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Article

Aneuploidy of chromosome 8 and mutation of circulating tumor cells predict pathologic complete response in the treatment of locally advanced rectal cancer

  • Authors:
    • Jue‑Feng Wan
    • Xue‑Qin Li
    • Jing Zhang
    • Li‑Feng Yang
    • Ji Zhu
    • Gui‑Chao Li
    • Li‑Ping Liang
    • Li‑Jun Shen
    • Hui Zhang
    • Jing Li
    • Yi‑Tong Zhang
    • Chang‑Yue Chen
    • Zhen Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai 200032, P.R. China, Department of Oncology, Affiliated Hospital of Jining Medical University, Jining, Shandong 272000, P.R. China, Department of Medical Research, Shanghai MajorMed Diagnostics Company, Shanghai 201321, P.R. China
  • Pages: 1863-1868
    |
    Published online on: May 30, 2018
       https://doi.org/10.3892/ol.2018.8831
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Abstract

Identifying patients who may or may not achieve pathologic complete response (pathCR) allows for treatment with alternative approaches in the preoperative setting. The aim of the current study was to investigate whether aneuploidy of chromosome 8 and mutations of circulating tumor cells (CTCs) could predict the response of patients with rectal cancer to preoperative chemoradiotherapy. A total of 33 patients with locally advanced rectal cancer (cT3‑T4 and/or cN+) treated with neoadjuvant chemoradiotherapy between September 2014 and March 2015 were recruited. Blood samples were collected from 33 patients with pre‑chemoradiotherapy rectal cancer. It was demonstrated that ≥5 copies of chromosome 8 was associated with pathCR (univariate logistic regression, P=0.042). Of the 6 patients whose CTCs had <5 copies of chromosome 8, 3 achieved pathCR (3/6, 50%), and of the 27 patients whose CTCs had ≥5 copies of chromosome 8 obtained 3 pathCR (3/27, 11.1%; Chi‑square test, P=0.0255). Of the 33 patients with mutations assessed, 8 significant nonsynonymous mutations in CTCs were identified as associated with pathCR (Chi‑square test, P‑values range, 0.0004‑0.0298; mutations in ARID1A, HDAC1, APC, ERBB3, TP53, AMER1 and AR). These results suggest that ≥5 copies of chromosome 8 and 8 nonsynonymous mutations in ARID1A, HDAC1, APC, ERBB3, TP53, AMER1 AR in CTCs were associated with pathCR. This conclusion should be validated further in larger prospective studies and the long‑term follow‑up survival data of this study will also be reported in the future.
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1 

Aschele C, Cionini L, Lonardi S, Pinto C, Cordio S, Rosati G, Artale S, Tagliagambe A, Ambrosini G, Rosetti P, et al: Primary tumor response to preoperative chemoradiation with or without oxaliplatin in locally advanced rectal cancer: Pathologic results of the STAR-01 randomized phase III trial. J Clin Oncol. 29:2773–2780. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Gérard JP, Azria D, Gourgou-Bourgade S, Martel-Laffay I, Hennequin C, Etienne PL, Vendrely V, François E, de La Roche G, Bouche O, et al: Comparison of two neoadjuvant chemoradiotherapy regimens for locally advanced rectal cancer: Results of the phase III trial ACCORD 12/0405-Prodige 2. J Clin Oncol. 28:1638–1644. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Rödel C, Liersch T, Becker H, Fietkau R, Hohenberger W, Hothorn T, Graeven U, Arnold D, Lang-Welzenbach M, Raab HR, et al: Preoperative chemoradiotherapy and postoperative chemotherapy with fluorouracil and oxaliplatin versus fluorouracil alone in locally advanced rectal cancer: Initial results of the German CAO/ARO/AIO-04 randomised phase 3 trial. Lancet Oncol. 13:679–687. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Park IJ, You YN, Agarwal A, Skibber JM, Rodriguez-Bigas MA, Eng C, Feig BW, Das P, Krishnan S, Crane CH, et al: Neoadjuvant treatment response as an early response indicator for patients with rectal cancer. J Clin Oncol. 30:1770–1776. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Martens MH, Maas M, Heijnen LA, Lambregts DM, Leijtens JW, Stassen LP, Breukink SO, Hoff C, Belgers EJ, Melenhorst J, et al: Long-term outcome of an organ preservation program after neoadjuvant treatment for rectal cancer. J Natl Cancer Inst. 108(pii): djw1712016. View Article : Google Scholar : PubMed/NCBI

