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Article Open Access

Shikonin regulates invasion and autophagy of cultured colon cancer cells by inhibiting yes‑associated protein

  • Authors:
    • Jing Zhu
    • Lei Zhao
    • Bin Luo
    • Wang Sheng
  • View Affiliations / Copyright

    Affiliations: Laboratory of Cancer, College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, P.R. China, Reproductive Medicine Centre, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Basic Medical Sciences, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6117-6125
    |
    Published online on: October 11, 2019
       https://doi.org/10.3892/ol.2019.10980
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Abstract

Colon cancer is a common malignancy, and its morbidity and mortality have been increasing in recent years in China. Shikonin (Shi), a naturally occurring naphthoquinone, exhibits anticancer activity. However, the mechanisms of action of Shi remain unclear. The aim of the present study was to investigate the antitumor mechanism of Shi in colon cancer cells. The effects of different Shi concentrations on the viability of colon cancer cells using MTT, colony formation and wound‑healing assays were assessed. Western blot analysis was performed to detect the expression of LC3‑II, p62. Shi effectively suppressed viability and cell migration, and induced autophagy in colon cancer cells. Yes‑associated protein (YAP) increases cell viability, and inhibits cell apoptosis and cell contact. Expression of YAP is downregulated by Shi. The cytotoxic effects of Shi were further investigated on YAP overexpression and on YAP knockout cell lines. The findings revealed that Shi suppressed the viability and induced autophagy of colon cancer cells. Additionally, YAP expression reversed the effects of Shi. The results of the present study suggest that Shi may be a promising anticancer treatment for colon cancer, and YAP may be a potential diagnostic marker for colon cancer.
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1 

Jouffret L, Turrini O, Ewald J, Moutardier V, Iovanna JL and Delpero JR: Long-term survivors after pancreatectomy for cancer: The TNM classification is outdated. ANZ J Surg. 85:860–864. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

3 

DeSantis CE, Lin CC, Mariotto AB, Siegel RL, Stein KD, Kramer JL, Alteri R, Robbins AS and Jemal A: Cancer treatment and survivorship statistics, 2014. CA Cancer J Clin. 64:252–271. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Singh KE, Taylor TH, Pan CG, Stamos MJ and Zell JA: Colorectal cancer incidence among young adults in California. J Adolesc Young Adult Oncol. 3:176–184. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Wang SP and Wang LH: Disease implication of hyper-Hippo signalling. Open Biol. 6:2016. View Article : Google Scholar

6 

Wang Z, Liu P, Zhou X, Wang T, Feng X, Sun YP, Xiong Y, Yuan HX and Guan KL: Endothelin promotes colorectal tumorigenesis by activating YAP/TAZ. Cancer Res. 77:2413–2423. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Wang YP and Tang DX: Expression of Yes-associated protein in liver cancer and its correlation with clinicopathological features and prognosis of liver cancer patients. Int J Clin Exp Med. 8:1080–1086. 2015.PubMed/NCBI

8 

Wu H, Xie J, Pan Q, Wang B, Hu D and Hu X: Anticancer agent shikonin is an incompetent inducer of cancer drug resistance. PLoS One. 8:e527062013. View Article : Google Scholar : PubMed/NCBI

9 

Shi S and Cao H: Shikonin promotes autophagy in BXPC-3 human pancreatic cancer cells through the PI3K/Akt signaling pathway. Oncol Lett. 8:1087–1089. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Kim HJ, Hwang KE, Park DS, Oh SH, Jun HY, Yoon KH, Jeong ET, Kim HR and Kim YS: Shikonin-induced necroptosis is enhanced by the inhibition of autophagy in non-small cell lung cancer cells. J Transl Med. 15:1232017. View Article : Google Scholar : PubMed/NCBI

11 

Qian HR and Yang Y: Functional role of autophagy in gastric cancer. Oncotarget. 7:17641–17651. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Klionsky DJ, Abeliovich H, Agostinis P, Agrawal DK, Aliev G, Askew DS, Baba M, Baehrecke EH, Bahr BA, Ballabio A, et al: Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy. 4:151–175. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Fridlender M, Kapulnik Y and Koltai H: Plant derived substances with anti-cancer activity: From folklore to practice. Front Plant Sci. 6:7992015. View Article : Google Scholar : PubMed/NCBI

14 

Zhou W, Jiang Hda G, Peng Y and Li SS: Comparative study on enantiomeric excess of main akannin/shikonin derivatives isolated from the roots of three endemic Boraginaceae plants in China. Biomed Chromatogr. 25:1067–1075. 2011. View Article : Google Scholar : PubMed/NCBI

