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RNA binding protein HuR promotes osteosarcoma cell progression via suppressing the miR‑142‑3p/HMGA1 axis

  • Authors:
    • Weicheng Pan
    • Jinhui Pang
    • Bin Ji
    • Zhen Wang
    • Chengwei Liu
    • Yan Cheng
    • Lei Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Shanghai Putuo District Central Hospital, Shanghai 200062, P.R. China
    Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1475-1482
    |
    Published online on: May 31, 2018
       https://doi.org/10.3892/ol.2018.8855
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Abstract

The present study aimed to study the roles and underlying mechanisms of human antigen R (HuR) in osteosarcoma (OS) cell progression. It was determined that the HuR mRNA and protein levels were significantly upregulated in OS tissues, compared with that in normal adjacent tissues. HuR expression was negatively associated with miR‑142‑3p expression, but positively with High Mobility Group AT‑Hook 1 (HMGA1). Additionally, knockdown of HuR inhibited OS cells viability, epithelial‑mesenchymal transition and promoted cell apoptosis. HuR was determined to harbor binding sites on HMGA1, directly binding to HMGA1, increasing HMGA1 mRNA stability and expression. Notably, the promotion of HuR on HMGA1 expression was attenuated via miR‑142‑3p overexpression, and miR‑142‑3p could directly bind to HMGA1 3'untranslated region (UTR). Furthermore, HMGA1 3'UTR with a mutated miR‑142‑3p binding site did not respond to HuR alterations. Finally, the inhibition of HuR knockdown was attenuated or even reversed via HMGA1 overexpression; therefore, the results of the present study indicated that RNA binding protein HuR may facilitate OS cell progression via competitively binding to HMGA1 with miR‑142‑3p.
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Copy and paste a formatted citation
Spandidos Publications style
Pan W, Pang J, Ji B, Wang Z, Liu C, Cheng Y and Zhang L: RNA binding protein HuR promotes osteosarcoma cell progression via suppressing the miR‑142‑3p/HMGA1 axis. Oncol Lett 16: 1475-1482, 2018.
APA
Pan, W., Pang, J., Ji, B., Wang, Z., Liu, C., Cheng, Y., & Zhang, L. (2018). RNA binding protein HuR promotes osteosarcoma cell progression via suppressing the miR‑142‑3p/HMGA1 axis. Oncology Letters, 16, 1475-1482. https://doi.org/10.3892/ol.2018.8855
MLA
Pan, W., Pang, J., Ji, B., Wang, Z., Liu, C., Cheng, Y., Zhang, L."RNA binding protein HuR promotes osteosarcoma cell progression via suppressing the miR‑142‑3p/HMGA1 axis". Oncology Letters 16.2 (2018): 1475-1482.
Chicago
Pan, W., Pang, J., Ji, B., Wang, Z., Liu, C., Cheng, Y., Zhang, L."RNA binding protein HuR promotes osteosarcoma cell progression via suppressing the miR‑142‑3p/HMGA1 axis". Oncology Letters 16, no. 2 (2018): 1475-1482. https://doi.org/10.3892/ol.2018.8855
Copy and paste a formatted citation
x
Spandidos Publications style
Pan W, Pang J, Ji B, Wang Z, Liu C, Cheng Y and Zhang L: RNA binding protein HuR promotes osteosarcoma cell progression via suppressing the miR‑142‑3p/HMGA1 axis. Oncol Lett 16: 1475-1482, 2018.
APA
Pan, W., Pang, J., Ji, B., Wang, Z., Liu, C., Cheng, Y., & Zhang, L. (2018). RNA binding protein HuR promotes osteosarcoma cell progression via suppressing the miR‑142‑3p/HMGA1 axis. Oncology Letters, 16, 1475-1482. https://doi.org/10.3892/ol.2018.8855
MLA
Pan, W., Pang, J., Ji, B., Wang, Z., Liu, C., Cheng, Y., Zhang, L."RNA binding protein HuR promotes osteosarcoma cell progression via suppressing the miR‑142‑3p/HMGA1 axis". Oncology Letters 16.2 (2018): 1475-1482.
Chicago
Pan, W., Pang, J., Ji, B., Wang, Z., Liu, C., Cheng, Y., Zhang, L."RNA binding protein HuR promotes osteosarcoma cell progression via suppressing the miR‑142‑3p/HMGA1 axis". Oncology Letters 16, no. 2 (2018): 1475-1482. https://doi.org/10.3892/ol.2018.8855
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