KIFC1 promotes the proliferation of hepatocellular carcinoma in vitro and in vivo Retraction in /10.3892/ol.2023.13931

Wang,Xing; Wang,Meng; Li,Xing‑Yue; Li,Jian; Zhao,Dian‑Peng

doi:10.3892/ol.2019.10985

KIFC1 promotes the proliferation of hepatocellular carcinoma in vitro and in vivo

Retraction in: /10.3892/ol.2023.13931

Authors:
- Xing Wang
- Meng Wang
- Xing‑Yue Li
- Jian Li
- Dian‑Peng Zhao
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Affiliations: Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261031, P.R. China
Published online on: October 14, 2019 https://doi.org/10.3892/ol.2019.10985
Pages: 5739-5746
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hepatocellular carcinoma (HCC) is a common type of malignant tumor worldwide with a high mortality rate. In the past 20 years, the morbidity rate of HCC has increased. Progress has been made in the clinical diagnosis and therapy for HCC. However, due to the high heterogeneity and metastasis targeted therapy for HCC exhibits great promise, and novel therapeutic targets for HCC are urgently required. Kinesin family member C1 (KIFC1) is a member of the kinesin superfamily of proteins. Previous studies have indicated a potential association between KIFC1 and cancer progression. However, the potential role of KIFC1 in the development of HCC remains unclear. The present study aimed to explore the function of KIFC1 in HCC. Immunohistochemical (IHC) assays were performed to explore the KIF15 expression levels in 74 samples of HCC and corresponding non‑tumor tissues. The potential association between KIF15 expression levels and clinical features was analyzed, and the effects of KIF15 on cell proliferation of HCC were detected by colony formation and MTT assays. In addition, the proliferation‑related proteins Ki67 and PCNA were detected by western blotting. The possible effects of KIF15 on tumor growth were measured in mice. The results demonstrated that a high expression level of KIFC1 was associated with poor prognosis of HCC. Further results indicated that KIFC1 promoted cell proliferation of HCC in vitro. In addition, knockdown of KIFC1 suppressed tumor formation and growth in mice. Therefore, these results provide a potential therapeutic target for the treatment of HCC.

