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Article

The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol

  • Authors:
    • Chun‑Jung Cho
    • Ching‑Wei Yang
    • Chia‑Lun Wu
    • Jar‑Yi Ho
    • Cheng‑Ping Yu
    • Sheng‑Tang Wu
    • Dah‑Shyong Yu
  • View Affiliations / Copyright

    Affiliations: Graduate Institute of Pathology and Parasitology, National Defense Medical Center, Taipei 114, Taiwan, R.O.C., Department of Urology, Cheng‑Hsin General Hospital, Taipei 112, Taiwan, R.O.C., Division of Urology, Department of Surgery, Tri‑Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan, R.O.C.
  • Pages: 6869-6876
    |
    Published online on: October 30, 2019
       https://doi.org/10.3892/ol.2019.11023
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Abstract

Gemcitabine (GCB), which functions via the inhibition of DNA synthesis, is commonly used in the treatment of bladder cancer; however, its response rate is not satisfactory due to the development of drug resistance. The potential for phytochemicals to reverse drug resistance in bladder cancer tumor cells was evaluated. A human bladder cancer cell line, T24, was cultured, and GCB‑resistant cells (T24‑GCB) were also established. The acquired resistance of T24‑GCB to GCB was measured using an MTT assay. The gene expression of ATP‑binding cassette (ABC) transporter protein family members was analyzed using reverse transcription‑quantitative PCR analysis, and western blotting was performed to verify ABC family protein, cytoplasmic thymidine kinase (TK) and poly (ADP‑ribose) polymerase (PARP) expression on whole cell lysates. Subsequently, resveratrol and curcumin were used to evaluate their modulation potential in decreasing the drug resistance of T24‑GCB cells to GCB using MTT and migration assays. T24‑GCB cells have increased drug resistance ability, with an 18.75‑fold higher ID50 value compared with native T24 cells (105 vs. 5.6 nM). T24‑GCB cells also exhibit increased cross resistance to mitomycin C and paclitaxel. The mRNA expression of ABCC2 in T24‑GCB cells increased compared with that in native T24 cells. Via western blot analysis, it was determined that the expression of ABCC2 protein was also increased in T24‑GCB cells. Conversely, the expression of ABCB1, ABCG2, deoxycytidine kinase (DCK), TK1 and TK2 decreased. Following curcumin and resveratrol treatment alone or combined with GCB, additive cytotoxic enhancement was observed, and the migratory abilities of T24‑GCB cells were significantly decreased. Western blot analysis revealed that ABCC2 protein expression increased, and DCK, TK1 and TK2 expression decreased following co‑treatment of T24‑GCB cells with GCB + curcumin or resveratrol compared with GCB alone. Of note, there was a marked increase in cleaved‑PARP expression in T24‑GCB cells treated with a combination of GCB + curcumin or resveratrol. Both curcumin and resveratrol could reverse the drug resistance of T24‑GCB cells in an additive pattern though PARP enhancement without changes in ABCC2 and DCK, TK1 and TK2 expression.
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1 

Luqmani YA: Mechanisms of drug resistance in cancer chemotherapy. Med Princ Pract. 14 (Suppl 1):S35–S48. 2005. View Article : Google Scholar

2 

Nguyen DP and Thalmann GN: Contemporary update on neoadjuvant therapy for bladder cancer. Nat Rev Urol. 14:348–358. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Chai S, To KK and Lin G: Circumvention of multi-drug resistance of cancer cells by Chinese herbal medicines. Chin Med. 5:262010. View Article : Google Scholar : PubMed/NCBI

4 

Vasiliou V, Vasiliou K and Nebert DW: Human ATP-binding cassette (ABC) transporter family. Hum Genomics. 3:281–290. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Chen Z, Shi T, Zhang L, Zhu P, Deng M, Huang C, Hu T, Jiang L and Li J: Mammalian drug efflux transporters of the ATP binding cassette (ABC) family in multidrug resistance: A review of the past decade. Cancer Lett. 370:153–164. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Fedier A, Dedes KJ, Imesch P, Von Bueren AO and Fink D: The histone deacetylase inhibitors suberoylanilide hydroxamic (vorinostat) and valproic acid induce irreversible and MDR1-independent resistance in human colon cancer cells. Int J Oncol. 31:633–641. 2007.PubMed/NCBI

