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Article

Effect of Derris scandens extract on a human hepatocellular carcinoma cell line

  • Authors:
    • Duanghathai Kuljittichanok
    • Penchatr Diskul‑Na‑Ayudthaya
    • Churat Weeraphan
    • Daranee Chokchaichamnankit
    • Khajeelak Chiablaem
    • Kriengsak Lirdprapamongkol
    • Jisnuson Svasti
    • Chantragan Srisomsap
  • View Affiliations / Copyright

    Affiliations: Applied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok 10210, Thailand, Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand
  • Pages: 1943-1952
    |
    Published online on: May 29, 2018
       https://doi.org/10.3892/ol.2018.8824
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Abstract

The incidence rate of hepatocellular carcinoma (HCC) remains high in numerous countries, including Thailand. There are numerous different lines of HCC treatment; however, various side effects and the resistance of cancer cells during treatment remain issues. At present, traditionally used herb plants have been widely used as alternatives to cancer therapy. Derris scandens is a Thai traditional herb which is commonly found in Thailand and widely used as a traditional medicine for numerous different diseases. The cytotoxicity of D. scandens ethanolic extract on a HCC cell line (HCC‑S102) was determined using an MTT assay. Following treatment with D. scandens ethanolic extract, the induction of apoptosis was determined by Annexin V and dead cell assays, and then confirmed by the upregulation of cleaved poly(ADP‑ribose) polymerase. Furthermore, a proteomic approach was used in order to study protein alteration upon treatment with D. scandens ethanolic extract coupled with liquid chromatography‑tandem mass spectrometry analysis for protein identification. The results suggested that D. scandens ethanolic extract resulted in cytotoxicity against HCC‑S102 cells, as the half‑maximal inhibitory concentration values were 36.0±1.0, 29.6±0.6, and 22.6±1.5 µg/ml at 24, 48 and 72 h, respectively. Apoptotic cells were induced following treatment with D. scandens. The comparative proteomic profiles of D. scandens ethanolic extract‑treated and untreated cells revealed various protein targets for anticancer activity including heterogeneous nuclear ribonucleoprotein (hnRNP) K, hnRNP A2/B1, stomatin‑like 2 and GAPDH. In the present study, the anticancer activity of D. scandens ethanolic extract was demonstrated to affect the cell proliferation of HCC‑S102 via an apoptotic pathway. The alteration in these proteins provides a better understanding of the mechanism of action of D. scandens, which may be a promising anticancer agent for the treatment of patients with HCC in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Kuljittichanok D, Diskul‑Na‑Ayudthaya P, Weeraphan C, Chokchaichamnankit D, Chiablaem K, Lirdprapamongkol K, Svasti J and Srisomsap C: Effect of Derris scandens extract on a human hepatocellular carcinoma cell line. Oncol Lett 16: 1943-1952, 2018.
APA
Kuljittichanok, D., Diskul‑Na‑Ayudthaya, P., Weeraphan, C., Chokchaichamnankit, D., Chiablaem, K., Lirdprapamongkol, K. ... Srisomsap, C. (2018). Effect of Derris scandens extract on a human hepatocellular carcinoma cell line. Oncology Letters, 16, 1943-1952. https://doi.org/10.3892/ol.2018.8824
MLA
Kuljittichanok, D., Diskul‑Na‑Ayudthaya, P., Weeraphan, C., Chokchaichamnankit, D., Chiablaem, K., Lirdprapamongkol, K., Svasti, J., Srisomsap, C."Effect of Derris scandens extract on a human hepatocellular carcinoma cell line". Oncology Letters 16.2 (2018): 1943-1952.
Chicago
Kuljittichanok, D., Diskul‑Na‑Ayudthaya, P., Weeraphan, C., Chokchaichamnankit, D., Chiablaem, K., Lirdprapamongkol, K., Svasti, J., Srisomsap, C."Effect of Derris scandens extract on a human hepatocellular carcinoma cell line". Oncology Letters 16, no. 2 (2018): 1943-1952. https://doi.org/10.3892/ol.2018.8824
Copy and paste a formatted citation
x
Spandidos Publications style
Kuljittichanok D, Diskul‑Na‑Ayudthaya P, Weeraphan C, Chokchaichamnankit D, Chiablaem K, Lirdprapamongkol K, Svasti J and Srisomsap C: Effect of Derris scandens extract on a human hepatocellular carcinoma cell line. Oncol Lett 16: 1943-1952, 2018.
APA
Kuljittichanok, D., Diskul‑Na‑Ayudthaya, P., Weeraphan, C., Chokchaichamnankit, D., Chiablaem, K., Lirdprapamongkol, K. ... Srisomsap, C. (2018). Effect of Derris scandens extract on a human hepatocellular carcinoma cell line. Oncology Letters, 16, 1943-1952. https://doi.org/10.3892/ol.2018.8824
MLA
Kuljittichanok, D., Diskul‑Na‑Ayudthaya, P., Weeraphan, C., Chokchaichamnankit, D., Chiablaem, K., Lirdprapamongkol, K., Svasti, J., Srisomsap, C."Effect of Derris scandens extract on a human hepatocellular carcinoma cell line". Oncology Letters 16.2 (2018): 1943-1952.
Chicago
Kuljittichanok, D., Diskul‑Na‑Ayudthaya, P., Weeraphan, C., Chokchaichamnankit, D., Chiablaem, K., Lirdprapamongkol, K., Svasti, J., Srisomsap, C."Effect of Derris scandens extract on a human hepatocellular carcinoma cell line". Oncology Letters 16, no. 2 (2018): 1943-1952. https://doi.org/10.3892/ol.2018.8824
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