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1	Dhanasekaran R and Talwalkar JA: Quality of cancer care in patients with cirrhosis and hepatocellular carcinoma. Curr Gastroenterol Rep. 17:342015. View Article : Google Scholar : PubMed/NCBI
2	Lertpipopmetha K and Auewarakul CU: High incidence of hepatitis B infection-associated cirrhosis and hepatocellular carcinoma in the Southeast Asian patients with portal vein thrombosis. BMC Gastroenterol. 11:662011. View Article : Google Scholar : PubMed/NCBI
3	Ziogas IA and Tsoulfas G: Advances and challenges in laparoscopic surgery in the management of hepatocellular carcinoma. World J Gastrointest Surg. 9:233–245. 2017. View Article : Google Scholar : PubMed/NCBI
4	Schietroma I, Scheri GC, Pinacchio C, Statzu M, Petruzziello A and Vullo V: Hepatitis C virus and hepatocellular carcinoma: Pathogenetic mechanisms and impact of direct-acting antivirals. Open Virol J. 12:16–25. 2018. View Article : Google Scholar : PubMed/NCBI
5	Kim D, Li AA, Perumpail BJ, Gadiparthi C, Kim W, Cholankeril G, Glenn JS, Harrison SA, Younossi ZM and Ahmed A: Changing trends in etiology-based and ethnicity-based annual mortality rates of cirrhosis and hepatocellular carcinoma in the United States. Hepatology. 69:1064–1074. 2019. View Article : Google Scholar : PubMed/NCBI
6	Lee HY, Jung JH, Kang YS, Kim YS, Moon HS, Park KO, Lee YS, Kim SM, Seo SW, Lee SW, et al: Clinical significance of transiently elevated serum AFP level in developing hepatocellular carcinoma in HBsAg positive-liver cirrhosis. Korean J Gastroenterol. 43:252–259. 2004.(In Korean). PubMed/NCBI
7	Rasool M, Rashid S, Arooj M, Ansari SA, Khan KM, Malik A, Naseer MI, Zahid S, Manan A, Asif M, et al: New possibilities in hepatocellular carcinoma treatment. Anticancer Res. 34:1563–1571. 2014.PubMed/NCBI
8	Ando E, Tanaka M, Yamashita F, Kuromatsu R, Takada A, Fukumori K, Yano Y, Sumie S, Okuda K, Kumashiro R and Sata M: Diagnostic clues for recurrent hepatocellular carcinoma: Comparison of tumour markers and imaging studies. Eur J Gastroenterol Hepatol. 15:641–648. 2003. View Article : Google Scholar : PubMed/NCBI
9	Aggarwal M, Arain A and Jin Z: Systemic treatment for hepatocellular carcinoma. Chronic Dis Transl Med. 4:148–155. 2018. View Article : Google Scholar : PubMed/NCBI
10	Huang A, Zhao X, Yang XR, Li FQ, Zhou XL, Wu K, Zhang X, Sun QM, Cao Y, Zhu HM, et al: Circumventing intratumoral heterogeneity to identify potential therapeutic targets in hepatocellular carcinoma. J Hepatol. 67:293–301. 2017. View Article : Google Scholar : PubMed/NCBI
11	Ma DD, Pan MY, Hou CC, Tan FQ and Yang WX: KIFC1 and myosin Va: Two motors for acrosomal biogenesis and nuclear shaping during spermiogenesis of Portunus trituberculatus. Cell Tissue Res. 369:625–640. 2017. View Article : Google Scholar : PubMed/NCBI
12	She ZY, Pan MY, Tan FQ and Yang WX: Minus end-directed kinesin-14 KIFC1 regulates the positioning and architecture of the Golgi apparatus. Oncotarget. 8:36469–36483. 2017. View Article : Google Scholar : PubMed/NCBI
13	Kim N and Song K: KIFC1 is essential for bipolar spindle formation and genomic stability in the primary human fibroblast IMR-90 cell. Cell Struct Funct. 38:21–30. 2013. View Article : Google Scholar : PubMed/NCBI
14	Lee SH, Joo K, Jung EJ, Hong H, Seo J and Kim J: Export of membrane proteins from the Golgi complex to the primary cilium requires the kinesin motor, KIFC1. FASEB J. 32:957–968. 2018. View Article : Google Scholar : PubMed/NCBI
15	Yang WX and Sperry AO: C-terminal kinesin motor KIFC1 participates in acrosome biogenesis and vesicle transport. Biol Reprod. 69:1719–1729. 2003. View Article : Google Scholar : PubMed/NCBI
16	Hou CC and Yang WX: Acroframosome-dependent KIFC1 facilitates acrosome formation during spermatogenesis in the caridean shrimp Exopalaemon modestus. PLoS One. 8:e760652013. View Article : Google Scholar : PubMed/NCBI
17	Liu Y, Zhan P, Zhou Z, Xing Z, Zhu S, Ma C, Li Q, Zhu Q, Miao Y, Zhang J, et al: The overexpression of KIFC1 was associated with the proliferation and prognosis of non-small cell lung cancer. J Thorac Dis. 8:2911–2923. 2016. View Article : Google Scholar : PubMed/NCBI