7 

Collett GP, Robson CN, Mathers JC and Campbell FC: Curcumin modifies Apc(min) apoptosis resistance and inhibits 2-amino 1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) induced tumour formation in Apc(min) mice. Carcinogenesis. 22:821–825. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Chen KG and Sikic BI: Molecular pathways: Regulation and therapeutic implications of multidrug resistance. Clin Cancer Res. 18:1863–1869. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Sapienza C and Issa JP: Diet, nutrition, and cancer epigenetics. Annu Rev Nutr. 36:665–681. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Collins AR, Azqueta A and Langie SA: Effects of micronutrients on DNA repair. Eur J Nutr. 51:261–279. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Royston KJ and Tollefsbol TO: The epigenetic impact of cruciferous vegetables on cancer prevention. Curr Pharmacol Rep. 1:46–51. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Wattanawongdon W, Hahnvajanawong C, Namwat N, Kanchanawat S, Boonmars T, Jearanaikoon P, Leelayuwat C, Techasen A and Seubwai W: Establishment and characterization of gemcitabine-resistant human cholangiocarcinoma cell lines with multidrug resistance and enhanced invasiveness. Int J Oncol. 47:398–410. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Achiwa H, Oguri T, Sato S, Maeda H, Niimi T and Ueda R: Determinants of sensitivity and resistance to gemcitabine: The roles of human equilibrative nucleoside transporter 1 and deoxycytidine kinase in non-small cell lung cancer. Cancer Sci. 95:753–757. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Davidson JD, Ma L, Flagella M, Geeganage S, Gelbert LM and Slapak CA: An increase in the expression of ribonucleotide reductase large subunit 1 is associated with gemcitabine resistance in non-small cell lung cancer cell lines. Cancer Res. 64:3761–3766. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Modok S, Mellor HR and Callaghan R: Modulation of multidrug resistance efflux pump activity to overcome chemoresistance in cancer. Curr Opin Pharmacol. 6:350–354. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Zaman GJ, Flens MJ, van Leusden MR, de Haas M, Mülder HS, Lankelma J, Pinedo HM, Scheper RJ, Baas F, Broxterman HJ, et al: The human multidrug resistance-associated protein MRP is a plasma membrane drug-efflux pump. Proc Natl Acad Sci USA. 91:8822–8826. 1994. View Article : Google Scholar : PubMed/NCBI

18 

Sodani K, Patel A, Kathawala RJ and Chen ZS: Multidrug resistance associated proteins in multidrug resistance. Chin J Cancer. 31:58–72. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Chen ZS and Tiwari AK: Multidrug resistance proteins (MRPs/ABCCs) in cancer chemotherapy and genetic diseases. FEBS J. 278:3226–3245. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Shehzad A, Wahid F and Lee YS: Curcumin in cancer chemoprevention: Molecular targets, pharmacokinetics, bioavailability, and clinical trials. Arch Pharm (Weinheim). 343:489–499. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Lee DS, Lee MK and Kim JH: Curcumin induces cell cycle arrest and apoptosis in human osteosarcoma (HOS) cells. Anticancer Res. 29:5039–5044. 2009.PubMed/NCBI

22 

Guan F, Ding Y, Zhang Y, Zhou Y, Li M and Wang C: Curcumin suppresses proliferation and migration of MDA-MB-231 breast cancer cells through autophagy-dependent Akt degradation. PLoS One. 11:e01465532016. View Article : Google Scholar : PubMed/NCBI

23 

Limtrakul P, Anuchapreeda S and Buddhasukh D: Modulation of human multidrug-resistance MDR-1 gene by natural curcuminoids. BMC Cancer. 4:132004. View Article : Google Scholar : PubMed/NCBI

