Role of the tumour protein P53 gene in human cervical squamous carcinoma cells: Discussing haematopoietic cell‑specific protein 1‑associated protein X‑1‑induced survival, migration and proliferation

  • Authors:
    • Bing Qian
    • Li‑Jun Zhao
    • Fang Teng
    • Ling‑Juan Gao
    • Rong Shen
  • View Affiliations

  • Published online on: June 4, 2018     https://doi.org/10.3892/ol.2018.8886
  • Pages: 2629-2637
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Abstract

The haematopoietic cell‑specific protein 1‑associated protein X‑1 (HAX‑1), as a mitochondrial membrane protein, induces cancer progression and metastasis. The present study aimed to investigate the role of HAX‑1‑inducedsurvival, migration and proliferation of human cervical squamous carcinoma cells and to elucidate its potential molecular mechanisms. The level of HAX‑1 was examined by quantitative polymerase chain reaction and western blot analyses. The survival, migration and proliferation of the human cervical squamous carcinoma SiHa cell line were measured by the water‑soluble tetrazolium salt (WST‑1) assay, Transwell assay and 3H‑thymidine incorporation into DNA (3H‑TdR) assay, respectively. The intracellular reactive oxygen species (ROS) was estimated by the fluorescence of H2DCFDA, and the mitochondrial membrane potential was tested using a JC‑1 probe. The expression of the HAX‑1 gene was significantly increased in human cervical carcinoma tissues relative to non‑cancerous cervix tissues. Overexpression of HAX‑1 increased the survival, migration and proliferation ability of SiHa cells, decreased the production of ROS, and maintained the integrity of the mitochondrial membrane and morphology. The effect brought on these cells could be abrogated by the addition of wild‑type tumour protein P53 (p53) or carbonyl cyanide‑p‑trifluoro methoxyphenylhydrazone‑induced mitochondrial dysfunction. In summary, these data support the notion that HAX‑1 induced the survival, migration and proliferation of human cervical squamous carcinoma cells by inhibiting its downstream regulatory factor p53 in SiHa cells.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Qian B, Zhao LJ, Teng F, Gao LJ and Shen R: Role of the tumour protein P53 gene in human cervical squamous carcinoma cells: Discussing haematopoietic cell‑specific protein 1‑associated protein X‑1‑induced survival, migration and proliferation. Oncol Lett 16: 2629-2637, 2018
APA
Qian, B., Zhao, L., Teng, F., Gao, L., & Shen, R. (2018). Role of the tumour protein P53 gene in human cervical squamous carcinoma cells: Discussing haematopoietic cell‑specific protein 1‑associated protein X‑1‑induced survival, migration and proliferation. Oncology Letters, 16, 2629-2637. https://doi.org/10.3892/ol.2018.8886
MLA
Qian, B., Zhao, L., Teng, F., Gao, L., Shen, R."Role of the tumour protein P53 gene in human cervical squamous carcinoma cells: Discussing haematopoietic cell‑specific protein 1‑associated protein X‑1‑induced survival, migration and proliferation". Oncology Letters 16.2 (2018): 2629-2637.
Chicago
Qian, B., Zhao, L., Teng, F., Gao, L., Shen, R."Role of the tumour protein P53 gene in human cervical squamous carcinoma cells: Discussing haematopoietic cell‑specific protein 1‑associated protein X‑1‑induced survival, migration and proliferation". Oncology Letters 16, no. 2 (2018): 2629-2637. https://doi.org/10.3892/ol.2018.8886