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Article

Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin

  • Authors:
    • Fengyu Huang
    • Peitao Wang
    • Xinsheng Wang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Medicine, Medical College of Qingdao University, Qingdao, Shandong 266021, P.R. China, Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266011, P.R. China
  • Pages: 1975-1980
    |
    Published online on: May 30, 2018
       https://doi.org/10.3892/ol.2018.8833
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Abstract

It is widely considered that endoplasmic reticulum stress may rapidly induce apoptosis. The aim of the present study was to investigate the effect of thapsigargin on the induction of apoptosis in prostate cancer cells, and to explore its possible mechanism. A Cell Counting Kit‑8 was selected to determine the effect of thapsigargin (0, 1, 10 and 100 nM) on the proliferation of PC3 cells. Cell proliferation of the prostate cancer cells was effectively inhibited by treatment with thapsigargin, and thapsigargin significantly increased the rate of apoptosis and caspase‑3/9 activities in prostate cancer cells. The protein expression of phosphorylated (p)‑RAC‑α serine threonine‑protein kinase, p‑mechanistic target of rapamycin, F‑actin and paxillin were significantly decreased, and cofilin‑1 protein expression was significantly increased by treatment with thapsigargin in prostate cancer cells. Overall, the data of the present study revealed that thapsigargin induced apoptosis in prostate cancer cells through cofilin‑1 and paxillin.
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1 

D'Amico AV, Chen MH, Renshaw A, Loffredo M and Kantoff PW: Long-term follow-up of a randomized trial of radiation with or without androgen deprivation therapy for localized prostate cancer. JAMA. 314:1291–1293. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Fedorov A, Fluckiger J, Ayers GD, Li X, Gupta SN, Tempany C, Mulkern R, Yankeelov TE and Fennessy FM: A comparison of two methods for estimating DCE-MRI parameters via individual and cohort based AIFs in prostate cancer: A step towards practical implementation. Magn Reson Imaging. 32:321–329. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Yu EY, Massard C, Gross ME, Carducci MA, Culine S, Hudes G, Posadas EM, Sternberg CN, Wilding G, Trudel GC, et al: Once-daily dasatinib: Expansion of phase II study evaluating safety and efficacy of dasatinib in patients with metastatic castration-resistant prostate cancer. Urology. 77:1166–1171. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Houédé N, Pulido M, Mourey L, Joly F, Ferrero JM, Bellera C, Priou F, Lalet C, Laroche-Clary A, Raffin MC, et al: A phase II trial evaluating the efficacy and safety of efavirenz in metastatic castration-resistant prostate cancer. Oncologist. 19:1227–1228. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Thomas R, Williams M, Sharma H, Chaudry A and Bellamy P: A double-blind, placebo-controlled randomised trial evaluating the effect of a polyphenol-rich whole food supplement on PSA progression in men with prostate cancer-the U.K. NCRN Pomi-T study. Prostate Cancer Prostatic Dis. 17:180–186. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Obakan P, Arisan ED, Coker-Gurkan A and Palavan-Unsal N: Epibrassinolide-induced apoptosis regardless of p53 expression via activating polyamine catabolic machinery, a common target for androgen sensitive and insensitive prostate cancer cells. Prostate. 74:1622–1633. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Reshma RS, Sreelatha KH, Somasundaram V, Satheesh Kumar S, Nadhan R, Nair RS and Srinivas P: Plumbagin, a naphthaquinone derivative induces apoptosis in BRCA 1/2 defective castrate resistant prostate cancer cells as well as prostate cancer stem-like cells. Pharmacol Res. 105:134–145. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Kwegyir-Afful AK, Ramalingam S, Purushottamachar P, Ramamurthy VP and Njar VC: Galeterone and VNPT55 induce proteasomal degradation of AR/AR-V7, induce significant apoptosis via cytochrome c release and suppress growth of castration resistant prostate cancer xenografts in vivo. Oncotarget. 6:27440–27460. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Zhu B, Fukada K, Zhu H and Kyprianou N: Prohibitin and cofilin are intracellular effectors of transforming growth factor beta signaling in human prostate cancer cells. Cancer Res. 66:8640–8647. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Lu LI, Fu NI, Luo XU, Li XY and Li XP: Overexpression of cofilin 1 in prostate cancer and the corresponding clinical implications. Oncol Lett. 9:2757–2761. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Sundram V, Chauhan SC, Ebeling M and Jaggi M: Curcumin attenuates β-catenin signaling in prostate cancer cells through activation of protein kinase D1. PLoS One. 7:e353682012. View Article : Google Scholar : PubMed/NCBI

12 

Hammes SR, Miedlich SU and Sen A: Paxillin and steroid signaling: From frog to human. Methods Mol Biol. 1204:95–108. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Bokobza SM, Ye L, Kynaston HG and Jiang WG: Growth and differentiation factor-9 promotes adhesive and motile capacity of prostate cancer cells by up-regulating FAK and Paxillin via Smad dependent pathway. Oncol Rep. 24:1653–1659. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Janyou A, Changtam C, Suksamrarn A, Tocharus C and Tocharus J: Suppression effects of O-demethyldemethoxycurcumin on thapsigargin triggered on endoplasmic reticulum stress in SK-N-SH cells. Neurotoxicology. 50:92–100. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Drexler HC: Synergistic apoptosis induction in leukemic cells by the phosphatase inhibitor salubrinal and proteasome inhibitors. PLoS One. 4:e41612009. View Article : Google Scholar : PubMed/NCBI

