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Article Open Access

miR‑224/miR‑141 ratio as a novel diagnostic biomarker in renal cell carcinoma

  • Authors:
    • Xuanyu Chen
    • Ning Lou
    • Anming Ruan
    • Bin Qiu
    • Yun Yan
    • Xuegang Wang
    • Quansheng Du
    • Hailong Ruan
    • Weiwei Han
    • Haibin Wei
    • Hongmei Yang
    • Xiaoping Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China, Department of Gastroenterology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China, Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA 30912, USA, Department of Urology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China, Department of Pathogen Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1666-1674
    |
    Published online on: June 1, 2018
       https://doi.org/10.3892/ol.2018.8874
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Abstract

Biomarkers to guide the clinical treatment of patients with renal cell carcinoma (RCC) are not yet routinely available. MicroRNAs (miRNAs) have been demonstrated to serve as biomarkers for a number of types of cancer. Based on a previous study by this group, we hypothesize that several highly differentially expressed miRNAs may serve as tissue and plasma biomarkers in patients with RCC. The expression levels of miR‑210, miR‑224 and miR‑141 were analyzed in tissue samples from the same cohort of 78 patients with RCC, in paired pre‑ and post‑operative plasma samples from 66 patients with clear cell RCC (ccRCC) and in 67 healthy controls by reverse transcription‑quantitative polymerase chain reaction. Receiver operating characteristic (ROC) was used to evaluate the diagnostic accuracy associated with the expression of miR‑210, miR‑224 and miR‑141. ROC curves revealed that the diagnostic accuracy (area under the curve) of tissue miR‑210, miR‑224, the ratio of miR‑210/miR‑141 (miR210/141), miR‑224/miR‑141 (miR224/141) and miR‑210x miR‑224/miR‑141 (miR210x224/141) in ccRCC was 0.8329, 0.8511, 0.9412, 0.9898 and 0.9771, respectively. Notably, miR224/141 demonstrated the highest accuracy among these miRNAs for discriminating ccRCC tissues from normal tissues, with a sensitivity of 97.06% and a specificity of 98.53%. The expression levels of plasma miR‑210 and miR‑224 were significantly increased in patients compared with healthy control patients, and were reduced postoperatively (P<0.05). The diagnostic accuracy of plasma miR‑210 and miR‑224 were 0.6775 (89.55% sensitivity and 48.48% specificity) and 0.6056 (88.06% sensitivity and 40.91% specificity), respectively. The present study indicated that the tissue miR‑224/miR‑141 ratio is a potentially powerful tool for detecting ccRCC. However, plasma miR‑210 and miR‑224 may not be associated with diagnosis of ccRCC.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Lou N, Ruan A, Qiu B, Yan Y, Wang X, Du Q, Ruan H, Han W, Wei H, Wei H, et al: miR‑224/miR‑141 ratio as a novel diagnostic biomarker in renal cell carcinoma. Oncol Lett 16: 1666-1674, 2018.
APA
Chen, X., Lou, N., Ruan, A., Qiu, B., Yan, Y., Wang, X. ... Zhang, X. (2018). miR‑224/miR‑141 ratio as a novel diagnostic biomarker in renal cell carcinoma. Oncology Letters, 16, 1666-1674. https://doi.org/10.3892/ol.2018.8874
MLA
Chen, X., Lou, N., Ruan, A., Qiu, B., Yan, Y., Wang, X., Du, Q., Ruan, H., Han, W., Wei, H., Yang, H., Zhang, X."miR‑224/miR‑141 ratio as a novel diagnostic biomarker in renal cell carcinoma". Oncology Letters 16.2 (2018): 1666-1674.
Chicago
Chen, X., Lou, N., Ruan, A., Qiu, B., Yan, Y., Wang, X., Du, Q., Ruan, H., Han, W., Wei, H., Yang, H., Zhang, X."miR‑224/miR‑141 ratio as a novel diagnostic biomarker in renal cell carcinoma". Oncology Letters 16, no. 2 (2018): 1666-1674. https://doi.org/10.3892/ol.2018.8874
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Lou N, Ruan A, Qiu B, Yan Y, Wang X, Du Q, Ruan H, Han W, Wei H, Wei H, et al: miR‑224/miR‑141 ratio as a novel diagnostic biomarker in renal cell carcinoma. Oncol Lett 16: 1666-1674, 2018.
APA
Chen, X., Lou, N., Ruan, A., Qiu, B., Yan, Y., Wang, X. ... Zhang, X. (2018). miR‑224/miR‑141 ratio as a novel diagnostic biomarker in renal cell carcinoma. Oncology Letters, 16, 1666-1674. https://doi.org/10.3892/ol.2018.8874
MLA
Chen, X., Lou, N., Ruan, A., Qiu, B., Yan, Y., Wang, X., Du, Q., Ruan, H., Han, W., Wei, H., Yang, H., Zhang, X."miR‑224/miR‑141 ratio as a novel diagnostic biomarker in renal cell carcinoma". Oncology Letters 16.2 (2018): 1666-1674.
Chicago
Chen, X., Lou, N., Ruan, A., Qiu, B., Yan, Y., Wang, X., Du, Q., Ruan, H., Han, W., Wei, H., Yang, H., Zhang, X."miR‑224/miR‑141 ratio as a novel diagnostic biomarker in renal cell carcinoma". Oncology Letters 16, no. 2 (2018): 1666-1674. https://doi.org/10.3892/ol.2018.8874
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