15 

He G, He G, Zhou R, Pi Z, Zhu T, Jiang L and Xie Y: Enhancement of cisplatin-induced colon cancer cells apoptosis by shikonin, a natural inducer of ROS in vitro and in vivo. Biochem Biophys Res Commun. 469:1075–1082. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Petherick KJ, Williams AC, Lane JD, Ordóñez-Morán P, Huelsken J, Collard TJ, Smartt HJ, Batson J, Malik K, Paraskeva C and Greenhough A: Autolysosomal β-catenin degradation regulates Wnt-autophagy-p62 crosstalk. EMBO J. 32:1903–1916. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Li YJ, Lei YH, Yao N, Wang CR, Hu N, Ye WC, Zhang DM and Chen ZS: Autophagy and multidrug resistance in cancer. Chin J Cancer. 36:522017. View Article : Google Scholar : PubMed/NCBI

18 

Xu T, Wang W, Zhang S, Stewart RA and Yu W: Identifying tumor suppressors in genetic mosaics: The Drosophila lats gene encodes a putative protein kinase. Development. 121:1053–1063. 1995.PubMed/NCBI

19 

Wang X, Su L and Ou Q: Yes-associated protein promotes tumour development in luminal epithelial derived breast cancer. Eur J Cancer. 48:1227–1234. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Liu Z, Zeng W, Wang S, Zhao X, Guo Y, Yu P, Yin X, Liu C and Huang T: A potential role for the Hippo pathway protein, YAP, in controlling proliferation, cell cycle progression, and autophagy in BCPAP and KI thyroid papillary carcinoma cells. Am J Transl Res. 9:3212–3223. 2017.PubMed/NCBI

21 

Moroishi T, Hansen CG and Guan KL: The emerging roles of YAP and TAZ in cancer. Nat Rev Cancer. 15:73–79. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Mo JS, Park HW and Guan KL: The Hippo signaling pathway in stem cell biology and cancer. EMBO Rep. 15:642–656. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Gao Y, Shi Q, Xu S, Du C, Liang L, Wu K, Wang K, Wang X, Chang LS, He D and Gu P: Curcumin promotes KLF5 proteasome degradation through downregulating YAP/TAZ in bladder cancer cells. Int J Mol Sci. 15:15173–15187. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Oku Y, Nishiya N, Shito T, Yamamoto R, Yamamoto Y, Oyama C and Uehara Y: Small molecules inhibiting the nuclear localization of YAP/TAZ for chemotherapeutics and chemosensitizers against breast cancers. FEBS Open Bio. 5:542–549. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhu J, Zhao L, Luo B and Sheng W: Shikonin regulates invasion and autophagy of cultured colon cancer cells by inhibiting yes‑associated protein. Oncol Lett 18: 6117-6125, 2019.
APA
Zhu, J., Zhao, L., Luo, B., & Sheng, W. (2019). Shikonin regulates invasion and autophagy of cultured colon cancer cells by inhibiting yes‑associated protein. Oncology Letters, 18, 6117-6125. https://doi.org/10.3892/ol.2019.10980
MLA
Zhu, J., Zhao, L., Luo, B., Sheng, W."Shikonin regulates invasion and autophagy of cultured colon cancer cells by inhibiting yes‑associated protein". Oncology Letters 18.6 (2019): 6117-6125.
Chicago
Zhu, J., Zhao, L., Luo, B., Sheng, W."Shikonin regulates invasion and autophagy of cultured colon cancer cells by inhibiting yes‑associated protein". Oncology Letters 18, no. 6 (2019): 6117-6125. https://doi.org/10.3892/ol.2019.10980
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J, Zhao L, Luo B and Sheng W: Shikonin regulates invasion and autophagy of cultured colon cancer cells by inhibiting yes‑associated protein. Oncol Lett 18: 6117-6125, 2019.
APA
Zhu, J., Zhao, L., Luo, B., & Sheng, W. (2019). Shikonin regulates invasion and autophagy of cultured colon cancer cells by inhibiting yes‑associated protein. Oncology Letters, 18, 6117-6125. https://doi.org/10.3892/ol.2019.10980
MLA
Zhu, J., Zhao, L., Luo, B., Sheng, W."Shikonin regulates invasion and autophagy of cultured colon cancer cells by inhibiting yes‑associated protein". Oncology Letters 18.6 (2019): 6117-6125.
Chicago
Zhu, J., Zhao, L., Luo, B., Sheng, W."Shikonin regulates invasion and autophagy of cultured colon cancer cells by inhibiting yes‑associated protein". Oncology Letters 18, no. 6 (2019): 6117-6125. https://doi.org/10.3892/ol.2019.10980
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