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Article Open Access

Hub genes and key pathways of non‑small lung cancer identified using bioinformatics

  • Authors:
    • Qing Tang
    • Hongmei Zhang
    • Man Kong
    • Xiaoli Mao
    • Xiaocui Cao
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Tongji Hospital, Wuhan, Hubei 430014, P.R. China, Department of Clinical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, P.R. China
    Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2344-2354
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    Published online on: June 4, 2018
       https://doi.org/10.3892/ol.2018.8882
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Abstract

Non‑small cell lung cancer (NSCLC) is the most common type of lung cancer, accounting for ~80% of all lung cancer cases. The aim of the present study was to identify key genes and pathways in NSCLC, in order to improve understanding of the mechanism of lung cancer. The GSE33532 gene expression dataset, containing 20 normal and 80 NSCLC samples, was used. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to obtain the enrichment data of differently expressed genes (DEGs). Disease modules within NSCLC were constructed by Cytoscape, using protein‑protein interaction (PPI) from the Search Tool for the Retrieval of Interacting Genes database. In addition, the Kaplan Meier plotter KMplot was used to assess the top hub genes in the PPI network. As a result, 1,795 genes were identified in NSCLC; 729 were upregulated and 1,066 were downregulated. The results of the GO analysis indicated that the upregulated DEGs were significantly enriched in ‘biological processes’ (BP), including ‘cell cycle and nuclear division’; the downregulated DEGs were also significantly enriched in BP, including ‘response to wounding’, ‘anatomical structure morphogenesis’ and ‘response to stimulus’. Upregulated DEGs were also enriched in ‘cell cycle’, ‘DNA replication’ and the ‘tumor protein 53 signaling pathway’, while the downregulated DEGs were also enriched in ‘complement and coagulation cascades’, ‘malaria’ and ‘cell adhesion molecules’. The top 9 hub genes were cyclin‑dependent kinase 9 (CDK1), polo‑like kinase 1, aurora kinase B, cell division cycle 20, baculoviral initiator of apoptosis repeat containing 5, mitotic checkpoint serine/threonine kinase B, proliferating cell nuclear antigen (PCNA), centromere protein A and MAD2 mitotic arrest deficient‑like 1, and the KMplot results revealed that the high expression levels of these genes resulted in significantly low survival rates, compared with low expression samples (P<0.05), with the exception of PCNA and CDK1. In the pathway crosstalk analysis, 26 nodes and 41 interactions were divided into two groups: One module of the two groups primarily included ‘metabolism of amino acid’ and the other primarily contained ‘tumor necrosis signaling’ pathways. In conclusion, the present study assisted in improving the understanding of the molecular mechanisms underlying NSCLC development, and the results may help the understanding of the biological mechanism of NSCLC.
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Spandidos Publications style
Tang Q, Zhang H, Kong M, Mao X and Cao X: Hub genes and key pathways of non‑small lung cancer identified using bioinformatics. Oncol Lett 16: 2344-2354, 2018.
APA
Tang, Q., Zhang, H., Kong, M., Mao, X., & Cao, X. (2018). Hub genes and key pathways of non‑small lung cancer identified using bioinformatics. Oncology Letters, 16, 2344-2354. https://doi.org/10.3892/ol.2018.8882
MLA
Tang, Q., Zhang, H., Kong, M., Mao, X., Cao, X."Hub genes and key pathways of non‑small lung cancer identified using bioinformatics". Oncology Letters 16.2 (2018): 2344-2354.
Chicago
Tang, Q., Zhang, H., Kong, M., Mao, X., Cao, X."Hub genes and key pathways of non‑small lung cancer identified using bioinformatics". Oncology Letters 16, no. 2 (2018): 2344-2354. https://doi.org/10.3892/ol.2018.8882
Copy and paste a formatted citation
x
Spandidos Publications style
Tang Q, Zhang H, Kong M, Mao X and Cao X: Hub genes and key pathways of non‑small lung cancer identified using bioinformatics. Oncol Lett 16: 2344-2354, 2018.
APA
Tang, Q., Zhang, H., Kong, M., Mao, X., & Cao, X. (2018). Hub genes and key pathways of non‑small lung cancer identified using bioinformatics. Oncology Letters, 16, 2344-2354. https://doi.org/10.3892/ol.2018.8882
MLA
Tang, Q., Zhang, H., Kong, M., Mao, X., Cao, X."Hub genes and key pathways of non‑small lung cancer identified using bioinformatics". Oncology Letters 16.2 (2018): 2344-2354.
Chicago
Tang, Q., Zhang, H., Kong, M., Mao, X., Cao, X."Hub genes and key pathways of non‑small lung cancer identified using bioinformatics". Oncology Letters 16, no. 2 (2018): 2344-2354. https://doi.org/10.3892/ol.2018.8882
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