6 

Renehan AG, Malcomson L, Emsley R, Gollins S, Maw A, Myint AS, Rooney PS, Susnerwala S, Blower A, Saunders MP, et al: Watch-and-wait approach versus surgical resection after chemoradiotherapy for patients with rectal cancer (the OnCoRe project): A propensity-score matched cohort analysis. Lancet Oncol. 17:174–183. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Kalady MF, de Campos-Lobato LF, Stocchi L, Geisler DP, Dietz D, Lavery IC and Fazio VW: Predictive factors of pathologic complete response after neoadjuvant chemoradiation for rectal cancer. Ann Surg. 250:582–589. 2009.PubMed/NCBI

8 

Russo AL, Ryan DP, Borger DR, Wo JY, Szymonifka J, Liang WY, Kwak EL, Blaszkowsky LS, Clark JW, Allen JN, et al: Mutational and clinical predictors of pathologic complete response in the treatment of locally advanced rectal cancer. J Gastrointest Cancer. 45:34–39. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Zong C, Lu S, Chapman AR and Xie XS: Genome-wide detection of single-nucleotide and copy-number variations of a single human cell. Science. 338:1622–1626. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Armaghany T, Wilson JD, Chu Q and Mills G: Genetic alterations in colorectal cancer. Gastrointest Cancer Res. 5:19–27. 2012.PubMed/NCBI

11 

Kaz AM and Brentnall TA: Genetic testing for colon cancer. Nat Clin Pract Gastroenterol Hepatol. 3:670–679. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Martin M: Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet J. 17:10–12. 2011. View Article : Google Scholar

13 

Li H and Durbin R: Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics. 25:1754–1760. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G and Durbin R; Genome Project Data Processing S: The sequence alignment/map format and SAMtools. Bioinformatics. 25:2078–2079. 2009. View Article : Google Scholar : PubMed/NCBI

15 

DePristo MA, Banks E, Poplin R, Garimella KV, Maguire JR, Hartl C, Philippakis AA, del Angel G, Rivas MA, Hanna M, et al: A framework for variation discovery and genotyping using next-generation DNA sequencing data. Nature Genet. 43:491–498. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Leung ML, Wang Y, Waters J and Navin NE: SNES: Single nucleus exome sequencing. Genome Biol. 16:552015. View Article : Google Scholar : PubMed/NCBI

17 

Gibson MK, Abraham SC, Wu TT, Burtness B, Heitmiller RF, Heath E and Forastiere A: Epidermal growth factor receptor, p53 mutation and pathological response predict survival in patients with locally advanced esophageal cancer treated with preoperative chemoradiotherapy. Clin Cancer Res. 9:6461–6468. 2003.PubMed/NCBI

18 

Villafranca E, Okruzhnov Y, Dominguez MA, García-Foncillas J, Azinovic I, Martinez E, Illarramendi JJ, Arias F, Martinez Monge R, Salgado E, et al: Polymorphisms of the repeated sequences in the enhancer region of the thymidylate synthase gene promoter may predict downstaging after preoperative chemoradiation in rectal cancer. J Clin Oncol. 19:1779–1786. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Hao JJ, Yao HQ, Dai GY, Kang W, Jia XM, Xu X, Cai Y, Zhan QM, Wang GQ and Wang MR: Chromosomal aneuploidies and combinational fluorescence in situ hybridization probe panels are useful for predicting prognosis for esophageal squamous cell carcinoma. J Gastroenterol. 50:155–166. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Li Y, Zhang X, Ge S, Gao J, Gong J, Lu M, Zhang Q, Cao Y, Wang DD, Lin PP, et al: Clinical significance of phenotyping and karyotyping of circulating tumor cells in patients with advanced gastric cancer. Oncotarget. 5:6594–6602. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Gao Y, Zhu Y, Zhang Z, Zhang C, Huang X and Yuan Z: Clinical significance of pancreatic circulating tumor cells using combined negative enrichment and immunostaining-fluorescence in situ hybridization. J Exp Clin Cancer Res. 35:662016. View Article : Google Scholar : PubMed/NCBI

