Open Access

12‑Lipoxygenase promotes invasion and metastasis of human gastric cancer cells via epithelial‑mesenchymal transition

  • Authors:
    • Canmei Zhong
    • Mingkai Zhuang
    • Xiazhong Wang
    • Jianying Li
    • Zhixin Chen
    • Yuehong Huang
    • Fenglin Chen
  • View Affiliations

  • Published online on: May 24, 2018     https://doi.org/10.3892/ol.2018.8808
  • Pages: 1455-1462
  • Copyright: © Zhong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The role of 12‑lipoxygenase (12‑LOX) in tumorigenesis has been well established in several types of human cancer, including gastric cancer. It was reported that epithelial‑mesenchymal transition (EMT) contributes to tumor invasion and metastasis. However, whether 12‑LOX promotes the invasion and metastasis of human gastric cancer cells via EMT remains to be elucidated. In the present study, the expression of 12‑LOX and EMT markers, N‑cadherin and E‑cadherin, was evaluated in gastric cancer and adjacent normal mucosa samples by immunohistochemical analysis. 12‑LOX‑overexpressing gastric cancer cells were established via lentiviral transfection of SCG‑7901 cells. Wound‑healing and Transwell assays were performed to examine the regulation of cell metastasis and invasion by 12‑LOX. Furthermore, the regulation of N‑cadherin expression by 12‑LOX was evaluated using reverse transcription‑quantitative polymerase chain reaction and western blotting. The results revealed that the expression of 12‑LOX and N‑cadherin was significantly higher in gastric cancer compared with that in adjacent normal mucosa tissues (P<0.05). By contrast, the expression of E‑cadherin was significantly decreased in gastric cancer compared with that in adjacent normal mucosa tissues (P<0.05). Furthermore, the expression of 12‑LOX was positively associated with N‑cadherin expression in gastric cancer tissues. 12‑LOX‑overexpressing gastric cancer cells exhibited significantly increased invasion and migration abilities compared with the empty vector and control groups. The expression of N‑cadherin in 12‑LOX‑overexpressing gastric cancer cells was increased compared with that in the empty vector and control groups. The present study suggests that EMT may be involved in the promotion of the invasion and metastasis of human gastric cancer cells by 12‑LOX.
View Figures
View References

Related Articles

Journal Cover

August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhong C, Zhuang M, Wang X, Li J, Chen Z, Huang Y and Chen F: 12‑Lipoxygenase promotes invasion and metastasis of human gastric cancer cells via epithelial‑mesenchymal transition. Oncol Lett 16: 1455-1462, 2018
APA
Zhong, C., Zhuang, M., Wang, X., Li, J., Chen, Z., Huang, Y., & Chen, F. (2018). 12‑Lipoxygenase promotes invasion and metastasis of human gastric cancer cells via epithelial‑mesenchymal transition. Oncology Letters, 16, 1455-1462. https://doi.org/10.3892/ol.2018.8808
MLA
Zhong, C., Zhuang, M., Wang, X., Li, J., Chen, Z., Huang, Y., Chen, F."12‑Lipoxygenase promotes invasion and metastasis of human gastric cancer cells via epithelial‑mesenchymal transition". Oncology Letters 16.2 (2018): 1455-1462.
Chicago
Zhong, C., Zhuang, M., Wang, X., Li, J., Chen, Z., Huang, Y., Chen, F."12‑Lipoxygenase promotes invasion and metastasis of human gastric cancer cells via epithelial‑mesenchymal transition". Oncology Letters 16, no. 2 (2018): 1455-1462. https://doi.org/10.3892/ol.2018.8808