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Association of the characteristics of B‑ and T‑cell repertoires with papillary thyroid carcinoma

  • Authors:
    • Guoping Sun
    • Lumei Qiu
    • Zhiqiang Cheng
    • Weibing Pan
    • Jingjun Qiu
    • Chang Zou
    • Ni Xie
    • Song Liu
    • Peng Zhu
    • Jun Zeng
    • Yong Dai
  • View Affiliations / Copyright

    Affiliations: Core Laboratory, Pingshan New District People's Hospital, Shenzhen, Guangdong 518118, P.R. China, Clinical Medical Research Center, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China, Core Laboratory, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1584-1592
    |
    Published online on: May 24, 2018
       https://doi.org/10.3892/ol.2018.8800
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Abstract

Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. Complementarity‑determining region 3 (CDR3) of B‑cell receptors (BCRs) and T‑cell ­receptors are the major site of antigen recognition, which determines a unique clone type, and are considered to be the ­representative of the disease. In the present study, high‑throughput sequencing was used to analyze the association of ­characteristics of the BCR immunoglobulin heavy chain (IGH) and the T‑cell receptor β chain (TRB) CDR3 genes in PTC and corresponding pericarcinous tissues from patients. A difference of CDR3 length distributions of total IGH CDR3 sequences between the two groups was revealed. IGHV3‑11/IGHJ6, TRBV2/TRBJ1‑2 and TRBV2/TRBJ1‑1 may be biomarkers for the development of PTC. Furthermore, it was revealed that the extent of the common clonotype expressions at the amino acid level was slightly higher compared with the nucleotide level. The Shannon entropy demonstrated a diversity reduction in PTC compared with the pericarcinous group, and the highly expended clone (HEC) expression of PTC was higher compared with that of the corresponding pericarcinous group. Additionally, the highest clone frequency percentage of IGH and TRB was at 0.1‑1.0% degree of expansion, as HEC expression was higher in PTC compared with the matched group. There was no shared clone of HECs in the two groups either at the amino acid level or at the nucleotide expression level. The differential expression of CDR3 sequences of PTC have been identified in the present study. Further research is required for assessing the immune repertoire size, diversity, cloning tracking and finding public clones of T‑cell and B‑cell populations in the development of PTC.
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Copy and paste a formatted citation
Spandidos Publications style
Sun G, Qiu L, Cheng Z, Pan W, Qiu J, Zou C, Xie N, Liu S, Zhu P, Zeng J, Zeng J, et al: Association of the characteristics of B‑ and T‑cell repertoires with papillary thyroid carcinoma. Oncol Lett 16: 1584-1592, 2018.
APA
Sun, G., Qiu, L., Cheng, Z., Pan, W., Qiu, J., Zou, C. ... Dai, Y. (2018). Association of the characteristics of B‑ and T‑cell repertoires with papillary thyroid carcinoma. Oncology Letters, 16, 1584-1592. https://doi.org/10.3892/ol.2018.8800
MLA
Sun, G., Qiu, L., Cheng, Z., Pan, W., Qiu, J., Zou, C., Xie, N., Liu, S., Zhu, P., Zeng, J., Dai, Y."Association of the characteristics of B‑ and T‑cell repertoires with papillary thyroid carcinoma". Oncology Letters 16.2 (2018): 1584-1592.
Chicago
Sun, G., Qiu, L., Cheng, Z., Pan, W., Qiu, J., Zou, C., Xie, N., Liu, S., Zhu, P., Zeng, J., Dai, Y."Association of the characteristics of B‑ and T‑cell repertoires with papillary thyroid carcinoma". Oncology Letters 16, no. 2 (2018): 1584-1592. https://doi.org/10.3892/ol.2018.8800
Copy and paste a formatted citation
x
Spandidos Publications style
Sun G, Qiu L, Cheng Z, Pan W, Qiu J, Zou C, Xie N, Liu S, Zhu P, Zeng J, Zeng J, et al: Association of the characteristics of B‑ and T‑cell repertoires with papillary thyroid carcinoma. Oncol Lett 16: 1584-1592, 2018.
APA
Sun, G., Qiu, L., Cheng, Z., Pan, W., Qiu, J., Zou, C. ... Dai, Y. (2018). Association of the characteristics of B‑ and T‑cell repertoires with papillary thyroid carcinoma. Oncology Letters, 16, 1584-1592. https://doi.org/10.3892/ol.2018.8800
MLA
Sun, G., Qiu, L., Cheng, Z., Pan, W., Qiu, J., Zou, C., Xie, N., Liu, S., Zhu, P., Zeng, J., Dai, Y."Association of the characteristics of B‑ and T‑cell repertoires with papillary thyroid carcinoma". Oncology Letters 16.2 (2018): 1584-1592.
Chicago
Sun, G., Qiu, L., Cheng, Z., Pan, W., Qiu, J., Zou, C., Xie, N., Liu, S., Zhu, P., Zeng, J., Dai, Y."Association of the characteristics of B‑ and T‑cell repertoires with papillary thyroid carcinoma". Oncology Letters 16, no. 2 (2018): 1584-1592. https://doi.org/10.3892/ol.2018.8800
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