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Oxidative stress induced by carboplatin promotes apoptosis and inhibits migration of HN‑3 cells

  • Authors:
    • Pei‑Jie He
    • Rui‑Feng Ge
    • Wen‑Jing Mao
    • Phil‑Sang Chung
    • Jin‑Chul Ahn
    • Hai‑Tao Wu
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology‑Head and Neck Surgery, Shanghai Key Clinical Disciplines of Otorhinolaryngology, Eye and ENT Hospital of Fudan University, Shanghai 200031, P.R. China, Department of Otolaryngology‑Head and Neck Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China, Department of Otolaryngology‑Head and Neck Surgery, Beckman Laser Institute Korea, Dankook University, Cheonan, South Chungcheong 330‑715, Republic of Korea
    Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7131-7138
    |
    Published online on: October 10, 2018
       https://doi.org/10.3892/ol.2018.9563
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Abstract

Laryngeal squamous cell carcinoma (LSCC) is currently a serious public health problem in China; thus, it is urgent to identify effective treatment strategies for this disease. Previous studies demonstrated that reactive oxygen species (ROS) serve important roles in the apoptosis of LSCC cells. It has also been indicated that carboplatin (CBDCA), a second‑generation platinum compound with broad antineoplastic properties, is able to induce oxidative stress to produce ROS, which in turn promotes apoptosis. Thus, the present study investigated if CBDCA is cytotoxic in LSCC cells due to the oxidative stress caused by ROS. Therefore, an MTT assay was performed to determine the cell viability of HN‑3 LSCC cells following treatment with different doses of CBDCA. Subsequently, the expression levels of ROS and the rate of apoptosis/necrosis were evaluated in the cells. Following this, the HN‑3 cells were co‑treated with CBDCA and glutathione (GSH) or H2O2, followed by an MTT assay, a cell migration assay and western blot analysis. The results demonstrated that CBDCA reduced the viability of HN‑3 cells in a time‑ and dose‑dependent manner and promoted the production of ROS and apoptosis at certain doses. Additionally, the combination treatment of CBDCA and H2O2 enhanced the inhibitory effects of CBDCA on cell viability and migration ability, and promoted apoptosis in HN‑3 cells; whereas the combined treatment of CBDCA and GSH exerted opposite effects. The results of the present study demonstrated that CBDCA promotes the apoptosis of HN‑3 cells through accumulation of ROS, which may provide a novel treatment strategy for treating LSCC.
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Copy and paste a formatted citation
Spandidos Publications style
He PJ, Ge RF, Mao WJ, Chung PS, Ahn JC and Wu HT: Oxidative stress induced by carboplatin promotes apoptosis and inhibits migration of HN‑3 cells. Oncol Lett 16: 7131-7138, 2018.
APA
He, P., Ge, R., Mao, W., Chung, P., Ahn, J., & Wu, H. (2018). Oxidative stress induced by carboplatin promotes apoptosis and inhibits migration of HN‑3 cells. Oncology Letters, 16, 7131-7138. https://doi.org/10.3892/ol.2018.9563
MLA
He, P., Ge, R., Mao, W., Chung, P., Ahn, J., Wu, H."Oxidative stress induced by carboplatin promotes apoptosis and inhibits migration of HN‑3 cells". Oncology Letters 16.6 (2018): 7131-7138.
Chicago
He, P., Ge, R., Mao, W., Chung, P., Ahn, J., Wu, H."Oxidative stress induced by carboplatin promotes apoptosis and inhibits migration of HN‑3 cells". Oncology Letters 16, no. 6 (2018): 7131-7138. https://doi.org/10.3892/ol.2018.9563
Copy and paste a formatted citation
x
Spandidos Publications style
He PJ, Ge RF, Mao WJ, Chung PS, Ahn JC and Wu HT: Oxidative stress induced by carboplatin promotes apoptosis and inhibits migration of HN‑3 cells. Oncol Lett 16: 7131-7138, 2018.
APA
He, P., Ge, R., Mao, W., Chung, P., Ahn, J., & Wu, H. (2018). Oxidative stress induced by carboplatin promotes apoptosis and inhibits migration of HN‑3 cells. Oncology Letters, 16, 7131-7138. https://doi.org/10.3892/ol.2018.9563
MLA
He, P., Ge, R., Mao, W., Chung, P., Ahn, J., Wu, H."Oxidative stress induced by carboplatin promotes apoptosis and inhibits migration of HN‑3 cells". Oncology Letters 16.6 (2018): 7131-7138.
Chicago
He, P., Ge, R., Mao, W., Chung, P., Ahn, J., Wu, H."Oxidative stress induced by carboplatin promotes apoptosis and inhibits migration of HN‑3 cells". Oncology Letters 16, no. 6 (2018): 7131-7138. https://doi.org/10.3892/ol.2018.9563
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