Open Access

Differentiation and roles of bone marrow‑derived cells on the tumor microenvironment of oral squamous cell carcinoma

  • Authors:
    • Chang Anqi
    • Kiyofumi Takabatake
    • Hotaka Kawai
    • May Wathone Oo
    • Saori Yoshida
    • Masae Fujii
    • Haruka Omori
    • Shintaro Sukegawa
    • Keisuke Nakano
    • Hidetsugu Tsujigiwa
    • Zheng Jinhua
    • Hitoshi Nagatsuka
  • View Affiliations

  • Published online on: November 4, 2019     https://doi.org/10.3892/ol.2019.11045
  • Pages: 6628-6638
  • Copyright: © Anqi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The stroma affects the properties and dynamics of the tumor. Previous studies have demonstrated that bone marrow‑derived cells (BMDCs) possess the capability of differentiating into stromal cells. However, the characteristics and roles of BMDCs in oral squamous cell carcinoma remain unclear. The current study therefore investigated their locations and features by tracing green fluorescent protein (GFP)‑labeled BMDCs in a transplantation mouse model. After irradiation, BALB‑c nu‑nu mice were injected with bone marrow cells from C57BL/6‑BALB‑C‑nu/nu‑GFP transgenic mice. These recipient mice were then injected subcutaneously in the head with human squamous cell carcinoma‑2 cells. Immunohistochemistry for GFP, Vimentin, CD11b, CD31 and α‑smooth muscle actin (SMA), and double‑fluorescent immunohistochemistry for GFP‑Vimentin, GFP‑CD11b, GFP‑CD31 and GFP‑α‑SMA was subsequently performed. Many round‑shaped GFP‑positive cells were observed in the cancer stroma, which indicated that BMDCs served a predominant role in tumorigenesis. Vimentin(+) GFP(+) cells may also be a member of the cancer‑associated stroma, originating from bone marrow. Round or spindle‑shaped CD11b(+) GFP(+) cells identified in the present study may be macrophages derived from bone marrow. CD31(+)GFP(+) cells exhibited a high tendency towards bone marrow‑derived angioblasts. The results also indicated that spindle‑shaped α‑SMA(+) GFP(+) cells were not likely to represent bone marrow‑derived cancer‑associated fibroblasts. BMDCs gathering within the tumor microenvironment exhibited multilineage potency and participated in several important processes, such as tumorigenesis, tumor invasion and angiogenesis.
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December-2019
Volume 18 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Anqi C, Takabatake K, Kawai H, Oo MW, Yoshida S, Fujii M, Omori H, Sukegawa S, Nakano K, Tsujigiwa H, Tsujigiwa H, et al: Differentiation and roles of bone marrow‑derived cells on the tumor microenvironment of oral squamous cell carcinoma. Oncol Lett 18: 6628-6638, 2019
APA
Anqi, C., Takabatake, K., Kawai, H., Oo, M.W., Yoshida, S., Fujii, M. ... Nagatsuka, H. (2019). Differentiation and roles of bone marrow‑derived cells on the tumor microenvironment of oral squamous cell carcinoma. Oncology Letters, 18, 6628-6638. https://doi.org/10.3892/ol.2019.11045
MLA
Anqi, C., Takabatake, K., Kawai, H., Oo, M. W., Yoshida, S., Fujii, M., Omori, H., Sukegawa, S., Nakano, K., Tsujigiwa, H., Jinhua, Z., Nagatsuka, H."Differentiation and roles of bone marrow‑derived cells on the tumor microenvironment of oral squamous cell carcinoma". Oncology Letters 18.6 (2019): 6628-6638.
Chicago
Anqi, C., Takabatake, K., Kawai, H., Oo, M. W., Yoshida, S., Fujii, M., Omori, H., Sukegawa, S., Nakano, K., Tsujigiwa, H., Jinhua, Z., Nagatsuka, H."Differentiation and roles of bone marrow‑derived cells on the tumor microenvironment of oral squamous cell carcinoma". Oncology Letters 18, no. 6 (2019): 6628-6638. https://doi.org/10.3892/ol.2019.11045