24 

Tang XQ, Bi H, Feng JQ and Cao JG: Effect of curcumin on multidrug resistance in resistant human gastric carcinoma cell line SGC7901/VCR. Acta Pharmacol Sin. 26:1009–1016. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Patel BB and Majumdar AP: Synergistic role of curcumin with current therapeutics in colorectal cancer: Minireview. Nutr Cancer. 61:842–846. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Mukhtar E, Adhami VM, Khan N and Mukhtar H: Apoptosis and autophagy induction as mechanism of cancer prevention by naturally occurring dietary agents. Curr Drug Targets. 13:1831–1841. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Varoni EM, Lo Faro AF, Sharifi-Rad J and Iriti M: Anticancer molecular mechanisms of resveratrol. Front Nutr. 3:82016. View Article : Google Scholar : PubMed/NCBI

28 

Yousef M, Vlachogiannis IA and Tsiani E: Effects of resveratrol against lung cancer: In vitro and in vivo studies. Nutrients. 9:E12312017. View Article : Google Scholar : PubMed/NCBI

29 

Lin HY, Shih A, Davis FB, Tang HY, Martino LJ, Bennett JA and Davis PJ: Resveratrol induced serine phosphorylation of p53 causes apoptosis in a mutant p53 prostate cancer cell line. J Urol. 168:748–755. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Holian O, Wahid S, Atten MJ and Attar BM: Inhibition of gastric cancer cell proliferation by resveratrol: Role of nitric oxide. Am J Physiol Gastrointest Liver Physiol. 282:G809–G816. 2002. View Article : Google Scholar : PubMed/NCBI

31 

Manna SK, Mukhopadhyay A and Aggarwal BB: Resveratrol suppresses TNF-induced activation of nuclear transcription factors NF-kappa B, activator protein-1, and apoptosis: Potential role of reactive oxygen intermediates and lipid peroxidation. J Immunol. 164:6509–6519. 2000. View Article : Google Scholar : PubMed/NCBI

32 

Wang Y, Romigh T, He X, Orloff MS, Silverman RH, Heston WD and Eng C: Resveratrol regulates the PTEN/AKT pathway through androgen receptor-dependent and -independent mechanisms in prostate cancer cell lines. Hum Mol Genet. 19:4319–4329. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cho CJ, Yang CW, Wu CL, Ho JY, Yu CP, Wu ST and Yu DS: The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol. Oncol Lett 18: 6869-6876, 2019.
APA
Cho, C., Yang, C., Wu, C., Ho, J., Yu, C., Wu, S., & Yu, D. (2019). The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol. Oncology Letters, 18, 6869-6876. https://doi.org/10.3892/ol.2019.11023
MLA
Cho, C., Yang, C., Wu, C., Ho, J., Yu, C., Wu, S., Yu, D."The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol". Oncology Letters 18.6 (2019): 6869-6876.
Chicago
Cho, C., Yang, C., Wu, C., Ho, J., Yu, C., Wu, S., Yu, D."The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol". Oncology Letters 18, no. 6 (2019): 6869-6876. https://doi.org/10.3892/ol.2019.11023
Copy and paste a formatted citation
x
Spandidos Publications style
Cho CJ, Yang CW, Wu CL, Ho JY, Yu CP, Wu ST and Yu DS: The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol. Oncol Lett 18: 6869-6876, 2019.
APA
Cho, C., Yang, C., Wu, C., Ho, J., Yu, C., Wu, S., & Yu, D. (2019). The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol. Oncology Letters, 18, 6869-6876. https://doi.org/10.3892/ol.2019.11023
MLA
Cho, C., Yang, C., Wu, C., Ho, J., Yu, C., Wu, S., Yu, D."The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol". Oncology Letters 18.6 (2019): 6869-6876.
Chicago
Cho, C., Yang, C., Wu, C., Ho, J., Yu, C., Wu, S., Yu, D."The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol". Oncology Letters 18, no. 6 (2019): 6869-6876. https://doi.org/10.3892/ol.2019.11023
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