Open Access

Cytokine‑induced killer cells/natural killer cells combined with anti‑GD2 monoclonal antibody increase cell death rate in neuroblastoma SK‑N‑SH cells

  • Authors:
    • Chi Zhang
    • Xilin Xiong
    • Yang Li
    • Ke Huang
    • Ling Liu
    • Xiaomin Peng
    • Wenjun Weng
  • View Affiliations

  • Published online on: October 29, 2019     https://doi.org/10.3892/ol.2019.11020
  • Pages: 6525-6535
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Neuroblastoma (NB) is one of the most common extracranial, solid, pediatric malignancies. Despite improvements in conventional therapies, including surgery, chemotherapy and radiation therapy, the prognosis of stage IV NB remains poor, indicating that novel treatment strategies are required. Immunotherapies, such as anti‑GD2 monoclonal antibodies, used alone or in combination with cytokines, and peripheral blood mononuclear cells or cord blood mononuclear cells (CBMNCs), have been indicated to cause NB cell death and to prolong patient survival in high‑risk NB; however, they remain limited by severe cytotoxicity and side effects. In the present study, it was determined that anti‑GD2 monoclonal antibody alone or CBMNC‑isolated cytokine‑induced killer (CIK)/natural killer (NK) cells alone significantly induced cell death of NB SK‑N‑SH cells, and the combination of anti‑GD2 antibody and CIK/NK cells could significantly increase the cell death rate compared with either treatment alone. In addition, based on a method referred to our previous study, it was identified that a two‑cytokine culture system, using interleukin IL‑2 and IL‑7, effectively stimulated the proliferation of CIK/NK cells. These results serve to suggest a novel treatment strategy for relapsed/refractory NB with high efficiency and few side effects.
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December-2019
Volume 18 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang C, Xiong X, Li Y, Huang K, Liu L, Peng X and Weng W: Cytokine‑induced killer cells/natural killer cells combined with anti‑GD2 monoclonal antibody increase cell death rate in neuroblastoma SK‑N‑SH cells. Oncol Lett 18: 6525-6535, 2019
APA
Zhang, C., Xiong, X., Li, Y., Huang, K., Liu, L., Peng, X., & Weng, W. (2019). Cytokine‑induced killer cells/natural killer cells combined with anti‑GD2 monoclonal antibody increase cell death rate in neuroblastoma SK‑N‑SH cells. Oncology Letters, 18, 6525-6535. https://doi.org/10.3892/ol.2019.11020
MLA
Zhang, C., Xiong, X., Li, Y., Huang, K., Liu, L., Peng, X., Weng, W."Cytokine‑induced killer cells/natural killer cells combined with anti‑GD2 monoclonal antibody increase cell death rate in neuroblastoma SK‑N‑SH cells". Oncology Letters 18.6 (2019): 6525-6535.
Chicago
Zhang, C., Xiong, X., Li, Y., Huang, K., Liu, L., Peng, X., Weng, W."Cytokine‑induced killer cells/natural killer cells combined with anti‑GD2 monoclonal antibody increase cell death rate in neuroblastoma SK‑N‑SH cells". Oncology Letters 18, no. 6 (2019): 6525-6535. https://doi.org/10.3892/ol.2019.11020