Antioxidant activity and leukemia initiation prevention in vitro and in vivo by N‑acetyl‑L‑cysteine
- Wen‑Ling Shih
- Ching‑Dong Chang
- Hsiu‑Tsu Chen
- Kuo‑Kuang Fan
Affiliations: Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, R.O.C., Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, R.O.C., Department of Fashion Design and Management, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan, R.O.C., Graduate School of Design, National Yunlin University of Science and Technology, Yunlin 64002, Taiwan, R.O.C.
- Published online on: May 31, 2018 https://doi.org/10.3892/ol.2018.8864
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N‑acetyl‑L‑cysteine (NAC) is the most abundant water‑soluble component of garlic. No study to date has studied the leukemia prevention ability of NAC in mouse systemic leukemia model. The current study aimed to investigate the leukemia initiation prevention potential of NAC in a mouse model. The cytotoxic concentration of NAC was determined first in HL‑60 cells, and its in vivo activity was studied in a mouse acute myelocytic leukemia model with WEHI‑3 leukemia cells. The results showed that a non‑toxic concentration of NAC efficiently scavenged free‑radicals, lowered lipid peroxidation and reduced DNA damage induced by hydrogen peroxide in a cultured HL‑60 leukemia cell line. NAC also elevated the cellular antioxidant enzyme activity significantly. Furthermore, NAC prevented mouse death induced by injection of murine WEHI‑3 leukemia cells and reduced organ damage, as well as activated antioxidant mechanisms. The results of this study provided strong evidence that NAC may have potential benefits in terms of elevating antioxidant activity and preventing leukemia initiation.