22 

Wu C, Hao H, Li L, Zhou X, Guo Z, Zhang L, Zhang X, Zhong W, Guo H, Bremner RM, et al: Preliminary investigation of the clinical significance of detecting circulating tumor cells enriched from lung cancer patients. J Thorac Oncol. 4:30–36. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Chen Z, Liu Z, Li W, Qu K, Deng X, Varma MG, Fichera A, Pigazzi A and Garcia-Aguilar J: Chromosomal copy number alterations are associated with tumor response to chemoradiation in locally advanced rectal cancer. Genes Chromosomes Cancer. 50:689–699. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Dumont P, Leu JI, Della Pietra AC III, George DL and Murphy M: The codon 72 polymorphic variants of p53 have markedly different apoptotic potential. Nat Genet. 33:357–365. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Johnstone RW, Ruefli AA and Lowe SW: Apoptosis: A link between cancer genetics and chemotherapy. Cell. 108:153–164. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Lin PP: Integrated EpCAM-independent subtraction enrichment and iFISH strategies to detect and classify disseminated and circulating tumors cells. Clin Transl Med. 4:382015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wan JF, Li XQ, Zhang J, Yang LF, Zhu J, Li GC, Liang LP, Shen LJ, Zhang H, Li J, Li J, et al: Aneuploidy of chromosome 8 and mutation of circulating tumor cells predict pathologic complete response in the treatment of locally advanced rectal cancer. Oncol Lett 16: 1863-1868, 2018.
APA
Wan, J., Li, X., Zhang, J., Yang, L., Zhu, J., Li, G. ... Zhang, Z. (2018). Aneuploidy of chromosome 8 and mutation of circulating tumor cells predict pathologic complete response in the treatment of locally advanced rectal cancer. Oncology Letters, 16, 1863-1868. https://doi.org/10.3892/ol.2018.8831
MLA
Wan, J., Li, X., Zhang, J., Yang, L., Zhu, J., Li, G., Liang, L., Shen, L., Zhang, H., Li, J., Zhang, Y., Chen, C., Zhang, Z."Aneuploidy of chromosome 8 and mutation of circulating tumor cells predict pathologic complete response in the treatment of locally advanced rectal cancer". Oncology Letters 16.2 (2018): 1863-1868.
Chicago
Wan, J., Li, X., Zhang, J., Yang, L., Zhu, J., Li, G., Liang, L., Shen, L., Zhang, H., Li, J., Zhang, Y., Chen, C., Zhang, Z."Aneuploidy of chromosome 8 and mutation of circulating tumor cells predict pathologic complete response in the treatment of locally advanced rectal cancer". Oncology Letters 16, no. 2 (2018): 1863-1868. https://doi.org/10.3892/ol.2018.8831
Copy and paste a formatted citation
x
Spandidos Publications style
Wan JF, Li XQ, Zhang J, Yang LF, Zhu J, Li GC, Liang LP, Shen LJ, Zhang H, Li J, Li J, et al: Aneuploidy of chromosome 8 and mutation of circulating tumor cells predict pathologic complete response in the treatment of locally advanced rectal cancer. Oncol Lett 16: 1863-1868, 2018.
APA
Wan, J., Li, X., Zhang, J., Yang, L., Zhu, J., Li, G. ... Zhang, Z. (2018). Aneuploidy of chromosome 8 and mutation of circulating tumor cells predict pathologic complete response in the treatment of locally advanced rectal cancer. Oncology Letters, 16, 1863-1868. https://doi.org/10.3892/ol.2018.8831
MLA
Wan, J., Li, X., Zhang, J., Yang, L., Zhu, J., Li, G., Liang, L., Shen, L., Zhang, H., Li, J., Zhang, Y., Chen, C., Zhang, Z."Aneuploidy of chromosome 8 and mutation of circulating tumor cells predict pathologic complete response in the treatment of locally advanced rectal cancer". Oncology Letters 16.2 (2018): 1863-1868.
Chicago
Wan, J., Li, X., Zhang, J., Yang, L., Zhu, J., Li, G., Liang, L., Shen, L., Zhang, H., Li, J., Zhang, Y., Chen, C., Zhang, Z."Aneuploidy of chromosome 8 and mutation of circulating tumor cells predict pathologic complete response in the treatment of locally advanced rectal cancer". Oncology Letters 16, no. 2 (2018): 1863-1868. https://doi.org/10.3892/ol.2018.8831
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