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Expression of miR‑106 in endometrial carcinoma RL95‑2 cells and effect on proliferation and invasion of cancer cells

  • Authors:
    • Xingjun Li
    • Xianghua Yi
    • Chuanding Bie
    • Zhemin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Chongming Branch Hospital, Affiliated Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 202150, P.R. China, Department of Pathology, Tongji Hospital Affiliated to Tongji University, Shanghai 202150, P.R. China, Department of Pathology, Suizhou Maternal and Child Health‑Care Hospital, Suizhou, Hubei 441300, P.R. China, Department of Orthopaedics, Chongming Branch Hospital, Affiliated Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 202150, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2251-2254
    |
    Published online on: June 8, 2018
       https://doi.org/10.3892/ol.2018.8926
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Abstract

Expression of miR‑106 in endometrial carcinoma RL95‑2 cell line and its effect on proliferation and invasion of cancer cells was investigated. miR‑106 expression vector was constructed and transiently transfected into in vitro cultured RL95‑2 cells of human endometrial carcinoma. Cells were divided into three groups including blank control cells (MOCK group), miR‑106 transfection group (miR‑106 group) and negative control group (siNC group). Reverse-transcription quantitative PCR (RT‑qPCR) was used to detect the expression of miR‑106. Proliferation and in vitro migration of RL95‑2 cells were detected by MTT and scratch assay, and cell apoptosis was detected by flow cytometry. Compared with MOCK and siNC group, cell apoptosis rate was significantly decreased but cell proliferation rate was significantly increased in miR‑106 group (p<0.05). In addition, cell migration and invasion ability was significantly increased in miR‑106 group (p<0.05). Overexpression of miR‑106 can promote proliferation and inhibit apoptosis of endometrial cancer RL95‑2 cells, and miR‑106 may serve as a new target for the treatment of endometrial cancer in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Yi X, Bie C and Wang Z: Expression of miR‑106 in endometrial carcinoma RL95‑2 cells and effect on proliferation and invasion of cancer cells. Oncol Lett 16: 2251-2254, 2018.
APA
Li, X., Yi, X., Bie, C., & Wang, Z. (2018). Expression of miR‑106 in endometrial carcinoma RL95‑2 cells and effect on proliferation and invasion of cancer cells. Oncology Letters, 16, 2251-2254. https://doi.org/10.3892/ol.2018.8926
MLA
Li, X., Yi, X., Bie, C., Wang, Z."Expression of miR‑106 in endometrial carcinoma RL95‑2 cells and effect on proliferation and invasion of cancer cells". Oncology Letters 16.2 (2018): 2251-2254.
Chicago
Li, X., Yi, X., Bie, C., Wang, Z."Expression of miR‑106 in endometrial carcinoma RL95‑2 cells and effect on proliferation and invasion of cancer cells". Oncology Letters 16, no. 2 (2018): 2251-2254. https://doi.org/10.3892/ol.2018.8926
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Yi X, Bie C and Wang Z: Expression of miR‑106 in endometrial carcinoma RL95‑2 cells and effect on proliferation and invasion of cancer cells. Oncol Lett 16: 2251-2254, 2018.
APA
Li, X., Yi, X., Bie, C., & Wang, Z. (2018). Expression of miR‑106 in endometrial carcinoma RL95‑2 cells and effect on proliferation and invasion of cancer cells. Oncology Letters, 16, 2251-2254. https://doi.org/10.3892/ol.2018.8926
MLA
Li, X., Yi, X., Bie, C., Wang, Z."Expression of miR‑106 in endometrial carcinoma RL95‑2 cells and effect on proliferation and invasion of cancer cells". Oncology Letters 16.2 (2018): 2251-2254.
Chicago
Li, X., Yi, X., Bie, C., Wang, Z."Expression of miR‑106 in endometrial carcinoma RL95‑2 cells and effect on proliferation and invasion of cancer cells". Oncology Letters 16, no. 2 (2018): 2251-2254. https://doi.org/10.3892/ol.2018.8926
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