly decreased. The results of the present study indicate that overexpression of lncRNA GAS5 may inhibit cell proliferation, migration and invasion in ESCC. lncRNA GAS5 overexpression may induce cell cycle arrest at G2/M stage by activating the ATM‑CHK2 pathway. The results of the current study further indicate that lncRNA GAS5 overexpression may suppress cell migration and invasion via EMT‑associated proteins. lncRNA GAS5 could therefore serve as a potential target for ESCC therapy.
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1 

Siegel RL, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Holmes RS and Vaughan TL: Epidemiology and pathogenesis of esophageal cancer. Semin Radiat Oncol. 17:2–9. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Enzinger PC and Mayer RJ: Esophageal cancer. N Engl J Med. 349:2241–2252. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Hu D, Zhang M, Wang S and Wang Z: High expression of cyclooxygenase 2 is an indicator of prognosis for patients with esophageal squamous cell carcinoma after Ivor Lewis esophagectomy. Thorac Cancer. 7:310–315. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Qin HD, Liao XY, Chen YB, Huang SY, Xue WQ, Li FF, Ge XS, Liu DQ, Cai Q, Long J, et al: Genomic characterization of esophageal squamous cell carcinoma reveals critical genes underlying tumorigenesis and poor prognosis. Am J Hum Genet. 98:709–727. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Gibb EA, Brown CJ and Wan LL: The functional role of long non-coding RNA in human carcinomas. Mol Cancer. 10:382011. View Article : Google Scholar : PubMed/NCBI

7 

Cheetham SW, Gruhl F, Mattick JS and Dinger ME: Long noncoding RNAs and the genetics of cancer. Br J Cancer. 108:2419–2425. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Yao J, Huang JX, Lin M, Wu ZD, Yu H, Wang PC, Ye J, Chen P, Wu J and Zhao GJ: Microarray expression profile analysis of aberrant long non-coding RNAs in esophageal squamous cell carcinoma. Int J Oncol. 48:2543–2557. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Chen X, Kong J, Ma Z, Gao S and Feng X: Up regulation of the long non-coding RNA NEAT1 promotes esophageal squamous cell carcinoma cell progression and correlates with poor prognosis. Am J Cancer Res. 5:2808–2815. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Tan D, Wu Y, Hu L, He P, Xiong G, Bai Y and Yang K: Long noncoding RNA H19 is up-regulated in esophageal squamous cell carcinoma and promotes cell proliferation and metastasis. Dis Esophagus. 30:1–9. 2017.

11 

Zang W, Wang T, Wang Y, Chen X, Du Y, Sun Q, Li M, Dong Z and Zhao G: Knockdown of long non-coding RNA TP73-AS1 inhibits cell proliferation and induces apoptosis in esophageal squamous cell carcinoma. Oncotarget. 7:19960–19974. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Schneider C, King RM and Philipson L: Genes specifically expressed at growth arrest of mammalian cells. Cell. 54:787–793. 1988. View Article : Google Scholar : PubMed/NCBI

13 

Smith CM and Steitz JA: Classification of gas5 as a multi-small-nucleolar-RNA (snoRNA) host gene and a member of the 5′-terminal oligopyrimidine gene family reveals common features of snoRNA host genes. Mol Cell Biol. 18:6897–6909. 1998. View Article : Google Scholar : PubMed/NCBI

14 

Pickard MR, Mourtada-Maarabouni M and Williams GT: Long non-coding RNA GAS5 regulates apoptosis in prostate cancer cell lines. Biochim Biophys Acta. 1832:1613–1623. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Chang L, Li C, Lan T, Wu L, Yuan Y, Liu Q and Liu Z: Decreased expression of long non-coding RNA GAS5 indicates a poor prognosis and promotes cell proliferation and invasion in hepatocellular carcinoma by regulating vimentin. Mol Med Rep. 13:1541–1550. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Shi X, Sun M, Liu H, Yao Y, Kong R, Chen F and Song Y: A critical role for the long non-coding RNA GAS5 in proliferation and apoptosis in non-small-cell lung cancer. Mol Carcinog. 54 Suppl 1:E1–E12. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Mourtada-Maarabouni M, Pickard MR, Hedge VL, Farzaneh F and Williams GT: GAS5, a non-protein-coding RNA, controls apoptosis and is downregulated in breast cancer. Oncogene. 28:195–208. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Qiao HP, Gao WS, Huo JX and Yang ZS: Long non-coding RNA GAS5 functions as a tumor suppressor in renal cell carcinoma. Asian Pac J Cancer Prev. 14:1077–1082. 2013. View Article : Google Scholar : PubMed/NCBI

