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Article

Juglone suppresses epithelial‑mesenchymal transition in prostate cancer cells via the protein kinase B/glycogen synthase kinase‑3β/Snail signaling pathway

  • Authors:
    • Fang Fang
    • Shuang Chen
    • Jing Ma
    • Jiabo Cui
    • Qiang Li
    • Guixian Meng
    • Liguo Wang
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, Jilin Medical University, Jilin City, Jilin 132013, P.R. China, Department of Urology Surgery, Affiliated Hospital of Jilin Medical University, Jilin City, Jilin 132013, P.R. China
  • Pages: 2579-2584
    |
    Published online on: June 4, 2018
       https://doi.org/10.3892/ol.2018.8885
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Abstract

Epithelial‑mesenchymal transition (EMT) serves an important role in the metastasis of prostate cancer. Juglone is a natural compound isolated from plants that is reported to possess potent cytotoxic properties. However, there are no studies on the anti‑EMT effect of juglone in prostate cancer, or its potential underlying mechanisms of action. In the present study, the effect of juglone on the EMT of prostate cancer cells was investigated. Transwell assays were used to demonstrate that juglone inhibits the migration and invasion of the prostate cancer (PC) LNCaP and LNCaP‑AI cell lines. Results from western blot analysis demonstrated that juglone increases the expression of the epithelial marker E‑cadherin while decreasing the expression of mesenchymal markers (N‑cadherin and Vimentin) in a dose‑dependent manner. The data from the present study also revealed that juglone downregulates the expression of Snail, a repressor of E‑cadherin and an inducer of EMT. Furthermore, juglone prevented inactivation of glycogen synthase kinase‑3β (GSK‑3β), an endogenous inhibitor of Snail in a dose‑dependent manner. Lithium chloride (LiCl), a GSK‑3β inhibitor, prevented juglone‑mediated downregulation of Snail expression and upregulation of E‑cadherin. In addition, phosphorylation and subsequent activation of protein kinase B (Akt), which is known to phosphorylate GSK‑3β at serine 9 (Ser9), leading to its inhibition, were significantly decreased by juglone in LNCaP and LNCaP‑AI cells. Inhibition of the phosphatidylinositol‑4,5‑bisphosphate 3‑kinase (PI3K)/Akt pathway by LY294002 augmented juglone‑mediated GSK‑3β activity by inhibiting Ser9 phosphorylation. These findings indicated that juglone suppresses EMT via the Akt/GSK‑3β/Snail pathway, consequently decreasing the invasiveness of PC cells.
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Copy and paste a formatted citation
Spandidos Publications style
Fang F, Chen S, Ma J, Cui J, Li Q, Meng G and Wang L: Juglone suppresses epithelial‑mesenchymal transition in prostate cancer cells via the protein kinase B/glycogen synthase kinase‑3β/Snail signaling pathway. Oncol Lett 16: 2579-2584, 2018.
APA
Fang, F., Chen, S., Ma, J., Cui, J., Li, Q., Meng, G., & Wang, L. (2018). Juglone suppresses epithelial‑mesenchymal transition in prostate cancer cells via the protein kinase B/glycogen synthase kinase‑3β/Snail signaling pathway. Oncology Letters, 16, 2579-2584. https://doi.org/10.3892/ol.2018.8885
MLA
Fang, F., Chen, S., Ma, J., Cui, J., Li, Q., Meng, G., Wang, L."Juglone suppresses epithelial‑mesenchymal transition in prostate cancer cells via the protein kinase B/glycogen synthase kinase‑3β/Snail signaling pathway". Oncology Letters 16.2 (2018): 2579-2584.
Chicago
Fang, F., Chen, S., Ma, J., Cui, J., Li, Q., Meng, G., Wang, L."Juglone suppresses epithelial‑mesenchymal transition in prostate cancer cells via the protein kinase B/glycogen synthase kinase‑3β/Snail signaling pathway". Oncology Letters 16, no. 2 (2018): 2579-2584. https://doi.org/10.3892/ol.2018.8885
Copy and paste a formatted citation
x
Spandidos Publications style
Fang F, Chen S, Ma J, Cui J, Li Q, Meng G and Wang L: Juglone suppresses epithelial‑mesenchymal transition in prostate cancer cells via the protein kinase B/glycogen synthase kinase‑3β/Snail signaling pathway. Oncol Lett 16: 2579-2584, 2018.
APA
Fang, F., Chen, S., Ma, J., Cui, J., Li, Q., Meng, G., & Wang, L. (2018). Juglone suppresses epithelial‑mesenchymal transition in prostate cancer cells via the protein kinase B/glycogen synthase kinase‑3β/Snail signaling pathway. Oncology Letters, 16, 2579-2584. https://doi.org/10.3892/ol.2018.8885
MLA
Fang, F., Chen, S., Ma, J., Cui, J., Li, Q., Meng, G., Wang, L."Juglone suppresses epithelial‑mesenchymal transition in prostate cancer cells via the protein kinase B/glycogen synthase kinase‑3β/Snail signaling pathway". Oncology Letters 16.2 (2018): 2579-2584.
Chicago
Fang, F., Chen, S., Ma, J., Cui, J., Li, Q., Meng, G., Wang, L."Juglone suppresses epithelial‑mesenchymal transition in prostate cancer cells via the protein kinase B/glycogen synthase kinase‑3β/Snail signaling pathway". Oncology Letters 16, no. 2 (2018): 2579-2584. https://doi.org/10.3892/ol.2018.8885
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