16 

Muramatsu Y, Maemoto T, Iwashita A and Matsuoka N: Novel neuroprotective compound SCH-20148 rescues thymocytes and SH-SY5Y cells from thapsigargin-induced mitochondrial membrane potential reduction and cell death. Eur J Pharmacol. 563:40–48. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Wolpin BM, O'Reilly EM, Ko YJ, Blaszkowsky LS, Rarick M, Rocha-Lima CM, Ritch P, Chan E, Spratlin J, Macarulla T, et al: Global, multicenter, randomized, phase II trial of gemcitabine and gemcitabine plus AGS-1C4D4 in patients with previously untreated, metastatic pancreatic cancer. Ann Oncol. 24:1792–1801. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Matsumoto K, Hagiwara M, Tanaka N, Hayakawa N, Ishida M, Ninomiya A, Nakajima Y and Nakamura S: Survival following primary androgen deprivation therapy for localized intermediate- or high-risk prostate cancer: Comparison with the life expectancy of the age-matched normal population. Med Oncol. 31:9792014. View Article : Google Scholar : PubMed/NCBI

19 

Cella D, Ivanescu C, Holmstrom S, Bui CN, Spalding J and Fizazi K: Impact of enzalutamide on quality of life in men with metastatic castration-resistant prostate cancer after chemotherapy: Additional analyses from the AFFIRM randomized clinical trial. Ann Oncol. 26:179–185. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Kato M, Banuelos CA, Imamura Y, Leung JK, Caley DP, Wang J, Mawji NR and Sadar MD: Cotargeting androgen receptor splice variants and mTOR signaling pathway for the treatment of castration-resistant prostate cancer. Clin Cancer Res. 22:2744–2754. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Fang F and Wang L, Zhang S, Fang Q, Hao F, Sun Y, Zhao L, Chen S, Liao H and Wang L: CD147 modulates autophagy through the PI3K/Akt/mTOR pathway in human prostate cancer PC-3 cells. Oncol Lett. 9:1439–1443. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Chiu LY, Hu ME, Yang TY, Hsin IL, Ko JL, Tsai KJ and Sheu GT: Immunomodulatory protein from ganoderma microsporum induces pro-death autophagy through Akt-mTOR-p70S6K pathway inhibition in multidrug resistant lung cancer cells. PLoS One. 10:e01257742015. View Article : Google Scholar : PubMed/NCBI

23 

Asahara S, Shibutani Y, Teruyama K, Inoue HY, Kawada Y, Etoh H, Matsuda T, Kimura-Koyanagi M, Hashimoto N, Sakahara M, et al: Ras-related C3 botulinum toxin substrate 1 (RAC1) regulates glucose-stimulated insulin secretion via modulation of F-actin. Diabetologia. 56:1088–1097. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Yu Y, Yang O, Fazli L, Rennie PS, Gleave ME and Dong X: Progesterone receptor expression during prostate cancer progression suggests a role of this receptor in stromal cell differentiation. Prostate. 75:1043–1050. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Luo Y, Cui X, Zhao J, Han Y, Li M, Lin Y, Jiang Y and Lan L: Cells susceptible to epithelial-mesenchymal transition are enriched in stem-like side population cells from prostate cancer. Oncol Rep. 31:874–884. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Yip KH, Zheng MH, Steer JH, Giardina TM, Han R, Lo SZ, Bakker AJ, Cassady AI, Joyce DA and Xu J: Thapsigargin modulates osteoclastogenesis through the regulation of RANKL-induced signaling pathways and reactive oxygen species production. J Bone Miner Res. 20:1462–1471. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Wang F, Liu DZ, Xu H, Li Y, Wang W, Liu BL and Zhang LY: Thapsigargin induces apoptosis by impairing cytoskeleton dynamics in human lung adenocarcinoma cells. ScientificWorldJournal. 2014:6190502014.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Huang F, Wang P and Wang X: Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin. Oncol Lett 16: 1975-1980, 2018.
APA
Huang, F., Wang, P., & Wang, X. (2018). Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin. Oncology Letters, 16, 1975-1980. https://doi.org/10.3892/ol.2018.8833
MLA
Huang, F., Wang, P., Wang, X."Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin". Oncology Letters 16.2 (2018): 1975-1980.
Chicago
Huang, F., Wang, P., Wang, X."Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin". Oncology Letters 16, no. 2 (2018): 1975-1980. https://doi.org/10.3892/ol.2018.8833
Copy and paste a formatted citation
x
Spandidos Publications style
Huang F, Wang P and Wang X: Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin. Oncol Lett 16: 1975-1980, 2018.
APA
Huang, F., Wang, P., & Wang, X. (2018). Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin. Oncology Letters, 16, 1975-1980. https://doi.org/10.3892/ol.2018.8833
MLA
Huang, F., Wang, P., Wang, X."Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin". Oncology Letters 16.2 (2018): 1975-1980.
Chicago
Huang, F., Wang, P., Wang, X."Thapsigargin induces apoptosis of prostate cancer through cofilin‑1 and paxillin". Oncology Letters 16, no. 2 (2018): 1975-1980. https://doi.org/10.3892/ol.2018.8833
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