20 

García-Claver A, Lorente M, Mur P, Campos-Martín Y, Mollejo M, Velasco G and Meléndez B: Gene expression changes associated with erlotinib response in glioma cell lines. Eur J Cancer. 49:1641–1653. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Chen T, Stephens PA, Middleton FK and Curtin NJ: Targeting the S and G2 checkpoint to treat cancer. Drug Discov Today. 17:194–202. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Squatrito M, Brennan CW, Helmy K, Huse JT, Petrini JH and Holland EC: Loss of ATM/Chk2/p53 pathway components accelerates tumor development and contributes to radiation resistance in gliomas. Cancer Cell. 18:619–629. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Kim S, Lee HS, Ji JH, Cho MY, Yoo YS, Park YY, Cha HJ, Lee Y, Kim Y and Cho H: Hepatitis B virus X protein activates the ATM-Chk2 pathway and delays the cell cycle progression. J Gen Virol. 96:2242–2251. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Zhu H, Miao ZH, Huang M, Feng JM, Zhang ZX, Lu JJ, Cai YJ, Tong LJ, Xu YF, Qian XH and Ding J: Naphthalimides induce G(2) arrest through the ATM-activated Chk2-executed pathway in HCT116 cells. Neoplasia. 11:1226–1234. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Gaul L, Mandl-Weber S, Baumann P, Emmerich B and Schmidmaier R: Bendamustine induces G2 cell cycle arrest and apoptosis in myeloma cells: The role of ATM-Chk2-Cdc25A and ATM-p53-p21-pathways. J Cancer Res Clin Oncol. 134:245–253. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Zhang R, Zhu L, Zhang L, Xu A, Li Z, Xu Y, He P, Wu M, Wei F and Wang C: PTEN enhances G2/M arrest in etoposide-treated MCF-7 cells through activation of the ATM pathway. Oncol Rep. 35:2707–2714. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Yang J and Weinberg RA: Epithelial-mesenchymal transition: At the crossroads of development and tumor metastasis. Dev Cell. 14:818–829. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Thompson EW, Newgreen DF and Tarin D: Carcinoma invasion and metastasis: A role for epithelial-mesenchymal transition? Cancer Res. 65:5991–5995. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Ju JY, Yu WJ and Zhao CL: PLK1 promotes invasion of esophageal squamous cell carcinoma cells through inducing epithelial-mesenchymal transition. Adv Mater Res. 998-999:279–282. 2014. View Article : Google Scholar

30 

Ping FM, Liu GJ, Liu ZJ, Li HB, Zhai JW, Li SX, Liu YM, Li BW and Wei H: Expression of RKIP, E-cadherin and NF-kB p65 in esophageal squamous cell carcinoma and their correlations. Int J Clin Exp Pathol. 8:10164–10170. 2014.

31 

Ma B, Zhang HY, Bai X, Wang F, Ren XH, Zhang L and Zhang MZ: ADAM10 mediates the cell invasion and metastasis of human esophageal squamous cell carcinoma via regulation of E-cadherin activity. Oncol Rep. 35:2785–2794. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Natsugoe S, Uchikado Y, Okumura H, Matsumoto M, Setoyama T, Tamotsu K, Kita Y, Sakamoto A, Owaki T, Ishigami S and Aikou T: Snail plays a key role in E-cadherin-preserved esophageal squamous cell carcinoma. Oncol Rep. 17:517–523. 2007.PubMed/NCBI

33 

Li K, He W, Lin N, Wang X and Fan QX: N-cadherin knock-down decreases invasiveness of esophageal squamous cell carcinoma in vitro. World J Gastroenterol. 15:697–704. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Wang F, Li XK, Xu HY, Shan ZZ, Wang T, Yang ZC, He W, Wang LX and Fan QX: N-cadherin participated in invasion and metastasis of human esophageal squamous cell carcinoma via taking part in the formation of vasculogenic mimicry. Med Oncol. 32:4802015. View Article : Google Scholar : PubMed/NCBI

35 

Jin H, Morohashi S, Sato F, Kudo Y, Akasaka H, Tsutsumi S, Ogasawara H, Miyamoto K, Wajima N, Kawasaki H, et al: Vimentin expression of esophageal squamous cell carcinoma and its aggressive potential for lymph node metastasis. Biomed Res. 31:105–112. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ke K, Sun Z and Wang Z: Downregulation of long non‑coding RNA GAS5 promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma Retraction in /10.3892/ol.2024.14372. Oncol Lett 16: 1801-1808, 2018.
APA
Ke, K., Sun, Z., & Wang, Z. (2018). Downregulation of long non‑coding RNA GAS5 promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma Retraction in /10.3892/ol.2024.14372. Oncology Letters, 16, 1801-1808. https://doi.org/10.3892/ol.2018.8797
MLA
Ke, K., Sun, Z., Wang, Z."Downregulation of long non‑coding RNA GAS5 promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma Retraction in /10.3892/ol.2024.14372". Oncology Letters 16.2 (2018): 1801-1808.
Chicago
Ke, K., Sun, Z., Wang, Z."Downregulation of long non‑coding RNA GAS5 promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma Retraction in /10.3892/ol.2024.14372". Oncology Letters 16, no. 2 (2018): 1801-1808. https://doi.org/10.3892/ol.2018.8797
Copy and paste a formatted citation
x
Spandidos Publications style
Ke K, Sun Z and Wang Z: Downregulation of long non‑coding RNA GAS5 promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma Retraction in /10.3892/ol.2024.14372. Oncol Lett 16: 1801-1808, 2018.
APA
Ke, K., Sun, Z., & Wang, Z. (2018). Downregulation of long non‑coding RNA GAS5 promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma Retraction in /10.3892/ol.2024.14372. Oncology Letters, 16, 1801-1808. https://doi.org/10.3892/ol.2018.8797
MLA
Ke, K., Sun, Z., Wang, Z."Downregulation of long non‑coding RNA GAS5 promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma Retraction in /10.3892/ol.2024.14372". Oncology Letters 16.2 (2018): 1801-1808.
Chicago
Ke, K., Sun, Z., Wang, Z."Downregulation of long non‑coding RNA GAS5 promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma Retraction in /10.3892/ol.2024.14372". Oncology Letters 16, no. 2 (2018): 1801-1808. https://doi.org/10.3892/ol.2018.8797
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