18	Li G, Chong T, Yang J, Li H and Chen H: Kinesin motor protein KIFC1 is a target protein of miR-338-3p and associated with poor prognosis and progression of renal cell carcinoma. Oncol Res. 27:125–137. 2018. View Article : Google Scholar : PubMed/NCBI
19	Li Y, Lu W, Chen D, Boohaker RJ, Zhai L, Padmalayam I, Wennerberg K, Xu B and Zhang W: KIFC1 is a novel potential therapeutic target for breast cancer. Cancer Biol Ther. 16:1316–1322. 2015. View Article : Google Scholar : PubMed/NCBI
20	Sekino Y, Oue N, Shigematsu Y, Ishikawa A, Sakamoto N, Sentani K, Teishima J, Matsubara A and Yasui W: KIFC1 induces resistance to docetaxel and is associated with survival of patients with prostate cancer. Urol Oncol. 35:31.e13–31.e20. 2017. View Article : Google Scholar
21	Xiao YX and Yang WX: KIFC1: A promising chemotherapy target for cancer treatment? Oncotarget. 7:48656–48670. 2016. View Article : Google Scholar : PubMed/NCBI
22	Han J, Wang F, Lan Y, Wang J, Nie C, Liang Y, Song R, Zheng T, Pan S, Pei T, et al: KIFC1 regulated by miR-532-3p promotes epithelial-to-mesenchymal transition and metastasis of hepatocellular carcinoma via gankyrin/AKT signaling. Oncogene. 38:406–420. 2019. View Article : Google Scholar : PubMed/NCBI
23	Seino S, Tsuchiya A, Watanabe Y, Kawata Y, Kojima Y, Ikarashi S, Yanai H, Nakamura K, Kumaki D, Hirano M, et al: Clinical outcome of hepatocellular carcinoma can be predicted by the expression of hepatic progenitor cell markers and serum tumour markers. Oncotarget. 9:21844–21860. 2018. View Article : Google Scholar : PubMed/NCBI
24	Hiraoka A, Michitaka K, Kumada T and Kudo M: ALBI score as a novel tool in staging and treatment planning for hepatocellular carcinoma: Advantage of ALBI grade for universal assessment of hepatic function. Liver Cancer. 6:377–379. 2017. View Article : Google Scholar : PubMed/NCBI
25	DeLeon TT, Ahn DH, Bogenberger JM, Anastasiadis PZ, Arora M, Ramanathan RK, Aqel BA, Vasmatzis G, Truty MJ, Oklu R, et al: Novel targeted therapy strategies for biliary tract cancers and hepatocellular carcinoma. Future Oncol. 14:553–566. 2018. View Article : Google Scholar : PubMed/NCBI
26	Cancer Genome Atlas Research Network. Electronic address: wheeler@bcm.edu, . Cancer Genome Atlas Research Network: Comprehensive and integrative genomic characterization of hepatocellular carcinoma. Cell. 169:1327–1341.e23. 2017. View Article : Google Scholar : PubMed/NCBI
27	Gao HJ, Zhao MC, Zhang YJ, Zhou DS, Xu L, Li GB, Chen MS and Liu J: Monocarboxylate transporter 4 predicts poor prognosis in hepatocellular carcinoma and is associated with cell proliferation and migration. J Cancer Res Clin Oncol. 141:1151–1162. 2015. View Article : Google Scholar : PubMed/NCBI
28	Xiao YX, Shen HQ, She ZY, Sheng L, Chen QQ, Chu YL, Tan FQ and Yang WX: C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma. Oncotarget. 8:61373–61384. 2017. View Article : Google Scholar : PubMed/NCBI
29	Mittal K, Choi DH, Klimov S, Pawar S, Kaur R, Mitra AK, Gupta MV, Sams R, Cantuaria G, Rida PCG and Aneja R: A centrosome clustering protein, KIFC1, predicts aggressive disease course in serous ovarian adenocarcinomas. J Ovarian Res. 9:172016. View Article : Google Scholar : PubMed/NCBI
30	Marsman M, Jordens I, Kuijl C, Janssen L and Neefjes J: Dynein-mediated vesicle transport controls intracellular Salmonella replication. Mol Biol Cell. 15:2954–2964. 2004. View Article : Google Scholar : PubMed/NCBI
31	Huang X, Liu F, Zhu C, Cai J, Wang H, Wang X, He S, Liu C, Yao L, Ding Z, et al: Suppression of KIF3B expression inhibits human hepatocellular carcinoma proliferation. Dig Dis Sci. 59:795–806. 2014. View Article : Google Scholar : PubMed/NCBI
32	Yang T, Zhang XB and Zheng ZM: Suppression of KIF14 expression inhibits hepatocellular carcinoma progression and predicts favorable outcome. Cancer Sci. 104:552–557. 2013. View Article : Google Scholar : PubMed/NCBI
33	Wang J, Ma S, Ma R, Qu X, Liu W, Lv C, Zhao S and Gong Y: KIF2A silencing inhibits the proliferation and migration of breast cancer cells and correlates with unfavorable prognosis in breast cancer. BMC Cancer. 14:4612014. View Article : Google Scholar : PubMed/NCBI