Effects of TONS504‑photodynamic therapy on mouse mammary tumor cells

Osaki,Tomohiro; Sakata,Isao; Uto,Yoshihiro; Yamashita,Masamichi; Murahata,Yusuke; Azuma,Kazuo; Tsuka,Takeshi; Ito,Norihiko; Imagawa,Tomohiro; Okamoto,Yoshiharu

doi:10.3892/ol.2018.8887

August-2018 Volume 16 Issue 2

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August-2018 Volume 16 Issue 2

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Effects of TONS504‑photodynamic therapy on mouse mammary tumor cells

Authors:
- Tomohiro Osaki
- Isao Sakata
- Yoshihiro Uto
- Masamichi Yamashita
- Yusuke Murahata
- Kazuo Azuma
- Takeshi Tsuka
- Norihiko Ito
- Tomohiro Imagawa
- Yoshiharu Okamoto
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Affiliations: Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori 680‑8553, Japan, Porphyrin Laboratory, Okayama 700‑0086, Japan, Department of Bioengineering, Graduate School of Technology, Industrial and Social Sciences, Tokushima University, Tokushima 770‑8506, Japan

Copyright: © Osaki et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 2078-2084
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Published online on: June 4, 2018

https://doi.org/10.3892/ol.2018.8887
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Abstract

In the present study, TONS504 (C51H58N8O5I2; molecular weight, 1,116.9), a novel cationic hydrophilic photosensitizer, was synthesized from protoporphyrin IX dimethyl ester through a five‑step process according to a patented method for use in photodynamic therapy (PDT). The subcellular localization of TONS504 and the cytotoxic effects of TONS504‑mediated PDT in the mouse mammary tumor EMT6 cell line were investigated. TONS504 was localized primarily in the lysosomes and partially in the mitochondria. The cytotoxic effects of TONS504‑mediated PDT in the mouse mammary tumor EMT6 cell line were investigated using a WST8 assay and an Oxidative Stress kit. The cell viability values following treatment with 10 µg/ml TONS504 at light energies of 0, 1, 5 and 10 J/cm2 were 92.5, 101.8, 27.7 and 1.8%, respectively. The percentages of reactive oxygen species (ROS)(+) cells following the same treatment were 8.6, 8.5, 29.2 and 70.1%, respectively, whereas the percentages of apoptotic cells were 7.1, 5.6, 24.8 and 48.7%, respectively. The percentages of ROS(+) and apoptotic cells in the group subjected to TONS504‑mediated PDT increased in a manner dependent on the TONS504 concentration and light energy. Further studies are required to evaluate the in vivo pharmacokinetics, tissue distribution and photodynamic effects of TONS504.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of TONS504‑photodynamic therapy on mouse mammary tumor cells

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